Alexa Fluor® 488 anti-NFATc1 | BioLegend 649603 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Alexa Fluor® 488 anti-NFATc1

catalog :

649603

quantity :

25 μg

price :

147 USD

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

7A6

reactivity :

human, mouse, rat

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; fig 4c	Leclerc M, Voilin E, Gros G, Corgnac S, de Montpreville V, Validire P, et al. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1. Nat Commun. 2019;10:3345 pubmed publisher
	Trefzer A, Kadam P, Wang S, Pennavaria S, Lober B, Akçabozan B, et al. Dynamic adoption of anergy by antigen-exhausted CD4+ T cells. Cell Rep. 2021;34:108748 pubmed publisher
	Morelli A, Sumpter T, Rojas Canales D, Bandyopadhyay M, Chen Z, Tkacheva O, et al. Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells. Cell Rep. 2020;30:3448-3465.e8 pubmed publisher
	Kannan S, Aitken M, Herbrich S, Golfman L, Hall M, Mak D, et al. Anti-Leukemia Effects of Notch-Mediated Inhibition of Oncogenic PLK1 in B-Cell Acute Lymphoblastic Leukemia. Mol Cancer Ther. 2019;: pubmed publisher
	Nonomura K, Lukacs V, Sweet D, Goddard L, Kanie A, Whitwam T, et al. Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation. Proc Natl Acad Sci U S A. 2018;115:12817-12822 pubmed publisher
	Cuadrado E, van den Biggelaar M, de Kivit S, Chen Y, Slot M, Doubal I, et al. Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity. Immunity. 2018;48:1046-1059.e6 pubmed publisher
	Guichard V, Bonilla N, Durand A, Audemard Verger A, Guilbert T, Martin B, et al. Calcium-mediated shaping of naive CD4 T-cell phenotype and function. elife. 2017;6: pubmed publisher
	Hoffmann U, Neudörfl C, Daemen K, Keil J, Stevanovic Meyer M, Lehner F, et al. NK Cells of Kidney Transplant Recipients Display an Activated Phenotype that Is Influenced by Immunosuppression and Pathological Staging. PLoS ONE. 2015;10:e0132484 pubmed publisher
	Fan W, Liang D, Tang Y, Qu B, Cui H, Luo X, et al. Identification of microRNA-31 as a novel regulator contributing to impaired interleukin-2 production in T cells from patients with systemic lupus erythematosus. Arthritis Rheum. 2012;64:3715-25 pubmed publisher

product information

Antigen :

NFATc1

Apps. Abbrev. :

ICFC

Cat # :

649603

Clone :

7A6

Item :

Alexa Fluor® 488 anti-NFATc1

Isotype :

Mouse IgG1, κ

Other Names :

Nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, NFAT2, NFATc

Size :

25 μg

Price (USD) :

147 USD

Reactivity :

Human, Mouse, Rat

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

A488

Immunogen :

Recombinant protein of human NFATc1 amino acids 197-304.

Application Notes :

7A6 antibody detects endogenous human NFATC1 in Western blot with an approximate molecular weight of 100 - 120 kD (different isoforms) (Cat. No. 649602). The optimal concentration should be determined by titration for each individual assay of interest.; Clone 7A6 Intracellular Staining Procedure: ;Prepare the fix/perm solution, consisting of 1% paraformaldehyde in 70% ethanol. Chill for at least one hour at -20°C.;Suspend cells in cell staining buffer and perform surface stain if necessary.;Centrifuge for 5 minutes at 1500 RPM and discard supernatant.;Resuspend cells in 500 µL of fix/perm solution for up to 2 x 10^6 cells and gently vortex.;Incubate cells on ice for 30-60 minutes in the dark.;Add 2 mL PBS and centrifuge cells for 1500 RPM for 5 minutes.;Wash cells 1X with 2 mL cell staining buffer, centrifuge at 1500 RPM for 5 minutes, discard supernatant.;Adjust cell concentration to about 1 x 10^7 cells/mL, add 100 µL (about 1 x 10^6 cells) to a FACS tube that already contains the staining antibody and mix gently.;Incubate 15 minutes at room temperature.;Wash 2X with cell staining buffer as in step 6.;Resuspend cells in cell staining buffer and analyze.;

more info or order :

BioLegend product webpage