Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Purified anti-Lck

601001

25 μg

101 USD

monoclonal

mouse

nonconjugated

A17013D

human, mouse

western blot, immunocytochemistry, immunoprecipitation

WB, ICC, IP

601001

A17013D

Purified anti-Lck

Mouse IgG1, κ

Proto-oncogene tyrosine kinase lck, p56-Lck, T-cell specific protein tyrosine kinase

25 μg

101 USD

Human, Mouse

Monoclonal

Mouse

PURE

Human Lck peptide phosphorylated around Tyr505.

Clone A17013D was derived from a human Lck peptide phosphorylated at Y505 immunogen, but still displayed pan reactivity towards the unmodified peptide during western blot screening. This clone was moderately stronger than BioLegend clone LCK-01 for western blot and substantially stronger for immunofluorescence microscopy in parallel testing. But it shows a weak affinity towards mouse Lck. This clone is predicted to recognize both 58 and 61 kD isoforms based off the presence of the immunizing epitope in both proteins and may also recognize certain SRC tyrosine kinase family members due to high sequence similarity.Phosphorylation of Lck Y505 reduces binding of this antibody by western blot (please see Fig. 3); we recommend LCK-01 as a pan control antibody.The additional band of about 50 kD detected bellow the canonical Lck band in IP experiment could be Lck degradation product.;