product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Direct-Blot™ HRP anti-Ubiquitin
catalog :
646303
quantity :
100 μL
price :
340 USD
clonality :
monoclonal
host :
mouse
conjugate :
HRP
clone name :
P4D1
reactivity :
human, brewer's yeast
application :
western blot
more info or order :
citations: 5
Published Application/Species/Sample/DilutionReference
  • western blot; brewer's yeast; loading ...; fig 7b
Defenouillère Q, Namane A, Mouaikel J, Jacquier A, Fromont Racine M. The ribosome-bound quality control complex remains associated to aberrant peptides during their proteasomal targeting and interacts with Tom1 to limit protein aggregation. Mol Biol Cell. 2017;28:1165-1176 pubmed publisher
Nguyen T, Bergmann U, Kietzmann T, Mennerich D. Protein kinase B/AKT phosphorylates hypoxia-inducible factor-3α1 in response to insulin, promoting cell growth and migration. Front Cell Dev Biol. 2023;11:1250000 pubmed publisher
VanDyke D, Xu L, Sargunas P, Gilbreth R, Baca M, Gao C, et al. Redirecting the specificity of tripartite motif containing-21 scaffolds using a novel discovery and design approach. J Biol Chem. 2023;299:105381 pubmed publisher
Goodwin M, Lee E, Lakshmanan U, Shipp S, Froessl L, Barzaghi F, et al. CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cells. Sci Adv. 2020;6:eaaz0571 pubmed publisher
Magnaudeix A, Wilson C, Page G, Bauvy C, Codogno P, Leveque P, et al. PP2A blockade inhibits autophagy and causes intraneuronal accumulation of ubiquitinated proteins. Neurobiol Aging. 2013;34:770-90 pubmed publisher
product information
Antigen :
Ubiquitin
Apps. Abbrev. :
WB
Cat # :
646303
Clone :
P4D1
Item :
Direct-Blot™ HRP anti-Ubiquitin
Isotype :
Mouse IgG1, κ
Size :
100 μL
Price (USD) :
340 USD
Reactivity :
All Species
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
DB_HRP
Immunogen :
Denatured glutaraldehyde cross-linked ubiquitin IgG complex
Application Notes :
This antibody recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins. The ubiquitin protein is extremley well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and ubiquitin-substrate conjugates by immunoblotting. Because the antibody recognizes a denatured epitope, it is known to not work in immunoprecipitation or immunostaining applications.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA