Purified anti-NFATc1 | BioLegend 649601 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-NFATc1

catalog :

649601

quantity :

25 μg

price :

124 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7A6

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, chromatin immunoprecipitation

more info or order :

BioLegend product webpage

citations: 4

Reference
Brummer G, Fang W, Smart C, Zinda B, Alissa N, Berkland C, et al. CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments. Oncogene. 2020;39:2275-2289 pubmed publisher
Li X, Islam S, Xiong M, Nsumu N, Lee M, Zhang L, et al. Epigenetic regulation of NfatC1 transcription and osteoclastogenesis by nicotinamide phosphoribosyl transferase in the pathogenesis of arthritis. Cell Death Discov. 2019;5:62 pubmed publisher
Murata K, Fang C, Terao C, Giannopoulou E, Lee Y, Lee M, et al. Hypoxia-Sensitive COMMD1 Integrates Signaling and Cellular Metabolism in Human Macrophages and Suppresses Osteoclastogenesis. Immunity. 2017;47:66-79.e5 pubmed publisher
Fan W, Liang D, Tang Y, Qu B, Cui H, Luo X, et al. Identification of microRNA-31 as a novel regulator contributing to impaired interleukin-2 production in T cells from patients with systemic lupus erythematosus. Arthritis Rheum. 2012;64:3715-25 pubmed publisher

product information

Antigen :

NFATc1

Apps. Abbrev. :

WB, FC, ICC, ChIP

Cat # :

649601

Clone :

7A6

Item :

Purified anti-NFATc1

Isotype :

Mouse IgG1, κ

Other Names :

Nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, NFAT2, NFATc

Size :

25 μg

Price (USD) :

124 USD

Reactivity :

Human, Mouse, Rat

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PURE

Immunogen :

Recombinant protein of human NFATc1 amino acids 197-304.

Application Notes :

7A6 antibody detects endogenous human NFATC1 in Western blot. There are 10 isoforms with a predicted MW of 39kD, 74kD, 76kD, 77kD (3), 88kD (2), 100kD, 101kD. With this antibody, observed MW bands range from 70 - 120 kD. The optimal concentration should be determined by titration for each individual assay of interest.Clone 7A6 Intracellular Staining Procedure: ;Prepare the fix/perm solution, consisting of 1% paraformaldehyde in 70% ethanol. Chill for at least one hour at -20°C.;Suspend cells in cell staining buffer and perform surface stain if necessary.;Centrifuge for 5 minutes at 1500 RPM and discard supernatant.;Resuspend cells in 500 µL of fix/perm solution for up to 2 x 10^6 cells and gently vortex.;Incubate cells on ice for 30-60 minutes in the dark.;Add 2 mL PBS and centrifuge cells for 1500 RPM for 5 minutes.;Wash cells 1X with 2 mL cell staining buffer, centrifuge at 1500 RPM for 5 minutes, discard supernatant.;Adjust cell concentration to about 1 x 10^7 cells/mL, add 100 µL (about 1 x 10^6 cells) to a FACS tube that already contains the staining antibody and mix gently.;Incubate 15 minutes at room temperature.;Wash 2X with cell staining buffer as in step 6.;Resuspend cells in cell staining buffer and analyze.;

more info or order :

BioLegend product webpage