product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-human/mouse Granzyme B
catalog :
515405
quantity :
25 tests
price :
171 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF647
clone name :
GB11
reactivity :
human, mouse, rat
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 111
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 6d, s4a
Hou X, Shi Y, Kang X, Rousu Z, Li D, Wang M, et al. Echinococcus granulosus: The establishment of the metacestode in the liver is associated with control of the CD4+ T-cell-mediated immune response in patients with cystic echinococcosis and a mouse model. Front Cell Infect Microbiol. 2022;12:983119 pubmed publisher
  • flow cytometry; mouse; fig 3f
Wu B, Song M, Dong Q, Xiang G, Li J, Ma X, et al. UBR5 promotes tumor immune evasion through enhancing IFN-γ-induced PDL1 transcription in triple negative breast cancer. Theranostics. 2022;12:5086-5102 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3f
Pan C, Wu Q, Wang S, Mei Z, Zhang L, Gao X, et al. Combination with Toll-like receptor 4 (TLR4) agonist reverses GITR agonism mediated M2 polarization of macrophage in Hepatocellular carcinoma. Oncoimmunology. 2022;11:2073010 pubmed publisher
  • flow cytometry; human; fig s10
Kono M, Komatsuda H, Yamaki H, Kumai T, Hayashi R, Wakisaka R, et al. Immunomodulation via FGFR inhibition augments FGFR1 targeting T-cell based antitumor immunotherapy for head and neck squamous cell carcinoma. Oncoimmunology. 2022;11:2021619 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5b
Susukida T, Kuwahara S, Song B, Kazaoka A, Aoki S, Ito K. Regulation of the immune tolerance system determines the susceptibility to HLA-mediated abacavir-induced skin toxicity. Commun Biol. 2021;4:1137 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s4b
Souza C, Ketelut Carneiro N, Milanezi C, Faccioli L, Gardinassi L, Silva J. NLRC4 inhibits NLRP3 inflammasome and abrogates effective antifungal CD8+ T cell responses. iScience. 2021;24:102548 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 3d
Lu M, Dravid P, Zhang Y, Trivedi S, Li A, Harder O, et al. A safe and highly efficacious measles virus-based vaccine expressing SARS-CoV-2 stabilized prefusion spike. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5f, 5g
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d, 2l
Leone R, Zhao L, Englert J, Sun I, Oh M, Sun I, et al. Glutamine blockade induces divergent metabolic programs to overcome tumor immune evasion. Science. 2019;366:1013-1021 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Meckiff B, Ladell K, McLaren J, Ryan G, Leese A, James E, et al. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. 2019;203:1276-1287 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Kim A, Han C, Driver I, Olow A, Sewell A, Zhang Z, et al. LILRB1 Blockade Enhances Bispecific T Cell Engager Antibody-Induced Tumor Cell Killing by Effector CD8+ T Cells. J Immunol. 2019;203:1076-1087 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig 3h
Spolski R, West E, Li P, Veenbergen S, Yung S, Kazemian M, et al. IL-21/type I interferon interplay regulates neutrophil-dependent innate immune responses to Staphylococcus aureus. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
  • flow cytometry; human; loading ...; fig s6a
Galperin M, Farenc C, Mukhopadhyay M, Jayasinghe D, Decroos A, Benati D, et al. CD4+ T cell-mediated HLA class II cross-restriction in HIV controllers. Sci Immunol. 2018;3: pubmed publisher
  • flow cytometry; human; loading ...; fig 4c
Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
  • flow cytometry; human; loading ...; fig 6a
Djaoud Z, Guethlein L, Horowitz A, Azzi T, Nemat Gorgani N, Olive D, et al. Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells. J Exp Med. 2017;214:1827-1841 pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Srivastava R, Khan A, Garg S, Syed S, Furness J, Vahed H, et al. Human Asymptomatic Epitopes Identified from the Herpes Simplex Virus Tegument Protein VP13/14 (UL47) Preferentially Recall Polyfunctional Effector Memory CD44high CD62Llow CD8+ TEM Cells and Protect Humanized HLA-A*02:01 Transgenic Mice against Ocula. J Virol. 2017;91: pubmed publisher
  • flow cytometry; human; fig 5
Pachnio A, Ciáurriz M, Begum J, Lal N, Zuo J, Beggs A, et al. Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium. PLoS Pathog. 2016;12:e1005832 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Roan F, Stoklasek T, Whalen E, Molitor J, Bluestone J, Buckner J, et al. CD4+ Group 1 Innate Lymphoid Cells (ILC) Form a Functionally Distinct ILC Subset That Is Increased in Systemic Sclerosis. J Immunol. 2016;196:2051-2062 pubmed publisher
  • flow cytometry; mouse
Rao E, Zhang Y, Zhu G, Hao J, Persson X, Egilmez N, et al. Deficiency of AMPK in CD8+ T cells suppresses their anti-tumor function by inducing protein phosphatase-mediated cell death. Oncotarget. 2015;6:7944-58 pubmed
  • flow cytometry; mouse; fig 7
  • flow cytometry; human; fig 4
Srivastava R, Khan A, Spencer D, Vahed H, Lopes P, Thai N, et al. HLA-A02:01-restricted epitopes identified from the herpes simplex virus tegument protein VP11/12 preferentially recall polyfunctional effector memory CD8+ T cells from seropositive asymptomatic individuals and protect humanized HLA-A*02:01 transgenic. J Immunol. 2015;194:2232-48 pubmed publisher
  • flow cytometry; human; fig 4
Khan A, Srivastava R, Spencer D, Garg S, Fremgen D, Vahed H, et al. Phenotypic and functional characterization of herpes simplex virus glycoprotein B epitope-specific effector and memory CD8+ T cells from symptomatic and asymptomatic individuals with ocular herpes. J Virol. 2015;89:3776-92 pubmed publisher
  • flow cytometry; mouse; fig 6
Kobayashi T, Hamaguchi Y, Hasegawa M, Fujimoto M, Takehara K, Matsushita T. B cells promote tumor immunity against B16F10 melanoma. Am J Pathol. 2014;184:3120-9 pubmed publisher
Corria Osorio J, Carmona S, Stefanidis E, Andreatta M, Ortiz Miranda Y, Muller T, et al. Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8+ T cells. Nat Immunol. 2023;24:869-883 pubmed publisher
Oh G, Wang A, Wang L, Li J, Werba G, Weissinger D, et al. POLQ inhibition elicits an immune response in homologous recombination-deficient pancreatic adenocarcinoma via cGAS/STING signaling. J Clin Invest. 2023;133: pubmed publisher
Jeremiah N, Ferran H, Antoniadou K, De Azevedo K, Nikolic J, Maurin M, et al. RELA tunes innate-like interferon I/III responses in human T cells. J Exp Med. 2023;220: pubmed publisher
Wu Z, Thierry K, Bachy S, Zhang X, Gamradt P, Hernandez Vargas H, et al. Pericyte stem cells induce Ly6G+ cell accumulation and immunotherapy resistance in pancreatic cancer. EMBO Rep. 2023;24:e56524 pubmed publisher
Lin Y, Duong H, Limary A, Kim E, Hsu P, Patel S, et al. Small intestine and colon tissue-resident memory CD8+ T cells exhibit molecular heterogeneity and differential dependence on Eomes. Immunity. 2023;56:207-223.e8 pubmed publisher
Alkasalias T, Zhang J, Madapura H, Dalarun B, Reina O, Lewensohn R, et al. Proof-of-principle studies on a strategy to enhance nucleotide imbalance specifically in cancer cells. Cell Death Discov. 2022;8:464 pubmed publisher
Jeon Y, Kang H, Yang Y, Park D, Choi B, Kim J, et al. A Novel Selective Axl/Mer/CSF1R Kinase Inhibitor as a Cancer Immunotherapeutic Agent Targeting Both Immune and Tumor Cells in the Tumor Microenvironment. Cancers (Basel). 2022;14: pubmed publisher
Pantelidou C, Jadhav H, Kothari A, Liu R, Wulf G, Guerriero J, et al. STING agonism enhances anti-tumor immune responses and therapeutic efficacy of PARP inhibition in BRCA-associated breast cancer. NPJ Breast Cancer. 2022;8:102 pubmed publisher
Wang X, Wang K, Yu M, Velluto D, Hong X, Wang B, et al. Engineered immunomodulatory accessory cells improve experimental allogeneic islet transplantation without immunosuppression. Sci Adv. 2022;8:eabn0071 pubmed publisher
Mishra G, Jha A, Ahad A, Sen K, Sen A, Podder S, et al. Epigenomics of conventional type-I dendritic cells depicted preferential control of TLR9 versus TLR3 response by NCoR1 through differential IRF3 activation. Cell Mol Life Sci. 2022;79:429 pubmed publisher
Guo A, Huang H, Zhu Z, Chen M, Shi H, Yuan S, et al. cBAF complex components and MYC cooperate early in CD8+ T cell fate. Nature. 2022;607:135-141 pubmed publisher
Jaiswal A, Verma A, Dannenfelser R, Melssen M, Tirosh I, Izar B, et al. An activation to memory differentiation trajectory of tumor-infiltrating lymphocytes informs metastatic melanoma outcomes. Cancer Cell. 2022;40:524-544.e5 pubmed publisher
Saxena V, Piao W, Li L, Paluskievicz C, Xiong Y, Simon T, et al. Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses. Cell Rep. 2022;39:110727 pubmed publisher
Zhang T, Wang Y, Li Q, Lin L, Xu C, Xue Y, et al. Mesenchymal stromal cells equipped by IFNα empower T cells with potent anti-tumor immunity. Oncogene. 2022;41:1866-1881 pubmed publisher
Lee H, Manangeeswaran M, Lewkowicz A, Engel K, Chowdhury M, Garige M, et al. NK cells require immune checkpoint receptor LILRB4/gp49B to control neurotropic Zika virus infections in mice. JCI Insight. 2022;7: pubmed publisher
Ren C, Li M, Zheng Y, Cai B, Du W, Zhang H, et al. Single-cell RNA-seq reveals altered NK cell subsets and reduced levels of cytotoxic molecules in patients with ankylosing spondylitis. J Cell Mol Med. 2022;26:1071-1082 pubmed publisher
Wiede F, Lu K, Du X, Zeissig M, Xu R, Goh P, et al. PTP1B Is an Intracellular Checkpoint that Limits T-cell and CAR T-cell Antitumor Immunity. Cancer Discov. 2022;12:752-773 pubmed publisher
Chen H, Smith M, Herz J, Li T, Hasley R, Le Saout C, et al. The role of protease-activated receptor 1 signaling in CD8 T cell effector functions. iScience. 2021;24:103387 pubmed publisher
Chen M, Li Y, Wu Y, Xie S, Ma J, Yue J, et al. Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer. Cancers (Basel). 2021;13: pubmed publisher
Liu W, Gao M, Li L, Chen Y, Fan H, Cai Q, et al. Homeoprotein SIX1 compromises antitumor immunity through TGF-β-mediated regulation of collagens. Cell Mol Immunol. 2021;18:2660-2672 pubmed publisher
Fang F, Cao W, Zhu W, Lam N, Li L, Gaddam S, et al. The cell-surface 5'-nucleotidase CD73 defines a functional T memory cell subset that declines with age. Cell Rep. 2021;37:109981 pubmed publisher
Xian S, Dosset M, Almanza G, Searles S, Sahani P, Waller T, et al. The unfolded protein response links tumor aneuploidy to local immune dysregulation. EMBO Rep. 2021;22:e52509 pubmed publisher
Coleby R, Lucchesi D, Pontarini E, Pitzalis C, Bombardieri M. Stepwise changes in the murine salivary gland immune response during virally-induced ectopic lymphoid structure formation. Clin Exp Rheumatol. 2021;39 Suppl 133:39-48 pubmed publisher
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Lou Y, Wang J, Peng P, Wang S, Liu P, Xu L. Downregulated TNF-α Levels after Cryo-Thermal Therapy Drive Tregs Fragility to Promote Long-Term Antitumor Immunity. Int J Mol Sci. 2021;22: pubmed publisher
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Wang B, Wang Y, Sun X, Deng G, Huang W, Wu X, et al. CXCR6 is required for antitumor efficacy of intratumoral CD8+ T cell. J Immunother Cancer. 2021;9: pubmed publisher
Lal J, Townsend M, Mehta A, Oliwa M, Miller E, Sotayo A, et al. Comparing syngeneic and autochthonous models of breast cancer to identify tumor immune components that correlate with response to immunotherapy in breast cancer. Breast Cancer Res. 2021;23:83 pubmed publisher
Di Pilato M, Kfuri Rubens R, Pruessmann J, Ozga A, Messemaker M, Cadilha B, et al. CXCR6 positions cytotoxic T cells to receive critical survival signals in the tumor microenvironment. Cell. 2021;: pubmed publisher
Pardy R, Valbon S, Cordeiro B, Krawczyk C, Richer M. An epidemic Zika virus isolate suppresses antiviral immunity by disrupting antigen presentation pathways. Nat Commun. 2021;12:4051 pubmed publisher
Kim D, Wu Y, Li Q, Oh Y. Nanoparticle-Mediated Lipid Metabolic Reprogramming of T Cells in Tumor Microenvironments for Immunometabolic Therapy. Nanomicro Lett. 2021;13:31 pubmed publisher
Liu X, Ye N, Liu S, Guan J, Deng Q, Zhang Z, et al. Hyperbaric Oxygen Boosts PD-1 Antibody Delivery and T Cell Infiltration for Augmented Immune Responses Against Solid Tumors. Adv Sci (Weinh). 2021;8:e2100233 pubmed publisher
Khuzwayo S, Mthembu M, Meermeier E, Prakadan S, Kazer S, Bassett T, et al. MR1-Restricted MAIT Cells From The Human Lung Mucosal Surface Have Distinct Phenotypic, Functional, and Transcriptomic Features That Are Preserved in HIV Infection. Front Immunol. 2021;12:631410 pubmed publisher
Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
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Huang H, Zhou P, Wei J, Long L, Shi H, Dhungana Y, et al. In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions. Cell. 2021;184:1245-1261.e21 pubmed publisher
Mathewson N, Ashenberg O, Tirosh I, Gritsch S, Perez E, Marx S, et al. Inhibitory CD161 receptor identified in glioma-infiltrating T cells by single-cell analysis. Cell. 2021;184:1281-1298.e26 pubmed publisher
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Pais Ferreira D, Silva J, Wyss T, Fuertes Marraco S, Scarpellino L, Charmoy M, et al. Central memory CD8+ T cells derive from stem-like Tcf7hi effector cells in the absence of cytotoxic differentiation. Immunity. 2020;53:985-1000.e11 pubmed publisher
Rydyznski Moderbacher C, Ramirez S, Dan J, Grifoni A, Hastie K, Weiskopf D, et al. Antigen-Specific Adaptive Immunity to SARS-CoV-2 in Acute COVID-19 and Associations with Age and Disease Severity. Cell. 2020;183:996-1012.e19 pubmed publisher
Ping Y, Li F, Nan S, Zhang D, Shi X, Shan J, et al. Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFv. Front Cell Dev Biol. 2020;8:803 pubmed publisher
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Jankeel A, Menicucci A, Woolsey C, Fenton K, Mendoza N, Versteeg K, et al. Early Transcriptional Changes within Liver, Adrenal Gland, and Lymphoid Tissues Significantly Contribute to Ebola Virus Pathogenesis in Cynomolgus Macaques. J Virol. 2020;94: pubmed publisher
Peng W, Hong Z, Chen X, Gao H, Dai Z, Zhao J, et al. Thiostrepton Reactivates Latent HIV-1 through the p-TEFb and NF-κB Pathways Mediated by Heat Shock Response. Antimicrob Agents Chemother. 2020;64: pubmed publisher
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Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
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Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
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Iwata T, Ishii C, Ishida S, Ogitani Y, Wada T, Agatsuma T. A HER2-Targeting Antibody-Drug Conjugate, Trastuzumab Deruxtecan (DS-8201a), Enhances Antitumor Immunity in a Mouse Model. Mol Cancer Ther. 2018;17:1494-1503 pubmed publisher
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de Mingo Pulido Á, Gardner A, Hiebler S, Soliman H, Rugo H, Krummel M, et al. TIM-3 Regulates CD103+ Dendritic Cell Function and Response to Chemotherapy in Breast Cancer. Cancer Cell. 2018;33:60-74.e6 pubmed publisher
Zhang Y, Li N, Suh H, Irvine D. Nanoparticle anchoring targets immune agonists to tumors enabling anti-cancer immunity without systemic toxicity. Nat Commun. 2018;9:6 pubmed publisher
Wu J, Zhang H, Shi X, Xiao X, Fan Y, Minze L, et al. Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4+ T Cell Dysfunction. Immunity. 2017;47:1114-1128.e6 pubmed publisher
Palazon A, Tyrakis P, Macías D, Veliça P, Rundqvist H, Fitzpatrick S, et al. An HIF-1α/VEGF-A Axis in Cytotoxic T Cells Regulates Tumor Progression. Cancer Cell. 2017;32:669-683.e5 pubmed publisher
Gordón Alonso M, Hirsch T, Wildmann C, van der Bruggen P. Galectin-3 captures interferon-gamma in the tumor matrix reducing chemokine gradient production and T-cell tumor infiltration. Nat Commun. 2017;8:793 pubmed publisher
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Carpentier A, Geinguenaud F, Tran T, Sejalon F, Martin A, Motte L, et al. Synthetic melanin bound to subunit vaccine antigens significantly enhances CD8+ T-cell responses. PLoS ONE. 2017;12:e0181403 pubmed publisher
Griffin B, Muthumani K, Warner B, Majer A, Hagan M, Audet J, et al. DNA vaccination protects mice against Zika virus-induced damage to the testes. Nat Commun. 2017;8:15743 pubmed publisher
Donnarumma T, Young G, Merkenschlager J, Eksmond U, Bongard N, Nutt S, et al. Opposing Development of Cytotoxic and Follicular Helper CD4 T Cells Controlled by the TCF-1-Bcl6 Nexus. Cell Rep. 2016;17:1571-1583 pubmed publisher
Mackroth M, Abel A, Steeg C, Schulze zur Wiesch J, Jacobs T. Acute Malaria Induces PD1+CTLA4+ Effector T Cells with Cell-Extrinsic Suppressor Function. PLoS Pathog. 2016;12:e1005909 pubmed publisher
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Coomes S, Kannan Y, Pelly V, Entwistle L, Guidi R, Perez Lloret J, et al. CD4+ Th2 cells are directly regulated by IL-10 during allergic airway inflammation. Mucosal Immunol. 2017;10:150-161 pubmed publisher
Pachnio A, Zuo J, Ryan G, Begum J, Moss P. The Cellular Localization of Human Cytomegalovirus Glycoprotein Expression Greatly Influences the Frequency and Functional Phenotype of Specific CD4+ T Cell Responses. J Immunol. 2015;195:3803-15 pubmed publisher
product information
Antigen :
Granzyme B
Apps. Abbrev. :
ICFC
Cat # :
515405
Clone :
GB11
Item :
Alexa Fluor® 647 anti-human/mouse Granzyme B
Isotype :
Mouse IgG1, κ
Other Names :
Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
Size :
25 tests
Price (USD) :
171 USD
Reactivity :
Human, Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A647
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA