Recombinant Human Cathepsin E (carrier-free) | BioLegend 599304 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

protein

product name :

Recombinant Human Cathepsin E (carrier-free)

catalog :

599304

quantity :

25 μg

price :

395 USD

citations: 2

Reference
Weisberg S, Carpenter D, Chait M, Dogra P, Gartrell Corrado R, Chen A, et al. Tissue-Resident Memory T Cells Mediate Immune Homeostasis in the Human Pancreas through the PD-1/PD-L1 Pathway. Cell Rep. 2019;29:3916-3932.e5 pubmed publisher
Solimani F, Pollmann R, Schmidt T, Schmidt A, Zheng X, Savai R, et al. Therapeutic Targeting of Th17/Tc17 Cells Leads to Clinical Improvement of Lichen Planus. Front Immunol. 2019;10:1808 pubmed publisher

product information

Antigen :

Cathepsin E

Apps. Abbrev. :

Cat # :

599304

Item :

Recombinant Human Cathepsin E (carrier-free)

Other Names :

CE, CATE

Size :

25 μg

Price (USD) :

395 USD

Reactivity :

Human

Host :

293E cells

Conjugate/Tag/Label :

RECOM

Application Notes :

Activation Protocol Step 1: Dilute recombinant human CTSE to 1 ug/ml in assay buffer (0.1 M NaOAc, 0.5 M NaCl, pH 3.5) and incubate the protein at 25 C for 30 min to activate CTSE. After activation, dilute activated CTSE to 0.2 ug/ml using assay buffer. Step 2: Dilute fluorogenic peptide substrate (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) to 40 uM using assay buffer. Step 3: Perform the assay in a 96-well, black, flat-bottomed plate. Add 50 ul of the diluted, activated CTSE (0.2 ug/ml) to a black well and start the reaction by adding 50 ul of 40 nm substrate. Assay is read on a fluorometer at excitation and emission wavelengths of 320 nm and 405 nm (top read). Note: This protein is in the latent form and needs to be activated for bioassay. . BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee . If you have any concerns, contact us at tech@biolegend.com .