Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human Cathepsin D (carrier-free)

556706

100 μg

1167 USD

BA

556706

Recombinant Human Cathepsin D (carrier-free)

CTSD

100 μg

1167 USD

Human

ADD EXPRESSION SYSTEM HERE

RECOM

Step 1: Dilute recombinant human CTSD to 20 µg/ml in assay buffer (0.1 M NaOAc, 0.2 M NaCl, pH 3.5) and incubate the protein at 37°C for 30 min to activate CTSD. After activation, dilute the activated CTSD to 1 ng/µL using assay buffer.Step 2: Dilute fluorogenic peptide substrate (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) to 20 µM using assay buffer.Step 3: Perform the assay in a 96-well, black, flat-bottom plate. Add 50 µL of the diluted, activated CTSD to a black well and start the reaction by adding 50 µL of the diluted substrate. Assay is read on a fluorometer at excitation and emission wavelengths of 320 nM and 405 nM (top read). This protein is in the latent form and needs to be activated for bioassay.; BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;