PE anti-mouse IL-22 | BioLegend 516404 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE anti-mouse IL-22

catalog :

516404

quantity :

100 tests

price :

150 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

clone name :

Poly5164

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s3a	Tripathi D, Venkatasubramanian S, Cheekatla S, Paidipally P, Welch E, Tvinnereim A, et al. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice. Nat Commun. 2016;7:13896 pubmed publisher
	Martínez López M, Iborra S, Conde Garrosa R, Mastrangelo A, Danne C, Mann E, et al. Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity. Immunity. 2019;50:446-461.e9 pubmed publisher
	Miani M, Le Naour J, Waeckel Enée E, Verma S, Straube M, Emond P, et al. Gut Microbiota-Stimulated Innate Lymphoid Cells Support ?-Defensin 14 Expression in Pancreatic Endocrine Cells, Preventing Autoimmune Diabetes. Cell Metab. 2018;: pubmed publisher
	Puerta Arias J, Pino Tamayo P, Arango J, Gonzalez A. Depletion of Neutrophils Promotes the Resolution of Pulmonary Inflammation and Fibrosis in Mice Infected with Paracoccidioides brasiliensis. PLoS ONE. 2016;11:e0163985 pubmed publisher
	Barone F, Nayar S, Campos J, Cloake T, Withers D, Toellner K, et al. IL-22 regulates lymphoid chemokine production and assembly of tertiary lymphoid organs. Proc Natl Acad Sci U S A. 2015;112:11024-9 pubmed publisher
	Zhao X, Zhu D, Ye J, Li X, Wang Z, Zhang L, et al. The potential protective role of the combination of IL-22 and TNF-Î± against genital tract Chlamydia trachomatis infection. Cytokine. 2015;73:66-73 pubmed publisher

product information

Antigen :

IL-22

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

516404

Clone :

Poly5164

Item :

PE anti-mouse IL-22

Isotype :

Goat Polyclonal IgG

Other Names :

Interleukin-22, IL-10-related T cell-derived inducible factor, IL-TIF

Size :

100 tests

Price (USD) :

150 USD

Reactivity :

Mouse

Clonality :

Polyclonal

Host :

Goat

Conjugate/Tag/Label :

Immunogen :

Mouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed in E. coli .

Application Notes :

ELISA Capture: The purified Poly5164 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5164 antibody (Cat. No. 516407) as the detecting antibody and recombinant mouse IL-22 (Cat. No. 576209) as the standard. In sandwich ELISA, Poly5164 has approximately 17% and 2% cross-reactivity with recombinant rat and human IL-22, respectively. Flow Cytometry: The fluorochrome-labeled Poly5164 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify IL-22-producing cells within mixed cell populations. Note: For testing mouse IL-22 in serum, plasma or supernatant, LEGEND MAX Mouse IL-22 ELISA Kit (Cat. No. 436307 436308) is specially developed and recommended. Note: The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization. 1

more info or order :

BioLegend product webpage