product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-H2A.X Phospho (Ser139)
catalog :
613405
quantity :
25 tests
price :
188 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF488
antigen modification :
phosphorylated
clone name :
2F3
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...
Warnes G. Flow cytometric detection of hyper-polarized mitochondria in regulated and accidental cell death processes. Apoptosis. 2020;25:548-557 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 4b
Okawa A, Morioka T, Imaoka T, Kakinuma S, Matsumoto Y. Differential expression of DNA-dependent protein kinase catalytic subunit in the brain of neonatal mice and young adult mice. Proc Jpn Acad Ser B Phys Biol Sci. 2020;96:171-179 pubmed publisher
Varun K, Zoltan K, Alba S, Manuel B, Elisabeth K, Dimitrios T, et al. Elevated markers of DNA damage and senescence are associated with the progression of albuminuria and restrictive lung disease in patients with type 2 diabetes. EBioMedicine. 2023;90:104516 pubmed publisher
Zhang Z, Samsa W, De Y, Zhang F, Reizes O, Almasan A, et al. HDGFRP3 interaction with 53BP1 promotes DNA double-strand break repair. Nucleic Acids Res. 2023;51:2238-2256 pubmed publisher
Pelicci S, Furia L, Pelicci P, Faretta M. Correlative Multi-Modal Microscopy: A Novel Pipeline for Optimizing Fluorescence Microscopy Resolutions in Biological Applications. Cells. 2023;12: pubmed publisher
Karatkevich D, Deng H, Gao Y, Flint E, Peng R, Schmid R, et al. Schedule-Dependent Treatment Increases Chemotherapy Efficacy in Malignant Pleural Mesothelioma. Int J Mol Sci. 2022;23: pubmed publisher
Furia L, Pelicci S, Scanarini M, Pelicci P, Faretta M. From Double-Strand Break Recognition to Cell-Cycle Checkpoint Activation: High Content and Resolution Image Cytometry Unmasks 53BP1 Multiple Roles in DNA Damage Response and p53 Action. Int J Mol Sci. 2022;23: pubmed publisher
Carraro C, Bonaguro L, Schulte Schrepping J, Horne A, Oestreich M, Warnat Herresthal S, et al. Decoding mechanism of action and sensitivity to drug candidates from integrated transcriptome and chromatin state. elife. 2022;11: pubmed publisher
Sulaj A, Kopf S, von Rauchhaupt E, Kliemank E, Brune M, Kender Z, et al. Six-Month Periodic Fasting in Patients With Type 2 Diabetes and Diabetic Nephropathy: A Proof-of-Concept Study. J Clin Endocrinol Metab. 2022;107:2167-2181 pubmed publisher
Lee Y, DiMaulo Milk E, Leslie J, Ding L. Hematopoietic stem cells temporally transition to thrombopoietin dependence in the fetal liver. Sci Adv. 2022;8:eabm7688 pubmed publisher
Fabián Morales E, Fernández Cáceres C, Gudiño A, Andonegui Elguera M, Torres Arciga K, Escobar Arrazola M, et al. Genotoxicity of Marijuana in Mono-Users. Front Psychiatry. 2021;12:753562 pubmed publisher
Callender L, Schroth J, Carroll E, Garrod Ketchley C, Romano L, Hendy E, et al. GATA3 induces mitochondrial biogenesis in primary human CD4+ T cells during DNA damage. Nat Commun. 2021;12:3379 pubmed publisher
Wang Z, Rein B, Zhong P, Williams J, Cao Q, Yang F, et al. Autism risk gene KMT5B deficiency in prefrontal cortex induces synaptic dysfunction and social deficits via alterations of DNA repair and gene transcription. Neuropsychopharmacology. 2021;46:1617-1626 pubmed publisher
de Groot S, Lugtenberg R, Cohen D, Welters M, Ehsan I, Vreeswijk M, et al. Fasting mimicking diet as an adjunct to neoadjuvant chemotherapy for breast cancer in the multicentre randomized phase 2 DIRECT trial. Nat Commun. 2020;11:3083 pubmed publisher
Nemcakova I, Jirka I, Doubková M, Bacakova L. Heat treatment dependent cytotoxicity of silicalite-1 films deposited on Ti-6Al-4V alloy evaluated by bone-derived cells. Sci Rep. 2020;10:9456 pubmed publisher
Stopfer L, Mesfin J, Joughin B, Lauffenburger D, White F. Multiplexed relative and absolute quantitative immunopeptidomics reveals MHC I repertoire alterations induced by CDK4/6 inhibition. Nat Commun. 2020;11:2760 pubmed publisher
Chen C, Sun M, Warzecha C, Bachu M, Dey A, Wu T, et al. HIRA, a DiGeorge Syndrome Candidate Gene, Confers Proper Chromatin Accessibility on HSCs and Supports All Stages of Hematopoiesis. Cell Rep. 2020;30:2136-2149.e4 pubmed publisher
Jiao S, Subudhi S, Aparicio A, Ge Z, Guan B, Miura Y, et al. Differences in Tumor Microenvironment Dictate T Helper Lineage Polarization and Response to Immune Checkpoint Therapy. Cell. 2019;179:1177-1190.e13 pubmed publisher
Pond K, de Renty C, Yagle M, Ellis N. Rescue of collapsed replication forks is dependent on NSMCE2 to prevent mitotic DNA damage. PLoS Genet. 2019;15:e1007942 pubmed publisher
Fichtman B, Zagairy F, Biran N, Barsheshet Y, Chervinsky E, Ben Neriah Z, et al. Combined loss of LAP1B and LAP1C results in an early onset multisystemic nuclear envelopathy. Nat Commun. 2019;10:605 pubmed publisher
Picard E, Godet Y, Laheurte C, Dosset M, Galaine J, Beziaud L, et al. Circulating NKp46+ Natural Killer cells have a potential regulatory property and predict distinct survival in Non-Small Cell Lung Cancer. Oncoimmunology. 2019;8:e1527498 pubmed publisher
Prasad C, Prasad S, Yadav S, Pandey L, Singh S, Pradhan S, et al. Olaparib modulates DNA repair efficiency, sensitizes cervical cancer cells to cisplatin and exhibits anti-metastatic property. Sci Rep. 2017;7:12876 pubmed publisher
Castro M, Carson G, McConnell M, Herst P. High Dose Ascorbate Causes Both Genotoxic and Metabolic Stress in Glioma Cells. Antioxidants (Basel). 2017;6: pubmed publisher
Kopova I, Lavrentiev V, Vacik J, Bacakova L. Growth and potential damage of human bone-derived cells cultured on fresh and aged C60/Ti films. PLoS ONE. 2015;10:e0123680 pubmed publisher
Maya Mendoza A, Ostrakova J, Kosar M, Hall A, Duskova P, Mistrik M, et al. Myc and Ras oncogenes engage different energy metabolism programs and evoke distinct patterns of oxidative and DNA replication stress. Mol Oncol. 2015;9:601-16 pubmed publisher
Horrell S, Chalker D. LIA4 encodes a chromoshadow domain protein required for genomewide DNA rearrangements in Tetrahymena thermophila. Eukaryot Cell. 2014;13:1300-11 pubmed publisher
Zhdanova N, Draskovic I, Minina J, Karamysheva T, Novo C, Liu W, et al. Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells. Mol Cell Biol. 2014;34:2786-99 pubmed publisher
Becker M, Schmezer P, Breuer R, Haas S, Essers M, Krammer P, et al. The traditional Chinese medical compound Rocaglamide protects nonmalignant primary cells from DNA damage-induced toxicity by inhibition of p53 expression. Cell Death Dis. 2014;5:e1000 pubmed publisher
Crown K, Savytskyy O, Malik S, LOGSDON J, Williams R, Tainer J, et al. A mutation in the FHA domain of Coprinus cinereus Nbs1 Leads to Spo11-independent meiotic recombination and chromosome segregation. G3 (Bethesda). 2013;3:1927-43 pubmed publisher
Furia L, Pelicci P, Faretta M. A computational platform for robotized fluorescence microscopy (II): DNA damage, replication, checkpoint activation, and cell cycle progression by high-content high-resolution multiparameter image-cytometry. Cytometry A. 2013;83:344-55 pubmed publisher
Jha H, Upadhyay S, A J Prasad M, Lu J, Cai Q, Saha A, et al. H2AX phosphorylation is important for LANA-mediated Kaposi's sarcoma-associated herpesvirus episome persistence. J Virol. 2013;87:5255-69 pubmed publisher
Titus S, Li F, Stobezki R, Akula K, Unsal E, Jeong K, et al. Impairment of BRCA1-related DNA double-strand break repair leads to ovarian aging in mice and humans. Sci Transl Med. 2013;5:172ra21 pubmed publisher
Heo J, Cho J, Kim J. HJURP regulates cellular senescence in human fibroblasts and endothelial cells via a p53-dependent pathway. J Gerontol A Biol Sci Med Sci. 2013;68:914-25 pubmed publisher
Schenkwein D, Turkki V, Ahlroth M, Timonen O, Airenne K, Yla Herttuala S. rDNA-directed integration by an HIV-1 integrase--I-PpoI fusion protein. Nucleic Acids Res. 2013;41:e61 pubmed publisher
Kuefner M, Brand M, Ehrlich J, Braga L, Uder M, Semelka R. Effect of antioxidants on X-ray-induced ?-H2AX foci in human blood lymphocytes: preliminary observations. Radiology. 2012;264:59-67 pubmed publisher
Yoshihara Y, Wu D, Kubo N, Sang M, Nakagawara A, Ozaki T. Inhibitory role of E2F-1 in the regulation of tumor suppressor p53 during DNA damage response. Biochem Biophys Res Commun. 2012;421:57-63 pubmed publisher
Zhang M, Kleber S, Röhrich M, Timke C, Han N, Tuettenberg J, et al. Blockade of TGF-? signaling by the TGF?R-I kinase inhibitor LY2109761 enhances radiation response and prolongs survival in glioblastoma. Cancer Res. 2011;71:7155-67 pubmed publisher
McGee Lawrence M, McCleary Wheeler A, Secreto F, Razidlo D, Zhang M, Stensgard B, et al. Suberoylanilide hydroxamic acid (SAHA; vorinostat) causes bone loss by inhibiting immature osteoblasts. Bone. 2011;48:1117-26 pubmed publisher
Bu Y, Suenaga Y, Okoshi R, Sang M, Kubo N, Song F, et al. NFBD1/MDC1 participates in the regulation of G2/M transition in mammalian cells. Biochem Biophys Res Commun. 2010;397:157-62 pubmed publisher
Lukaszewicz A, Howard Till R, Novatchkova M, Mochizuki K, Loidl J. MRE11 and COM1/SAE2 are required for double-strand break repair and efficient chromosome pairing during meiosis of the protist Tetrahymena. Chromosoma. 2010;119:505-18 pubmed publisher
Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S. A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks. Nucleic Acids Res. 2010;38:e129 pubmed publisher
Yamada C, Ozaki T, Ando K, Suenaga Y, Inoue K, Ito Y, et al. RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at Ser-15 and acts as a co-activator for p53. J Biol Chem. 2010;285:16693-703 pubmed publisher
Leone S, Cornetta T, Basso E, Cozzi R. Resveratrol induces DNA double-strand breaks through human topoisomerase II interaction. Cancer Lett. 2010;295:167-72 pubmed publisher
Massignan T, Stewart R, Biasini E, Solomon I, Bonetto V, Chiesa R, et al. A novel, drug-based, cellular assay for the activity of neurotoxic mutants of the prion protein. J Biol Chem. 2010;285:7752-65 pubmed publisher
Beels L, Bacher K, De Wolf D, Werbrouck J, Thierens H. gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?. Circulation. 2009;120:1903-9 pubmed publisher
Loidl J, Mochizuki K. Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Mol Biol Cell. 2009;20:2428-37 pubmed publisher
Akbay E, Contreras C, Perera S, Sullivan J, Broaddus R, Schorge J, et al. Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Am J Pathol. 2008;173:536-44 pubmed publisher
Mochizuki K, Novatchkova M, Loidl J. DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. J Cell Sci. 2008;121:2148-58 pubmed publisher
Rossi D, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman I. Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Nature. 2007;447:725-9 pubmed
Xiao R, Sun Y, Ding J, Lin S, Rose D, Rosenfeld M, et al. Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Mol Cell Biol. 2007;27:5393-402 pubmed
product information
Antigen :
H2A.X Phospho Ser139
Apps. Abbrev. :
ICFC
Cat # :
613405
Clone :
2F3
Item :
Alexa Fluor® 488 anti-H2A.X Phospho (Ser139)
Isotype :
Mouse IgG1, κ
Other Names :
H2A.x, H2a/x, Histone 2A, Histone 2A.X, Gamma-H2AX
Size :
25 tests
Price (USD) :
188 USD
Reactivity :
Human, Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A488
Immunogen :
Modified peptide
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA