This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Direct-Blot™ HRP anti-T-bet
catalog :
644831
quantity :
100 μL
price :
340 USD
clonality :
monoclonal
host :
mouse
conjugate :
HRP
clone name :
4B10
reactivity :
human, mouse
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s2b
Shi Z, Takeuchi T, Nakanishi Y, Kato T, Beck K, Nagata R, et al. A Japanese Herbal Formula, Daikenchuto, Alleviates Experimental Colitis by Reshaping Microbial Profiles and Enhancing Group 3 Innate Lymphoid Cells. Front Immunol. 2022;13:903459 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7b, s1a
Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Kumar A, Chamoto K, Chowdhury P, Honjo T. Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig e8b
Alspach E, Lussier D, Miceli A, Kizhvatov I, DuPage M, Luoma A, et al. MHC-II neoantigens shape tumour immunity and response to immunotherapy. Nature. 2019;574:696-701 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 4c, 4e, s6c
Ansaldo E, Slayden L, Ching K, Koch M, Wolf N, Plichta D, et al. Akkermansia muciniphila induces intestinal adaptive immune responses during homeostasis. Science. 2019;364:1179-1184 pubmed publisher
  • flow cytometry; mouse; loading ...
Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Kotov J, Kotov D, Linehan J, Bardwell V, Gearhart M, Jenkins M. BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors. J Exp Med. 2019;216:1450-1464 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Alam M, Yang D, Trivett A, Meyer T, Oppenheim J. HMGN1 and R848 Synergistically Activate Dendritic Cells Using Multiple Signaling Pathways. Front Immunol. 2018;9:2982 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3d, 3f
Sato Y, Bolzenius J, Eteleeb A, Su X, Maher C, Sehn J, et al. CD4+ T cells induce rejection of urothelial tumors after immune checkpoint blockade. JCI Insight. 2018;3: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Singh M, Ni M, Sullivan J, Hamerman J, Campbell D. B cell adaptor for PI3-kinase (BCAP) modulates CD8+ effector and memory T cell differentiation. J Exp Med. 2018;215:2429-2443 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Cummings M, Arumanayagam A, Zhao P, Kannanganat S, Stuve O, Karandikar N, et al. Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis. PLoS ONE. 2018;13:e0200752 pubmed publisher
  • flow cytometry; human; loading ...; fig 3d
Hartana C, Ahlén Bergman E, Broome A, Berglund S, Johansson M, Alamdari F, et al. Tissue-resident memory T cells are epigenetically cytotoxic with signs of exhaustion in human urinary bladder cancer. Clin Exp Immunol. 2018;194:39-53 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Arnold I, Artola Borán M, Tallón de Lara P, Kyburz A, Taube C, OTTEMANN K, et al. Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation. J Exp Med. 2018;215:2055-2072 pubmed publisher
  • flow cytometry; human; loading ...; fig 6l
Jung I, Kim Y, Yu H, Lee M, Kim S, Lee J. CRISPR/Cas9-Mediated Knockout of DGK Improves Antitumor Activities of Human T Cells. Cancer Res. 2018;78:4692-4703 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Tinoco R, Carrette F, Henriquez M, Fujita Y, Bradley L. Fucosyltransferase Induction during Influenza Virus Infection Is Required for the Generation of Functional Memory CD4+ T Cells. J Immunol. 2018;200:2690-2702 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6a
Kotov D, Kotov J, Goldberg M, Jenkins M. Many Th Cell Subsets Have Fas Ligand-Dependent Cytotoxic Potential. J Immunol. 2018;200:2004-2012 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Burrack A, Malhotra D, Dileepan T, Osum K, Swanson L, Fife B, et al. Cutting Edge: Allograft Rejection Is Associated with Weak T Cell Responses to Many Different Graft Leukocyte-Derived Peptides. J Immunol. 2018;200:477-482 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5b
Maseda D, Johnson E, Nyhoff L, Baron B, Kojima F, Wilhelm A, et al. mPGES1-Dependent Prostaglandin E2 (PGE2) Controls Antigen-Specific Th17 and Th1 Responses by Regulating T Autocrine and Paracrine PGE2 Production. J Immunol. 2018;200:725-736 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig s5f
Hasan Z, Koizumi S, Sasaki D, Yamada H, Arakaki N, Fujihara Y, et al. JunB is essential for IL-23-dependent pathogenicity of Th17 cells. Nat Commun. 2017;8:15628 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Lino C, Barros Martins J, Oberdörfer L, Walzer T, Prinz I. Eomes expression reports the progressive differentiation of IFN-?-producing Th1-like ?? T cells. Eur J Immunol. 2017;47:970-981 pubmed publisher
  • flow cytometry; human; loading ...; fig s2a
Martin Gayo E, Cronin J, Hickman T, Ouyang Z, Lindqvist M, Kolb K, et al. Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth. JCI Insight. 2017;2:e89574 pubmed publisher
  • flow cytometry; human; loading ...
Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4E
Troegeler A, Mercier I, Cougoule C, Pietretti D, Colom A, Duval C, et al. C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells. Proc Natl Acad Sci U S A. 2017;114:E540-E549 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d
Beyaz S, Kim J, Pinello L, Xifaras M, Hu Y, Huang J, et al. The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells. Nat Immunol. 2017;18:184-195 pubmed publisher
  • flow cytometry; human; loading ...; fig s7
Peters C, Häsler R, Wesch D, Kabelitz D. Human Vδ2 T cells are a major source of interleukin-9. Proc Natl Acad Sci U S A. 2016;113:12520-12525 pubmed
  • flow cytometry; human; loading ...; fig 5a
Demers K, Makedonas G, Buggert M, Eller M, Ratcliffe S, Goonetilleke N, et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog. 2016;12:e1005805 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
  • flow cytometry; human; fig 2
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Ngiow S, Young A, Jacquelot N, Yamazaki T, Enot D, Zitvogel L, et al. A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1. Cancer Res. 2015;75:3800-11 pubmed publisher
  • flow cytometry; mouse; fig 3d
Moguche A, Shafiani S, Clemons C, Larson R, Dinh C, Higdon L, et al. ICOS and Bcl6-dependent pathways maintain a CD4 T cell population with memory-like properties during tuberculosis. J Exp Med. 2015;212:715-28 pubmed publisher
  • flow cytometry; human; fig 5
Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, et al. Effector Vγ9Vδ2 T cells dominate the human fetal γδ T-cell repertoire. Proc Natl Acad Sci U S A. 2015;112:E556-65 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1j
Xia S, Wei J, Wang J, Sun H, Zheng W, Li Y, et al. A requirement of dendritic cell-derived interleukin-27 for the tumor infiltration of regulatory T cells. J Leukoc Biol. 2014;95:733-742 pubmed
Hegde S, Krisnawan V, Herzog B, Zuo C, Breden M, Knolhoff B, et al. Dendritic Cell Paucity Leads to Dysfunctional Immune Surveillance in Pancreatic Cancer. Cancer Cell. 2020;37:289-307.e9 pubmed publisher
Hsieh C, Chen C, Tsai C, Huang W, Tseng P, Lin Y, et al. Inhibiting glycogen synthase kinase-3 decreases 12-O-tetradecanoylphorbol-13-acetate-induced interferon-?-mediated skin inflammation. J Pharmacol Exp Ther. 2012;343:125-33 pubmed publisher
product information
Antigen :
T-bet
Apps. Abbrev. :
WB
Cat # :
644831
Clone :
4B10
Item :
Direct-Blot™ HRP anti-T-bet
Isotype :
Mouse IgG1, κ
Other Names :
T-box expressed in T cells, T box 21, TBLYM
Size :
100 μL
Price (USD) :
340 USD
Reactivity :
Human, Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
DB_HRP
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA