Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Purified anti-AMPKα Phospho (Thr172)

600652

100 μg

329 USD

monoclonal

mouse

nonconjugated

A20017A

human, mouse

western blot, immunocytochemistry, immunohistochemistry - paraffin section

WB, ICC, ICFC, IHC-P

600652

A20017A

Purified anti-AMPKα Phospho (Thr172)

Mouse IgG1, κ

PRKAA1, PRKAA2, AMPKa1, AMPKa2, AMPK, ACACA Kinase, Tau-Protein Kinase PRKAA1, Tau-Protein Kinase PRKAA2, Protein kinase AMP-Activated, Alpha 1 Catalytic Subunit, HMGCR Kinase

100 μg

329 USD

Human, Mouse

Monoclonal

Mouse

PURE

Synthetic peptide of human AMPK alpha phosphorylated at Thr172

This clone was tested for ICC using serum-starved untreated HEK293 cells (negative control) and HEK293 cells treated with 5.0 µM oligomycin for 30 minutes (positive control). Staining was expected to localize to the nucleus and cytoplasm. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Both PFA fixation followed by methanol permeabilization and PFA fixation followed by Triton X-100 permeabilization produced a strong signal with the expected localization. Methanol-only fixation only produced a faint signal.;