Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human Cathepsin E (carrier-free)

599302

10 μg

229 USD

BA

599302

Recombinant Human Cathepsin E (carrier-free)

CE, CATE

10 μg

229 USD

Human

ADD EXPRESSION SYSTEM HERE

RECOM

Activation Protocol ;Step 1: Dilute recombinant human CTSE to 1 μg/ml in assay buffer (0.1 M NaOAc, 0.5 M NaCl, pH 3.5) and incubate the protein at 25°C for 30 min to activate CTSE. After activation, dilute activated CTSE to 0.2 μg/ml using assay buffer.Step 2: Dilute fluorogenic peptide substrate (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) to 40 μM using assay buffer.Step 3: Perform the assay in a 96-well, black, flat-bottomed plate. Add 50 μl of the diluted, activated CTSE (0.2 μg/ml) to a black well and start the reaction by adding 50 μl of 40 nm substrate. Assay is read on a fluorometer at excitation and emission wavelengths of 320 nm and 405 nm (top read).Note: This protein is in the latent form and needs to be activated for bioassay.;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;