product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-Alix
catalog :
634502
quantity :
100 μg
price :
226 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3A9
reactivity :
human, mouse
application :
western blot, immunocytochemistry, immunoprecipitation, western blot knockout validation
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; 1:1000; loading ...; fig 6a
Claude Taupin A, Jia J, Bhujabal Z, Garfa Traoré M, Kumar S, da Silva G, et al. ATG9A protects the plasma membrane from programmed and incidental permeabilization. Nat Cell Biol. 2021;23:846-858 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1b
Skowyra M, Schlesinger P, Naismith T, Hanson P. Triggered recruitment of ESCRT machinery promotes endolysosomal repair. Science. 2018;360: pubmed publisher
Inouye K, Prentice K, Lee A, Wang Z, Dominguez Gonzalez C, Chen M, et al. Endothelial-derived FABP4 constitutes the majority of basal circulating hormone and regulates lipolysis-driven insulin secretion. JCI Insight. 2023;8: pubmed publisher
Stempels F, Jiang M, Warner H, Moser M, Janssens M, Maassen S, et al. Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters. J Cell Biol. 2023;222: pubmed publisher
Jia X, Knyazeva A, Zhang Y, Castro Gonzalez S, Nakamura S, Carlson L, et al. V. cholerae MakA is a cholesterol-binding pore-forming toxin that induces non-canonical autophagy. J Cell Biol. 2022;221: pubmed publisher
Andr xe9 Gr xe9 goire G, Maghe C, Douanne T, Rosi x144 ska S, Spinelli F, Thys A, et al. Inhibition of the pseudokinase MLKL alters extracellular vesicle release and reduces tumor growth in glioblastoma. iScience. 2022;25:105118 pubmed publisher
Tan J, Finkel T. A phosphoinositide signalling pathway mediates rapid lysosomal repair. Nature. 2022;609:815-821 pubmed publisher
Dong H, Zhao B, Chen J, Liu Z, Li X, Li L, et al. Mitochondrial calcium uniporter promotes phagocytosis-dependent activation of the NLRP3 inflammasome. Proc Natl Acad Sci U S A. 2022;119:e2123247119 pubmed publisher
Yim W, Yamamoto H, Mizushima N. Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair. FEBS Lett. 2022;596:991-1003 pubmed publisher
Millarte V, Schlienger S, Kälin S, Spiess M. Rabaptin5 targets autophagy to damaged endosomes and Salmonella vacuoles via FIP200 and ATG16L1. EMBO Rep. 2022;23:e53429 pubmed publisher
Burbidge K, Rademacher D, Mattick J, Zack S, Grillini A, Bousset L, et al. LGALS3 (galectin 3) mediates an unconventional secretion of SNCA/α-synuclein in response to lysosomal membrane damage by the autophagic-lysosomal pathway in human midbrain dopamine neurons. Autophagy. 2022;18:1020-1048 pubmed publisher
Chand S, Gowen A, Savine M, Moore D, Clark A, Huynh W, et al. A comprehensive study to delineate the role of an extracellular vesicle-associated microRNA-29a in chronic methamphetamine use disorder. J Extracell Vesicles. 2021;10:e12177 pubmed publisher
Gulluni F, Prever L, Li H, Krafčíková P, Corrado I, Lo W, et al. PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation. Science. 2021;374:eabk0410 pubmed publisher
Valkov N, Das A, Tucker N, Li G, Salvador A, Chaffin M, et al. SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart. Life Sci Alliance. 2021;4: pubmed publisher
Baba T, Yoshida T, Tanabe Y, Nishimura T, Morishita S, Gotoh N, et al. Cytoplasmic DNA accumulation preferentially triggers cell death of myeloid leukemia cells by interacting with intracellular DNA sensing pathway. Cell Death Dis. 2021;12:322 pubmed publisher
Hiraga C, Yamamoto S, Hashimoto S, Kasahara M, Minamisawa T, Matsumura S, et al. Pentapartite fractionation of particles in oral fluids by differential centrifugation. Sci Rep. 2021;11:3326 pubmed publisher
Gorji Bahri G, Moradtabrizi N, Vakhshiteh F, Hashemi A. Validation of common reference genes stability in exosomal mRNA-isolated from liver and breast cancer cell lines. Cell Biol Int. 2021;45:1098-1110 pubmed publisher
Davis O, Shin H, Lim C, Wu E, Kukurugya M, Maher C, et al. NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C. Dev Cell. 2021;56:260-276.e7 pubmed publisher
Gorji Bahri G, Moghimi H, Hashemi A. RAB5A is associated with genes involved in exosome secretion: Integration of bioinformatics analysis and experimental validation. J Cell Biochem. 2021;122:425-441 pubmed publisher
Gunasekaran M, Bansal S, Ravichandran R, Sharma M, Perincheri S, Rodriguez F, et al. Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection. J Heart Lung Transplant. 2020;39:379-388 pubmed publisher
Suthar J, Parsons E, Hoogenboom B, Williams G, Guldin S. Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring. Anal Chem. 2020;92:4082-4093 pubmed publisher
Jia J, Bissa B, Brecht L, Allers L, Choi S, Gu Y, et al. AMPK, a Regulator of Metabolism and Autophagy, Is Activated by Lysosomal Damage via a Novel Galectin-Directed Ubiquitin Signal Transduction System. Mol Cell. 2020;77:951-969.e9 pubmed publisher
Jia J, Claude Taupin A, Gu Y, Choi S, Peters R, Bissa B, et al. Galectin-3 Coordinates a Cellular System for Lysosomal Repair and Removal. Dev Cell. 2019;: pubmed publisher
Grandclaudon M, Perrot Dockès M, Trichot C, Karpf L, Abouzid O, Chauvin C, et al. A Quantitative Multivariate Model of Human Dendritic Cell-T Helper Cell Communication. Cell. 2019;179:432-447.e21 pubmed publisher
Hüttenhain R, Xu J, Burton L, Gordon D, Hultquist J, Johnson J, et al. ARIH2 Is a Vif-Dependent Regulator of CUL5-Mediated APOBEC3G Degradation in HIV Infection. Cell Host Microbe. 2019;26:86-99.e7 pubmed publisher
Moore D, Meays B, Madduri L, Shahjin F, Chand S, Niu M, et al. Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder. Stem Cells Int. 2019;2019:8710180 pubmed publisher
Ko J, Hemphill M, Yang Z, Sewell E, Na Y, Sandsmark D, et al. Diagnosis of traumatic brain injury using miRNA signatures in nanomagnetically isolated brain-derived extracellular vesicles. Lab Chip. 2018;18:3617-3630 pubmed publisher
Schiller L, Lemus Diaz N, Rinaldi Ferreira R, Böker K, Gruber J. Enhanced Production of Exosome-Associated AAV by Overexpression of the Tetraspanin CD9. Mol Ther Methods Clin Dev. 2018;9:278-287 pubmed publisher
Gärtner K, Battke C, Dünzkofer J, Hüls C, von Neubeck B, Kellner M, et al. Tumor-derived extracellular vesicles activate primary monocytes. Cancer Med. 2018;7:2013-2020 pubmed publisher
Gunasekaran M, Sharma M, Hachem R, Bremner R, Smith M, Mohanakumar T. Circulating Exosomes with Distinct Properties during Chronic Lung Allograft Rejection. J Immunol. 2018;200:2535-2541 pubmed publisher
Yoon S, Kovalenko A, Bogdanov K, Wallach D. MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation. Immunity. 2017;47:51-65.e7 pubmed publisher
Bæk R, Søndergaard E, Varming K, Jørgensen M. The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray. J Immunol Methods. 2016;438:11-20 pubmed publisher
Ertunc M, Sikkeland J, Fenaroli F, Griffiths G, Daniels M, Cao H, et al. Secretion of fatty acid binding protein aP2 from adipocytes through a nonclassical pathway in response to adipocyte lipase activity. J Lipid Res. 2015;56:423-34 pubmed publisher
Morel L, Regan M, Higashimori H, Ng S, Esau C, Vidensky S, et al. Neuronal exosomal miRNA-dependent translational regulation of astroglial glutamate transporter GLT1. J Biol Chem. 2013;288:7105-16 pubmed publisher
Yang C, Kim S, Bianco N, Robbins P. Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model. PLoS ONE. 2011;6:e22517 pubmed publisher
product information
Antigen :
Alix
Apps. Abbrev. :
WB, ICC, IP
Cat # :
634502
Clone :
3A9
Item :
Purified anti-Alix
Isotype :
Mouse IgG1, κ
Other Names :
PDCD6IP, PDCD6 interacting protein, AIP1, Hp95
Size :
100 μg
Price (USD) :
226 USD
Reactivity :
Human, Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Recombinant full-length human Alix protein
Application Notes :
Clone 3A9 has been shown to be useful for Western blotting^1, immunoprecipitation, and immunofluorescence^2 staining of the human and mouse Alix protein.Clone 3A9 shows strong specificity for mouse Alix.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA