Recombinant Human Cathepsin D (carrier-free) | BioLegend 556702 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

protein

product name :

Recombinant Human Cathepsin D (carrier-free)

catalog :

556702

quantity :

10 μg

price :

190 USD

citations: 1

Reference
Brown C, Gudjonson H, Pritykin Y, Deep D, Lavallée V, Mendoza A, et al. Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity. Cell. 2019;179:846-863.e24 pubmed publisher

product information

Antigen :

Cathepsin D

Apps. Abbrev. :

Cat # :

556702

Item :

Recombinant Human Cathepsin D (carrier-free)

Other Names :

CTSD

Size :

10 μg

Price (USD) :

190 USD

Reactivity :

Human

Host :

293E cells

Conjugate/Tag/Label :

RECOM

Application Notes :

Step 1: Dilute recombinant human CTSD to 20 ug/ml in assay buffer (0.1 M NaOAc, 0.2 M NaCl, pH 3.5) and incubate the protein at 37 C for 30 min to activate CTSD. After activation, dilute the activated CTSD to 1 ng/uL using assay buffer. Step 2: Dilute fluorogenic peptide substrate (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) to 20 uM using assay buffer. Step 3: Perform the assay in a 96-well, black, flat-bottom plate. Add 50 uL of the diluted, activated CTSD to a black well and start the reaction by adding 50 uL of the diluted substrate. Assay is read on a fluorometer at excitation and emission wavelengths of 320 nM and 405 nM (top read). . This protein is in the latent form and needs to be activated for bioassay. . . BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee . If you have any concerns, contact us at tech@biolegend.com .