product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-SSRP1
catalog :
609701
quantity :
25 μg
price :
101 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10D1
reactivity :
African green monkey, human, mouse
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
more info or order :
citations: 23
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; African green monkey; 1:200; fig 5
Dembowski J, DeLuca N. Selective recruitment of nuclear factors to productively replicating herpes simplex virus genomes. PLoS Pathog. 2015;11:e1004939 pubmed publisher
Jeong E, Martina J, Contreras P, Lee J, Puertollano R. The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3. Autophagy. 2022;18:2333-2349 pubmed publisher
Goswami I, Sandlesh P, Stablewski A, Toshkov I, Safina A, Magnitov M, et al. FACT maintains nucleosomes during transcription and stem cell viability in adult mice. EMBO Rep. 2022;23:e53684 pubmed publisher
Li X, Li H, Jing Q, Wang M, Hu T, Li L, et al. Structural insights into multifunctionality of human FACT complex subunit hSSRP1. J Biol Chem. 2021;297:101360 pubmed publisher
Isa N, Bensaude O, Aziz N, Murphy S. HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation. Vaccines (Basel). 2021;9: pubmed publisher
Sundarraj J, Taylor G, von Kriegsheim A, Pradeepa M. H3K36me3 and PSIP1/LEDGF associate with several DNA repair proteins, suggesting their role in efficient DNA repair at actively transcribing loci. Wellcome Open Res. 2017;2:83 pubmed publisher
Narain A, Bhandare P, Adhikari B, Backes S, Eilers M, Dölken L, et al. Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination. Mol Cell. 2021;81:3110-3127.e14 pubmed publisher
Wang A, Chen L, Wu R, Hao Y, McSwiggen D, Heckert A, et al. The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells. Mol Cell. 2020;79:221-233.e5 pubmed publisher
Prendergast L, Hong E, Safina A, Poe D, Gurova K. Histone chaperone FACT is essential to overcome replication stress in mammalian cells. Oncogene. 2020;39:5124-5137 pubmed publisher
Srinivasan R, Nady N, Arora N, Hsieh L, Swigut T, Narlikar G, et al. Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage. Sci Adv. 2020;6:eaaz9115 pubmed publisher
Piquet S, Le Parc F, Bai S, Chevallier O, Adam S, Polo S. The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage. Mol Cell. 2018;72:888-901.e7 pubmed publisher
Paul S, Ghosh S, Mandal S, Sau S, Pal M. NRF2 transcriptionally activates the heat shock factor 1 promoter under oxidative stress and affects survival and migration potential of MCF7 cells. J Biol Chem. 2018;293:19303-19316 pubmed publisher
Ali A, Biswas A, Pal M. HSF1 mediated TNF-α production during proteotoxic stress response pioneers proinflammatory signal in human cells. FASEB J. 2018;:fj201801482R pubmed publisher
Sandlesh P, Juang T, Safina A, Higgins M, Gurova K. Uncovering the fine print of the CreERT2-LoxP system while generating a conditional knockout mouse model of Ssrp1 gene. PLoS ONE. 2018;13:e0199785 pubmed publisher
Fleyshman D, Prendergast L, Safina A, Paszkiewicz G, Commane M, Morgan K, et al. Level of FACT defines the transcriptional landscape and aggressive phenotype of breast cancer cells. Oncotarget. 2017;8:20525-20542 pubmed publisher
Safina A, Cheney P, Pal M, Brodsky L, Ivanov A, Kirsanov K, et al. FACT is a sensor of DNA torsional stress in eukaryotic cells. Nucleic Acids Res. 2017;45:1925-1945 pubmed publisher
Dermawan J, Hitomi M, Silver D, Wu Q, Sandlesh P, Sloan A, et al. Pharmacological Targeting of the Histone Chaperone Complex FACT Preferentially Eliminates Glioblastoma Stem Cells and Prolongs Survival in Preclinical Models. Cancer Res. 2016;76:2432-42 pubmed publisher
Shakya A, Callister C, Goren A, Yosef N, Garg N, Khoddami V, et al. Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion. Mol Cell Biol. 2015;35:1014-25 pubmed publisher
Dermawan J, Gurova K, Pink J, Dowlati A, De S, Narla G, et al. Quinacrine overcomes resistance to erlotinib by inhibiting FACT, NF-?B, and cell-cycle progression in non-small cell lung cancer. Mol Cancer Ther. 2014;13:2203-14 pubmed publisher
Aida M, Hamad N, Stanlie A, Begum N, Honjo T. Accumulation of the FACT complex, as well as histone H3.3, serves as a target marker for somatic hypermutation. Proc Natl Acad Sci U S A. 2013;110:7784-9 pubmed publisher
Lolis A, Londhe P, Beggs B, Byrum S, Tackett A, Davie J. Myogenin recruits the histone chaperone facilitates chromatin transcription (FACT) to promote nucleosome disassembly at muscle-specific genes. J Biol Chem. 2013;288:7676-87 pubmed publisher
Carvalho S, Raposo A, Martins F, Grosso A, Sridhara S, Rino J, et al. Histone methyltransferase SETD2 coordinates FACT recruitment with nucleosome dynamics during transcription. Nucleic Acids Res. 2013;41:2881-93 pubmed publisher
De Wever V, Lloyd D, Nasa I, Nimick M, Trinkle Mulcahy L, Gourlay R, et al. Isolation of human mitotic protein phosphatase complexes: identification of a complex between protein phosphatase 1 and the RNA helicase Ddx21. PLoS ONE. 2012;7:e39510 pubmed publisher
product information
Antigen :
SSRP1
Apps. Abbrev. :
WB, ICC, IP, ChIP
Cat # :
609701
Clone :
10D1
Item :
Purified anti-SSRP1
Isotype :
Mouse IgG2b, κ
Other Names :
Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein
Size :
25 μg
Price (USD) :
101 USD
Reactivity :
Human, Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Recombinant (partial) (308-524aa)
Application Notes :
10D1 displayed a stronger affinity for SSRP1 than BioLegend clone 3E4 when tested for western blot application.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA