product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-Spt16 (FACT140 complex)
catalog :
607002
quantity :
100 μg
price :
218 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8D2
reactivity :
human, mouse
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq
more info or order :
citations: 13
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 1a
Safina A, Cheney P, Pal M, Brodsky L, Ivanov A, Kirsanov K, et al. FACT is a sensor of DNA torsional stress in eukaryotic cells. Nucleic Acids Res. 2017;45:1925-1945 pubmed publisher
  • ChIP-Seq; human; fig 4
  • chromatin immunoprecipitation; human; fig 3
Shakya A, Callister C, Goren A, Yosef N, Garg N, Khoddami V, et al. Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion. Mol Cell Biol. 2015;35:1014-25 pubmed publisher
Goswami I, Sandlesh P, Stablewski A, Toshkov I, Safina A, Magnitov M, et al. FACT maintains nucleosomes during transcription and stem cell viability in adult mice. EMBO Rep. 2022;23:e53684 pubmed publisher
Isa N, Bensaude O, Aziz N, Murphy S. HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation. Vaccines (Basel). 2021;9: pubmed publisher
Kaja A, Adhikari A, Karmakar S, Zhang W, Rothschild G, Basu U, et al. Proteasomal Regulation of Mammalian SPT16 in Controlling Transcription. Mol Cell Biol. 2021;41: pubmed publisher
Prendergast L, Hong E, Safina A, Poe D, Gurova K. Histone chaperone FACT is essential to overcome replication stress in mammalian cells. Oncogene. 2020;39:5124-5137 pubmed publisher
Hu X, Zheng W, Zhu Q, Gu L, Du Y, Han Z, et al. Increase in DNA Damage by MYCN Knockdown Through Regulating Nucleosome Organization and Chromatin State in Neuroblastoma. Front Genet. 2019;10:684 pubmed publisher
Sandlesh P, Juang T, Safina A, Higgins M, Gurova K. Uncovering the fine print of the CreERT2-LoxP system while generating a conditional knockout mouse model of Ssrp1 gene. PLoS ONE. 2018;13:e0199785 pubmed publisher
Lin D, Hiron T, O Callaghan C. Intragenic transcriptional interference regulates the human immune ligand MICA. EMBO J. 2018;37: pubmed publisher
Fleyshman D, Prendergast L, Safina A, Paszkiewicz G, Commane M, Morgan K, et al. Level of FACT defines the transcriptional landscape and aggressive phenotype of breast cancer cells. Oncotarget. 2017;8:20525-20542 pubmed publisher
Burmistrova D, Tillib S, Shcheblyakov D, Dolzhikova I, Shcherbinin D, Zubkova O, et al. Genetic Passive Immunization with Adenoviral Vector Expressing Chimeric Nanobody-Fc Molecules as Therapy for Genital Infection Caused by Mycoplasma hominis. PLoS ONE. 2016;11:e0150958 pubmed publisher
Lolis A, Londhe P, Beggs B, Byrum S, Tackett A, Davie J. Myogenin recruits the histone chaperone facilitates chromatin transcription (FACT) to promote nucleosome disassembly at muscle-specific genes. J Biol Chem. 2013;288:7676-87 pubmed publisher
Adelmant G, Calkins A, Garg B, Card J, Askenazi M, Miron A, et al. DNA ends alter the molecular composition and localization of Ku multicomponent complexes. Mol Cell Proteomics. 2012;11:411-21 pubmed publisher
product information
Antigen :
Spt16 FACT140 complex
Apps. Abbrev. :
WB, ICC, IP, ChIP
Cat # :
607002
Clone :
8D2
Item :
Purified anti-Spt16 (FACT140 complex)
Isotype :
Mouse IgG2a, κ
Other Names :
Facilitates Chromatin Transcription (FACT140), Spt 16 protein, Cdc68
Size :
100 μg
Price (USD) :
218 USD
Reactivity :
Human, Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Recombinant (full length)
Application Notes :
25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution. ICC positive in HeLa Cells.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA