Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Purified anti-PIEZO1

602752

100 μg

293 USD

monoclonal

rat

nonconjugated

W17241A

human, mouse

western blot, immunohistochemistry - paraffin section

IHC-P, WB

602752

W17241A

Purified anti-PIEZO1

Rat IgG2b, κ

PIEZO Type Mechanosensitive Ion Channel Component 1, Membrane Protein Induced By Beta-Amyloid Treatment, FAM38A, Mib, KIAA023, LMPH3, DHS

100 μg

293 USD

Human, Mouse

Monoclonal

Rat

PURE

Partial recombinant human PIEZO1 protein

When testing this clone for western blot, we observed a considerable loss of signal with lysates that were boiled in sample buffer compared to samples that were heated to 37°C. This is likely due to high temperatures causing aggregation of PIEZO1, which is a multi-pass transmembrane domain protein.This clone was tested for immunocytochemistry on HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in high non-specific staining.;