Alexa Fluor® 647 anti-mouse IL-22 | BioLegend 516406 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Alexa Fluor® 647 anti-mouse IL-22

catalog :

516406

quantity :

100 tests

price :

176 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

AF647

clone name :

Poly5164

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 3b	Kong W, Tsuyama N, Inoue H, Guo Y, Mokuda S, Nobukiyo A, et al. Long-chain saturated fatty acids in breast milk are associated with the pathogenesis of atopic dermatitis via induction of inflammatory ILC3s. Sci Rep. 2021;11:13109 pubmed publisher
flow cytometry; mouse; loading ...; fig s3a	Tripathi D, Venkatasubramanian S, Cheekatla S, Paidipally P, Welch E, Tvinnereim A, et al. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice. Nat Commun. 2016;7:13896 pubmed publisher
	Qi X, Yun C, Sun L, Xia J, Wu Q, Wang Y, et al. Gut microbiota-bile acid-interleukin-22 axis orchestrates polycystic ovary syndrome. Nat Med. 2019;25:1225-1233 pubmed publisher
	Wang T, Fan C, Yao A, Xu X, Zheng G, You Y, et al. The Adaptor Protein CARD9 Protects against Colon Cancer by Restricting Mycobiota-Mediated Expansion of Myeloid-Derived Suppressor Cells. Immunity. 2018;49:504-514.e4 pubmed publisher
	Engdahl C, Bondt A, Harre U, Raufer J, Pfeifle R, Camponeschi A, et al. Estrogen induces St6gal1 expression and increases IgG sialylation in mice and patients with rheumatoid arthritis: a potential explanation for the increased risk of rheumatoid arthritis in postmenopausal women. Arthritis Res Ther. 2018;20:84 pubmed publisher
	Rafei Shamsabadi D, van de Poel S, Dorn B, Kunz S, Martin S, Klose C, et al. Lack of Type 2 Innate Lymphoid Cells Promotes a Type I-Driven Enhanced Immune Response in Contact Hypersensitivity. J Invest Dermatol. 2018;138:1962-1972 pubmed publisher
	Emgård J, Kammoun H, García Cassani B, Chesne J, Parigi S, Jacob J, et al. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. Immunity. 2018;48:120-132.e8 pubmed publisher
	Campisi L, Barbet G, Ding Y, Esplugues E, Flavell R, Blander J. Apoptosis in response to microbial infection induces autoreactive TH17 cells. Nat Immunol. 2016;17:1084-92 pubmed publisher
	Alves C, Ober BlÃ¶baum J, Brouwers Haspels I, Asmawidjaja P, Mus A, Razawy W, et al. Dendritic Cell-Specific Deletion of Î²-Catenin Results in Fewer Regulatory T-Cells without Exacerbating Autoimmune Collagen-Induced Arthritis. PLoS ONE. 2015;10:e0142972 pubmed publisher

product information

Antigen :

IL-22

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

516406

Clone :

Poly5164

Item :

Alexa Fluor® 647 anti-mouse IL-22

Isotype :

Goat Polyclonal IgG

Other Names :

Interleukin-22, IL-10-related T cell-derived inducible factor, IL-TIF

Size :

100 tests

Price (USD) :

176 USD

Reactivity :

Mouse

Clonality :

Polyclonal

Host :

Goat

Conjugate/Tag/Label :

A647

Immunogen :

Mouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed in E. coli .

Application Notes :

ELISA Capture: The purified Poly5164 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5164 antibody (Cat. No. 516407) as the detecting antibody and recombinant mouse IL-22 (Cat. No. 576209) as the standard. In sandwich ELISA, Poly5164 has approximately 17% and 2% cross-reactivity with recombinant rat and human IL-22, respectively. Flow Cytometry: The fluorochrome-labeled Poly5164 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify IL-22-producing cells within mixed cell populations. Note: For testing mouse IL-22 in serum, plasma or supernatant, LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307 436308) is specially developed and recommended. Note: The use of Iscove s Modified Dulbecco s Medium (IMDM) will result in better in vitro Th17 polarization. 1

more info or order :

BioLegend product webpage