LEAF™ Purified anti-human IFN-γ | BioLegend 507513 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human IFN-γ

catalog :

507513

quantity :

500 μg

price :

320 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MD-1

reactivity :

human, Rhesus monkey

application :

western blot, ELISA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; fig s4	Chang D, Moniz R, Xu Z, Sun J, Signoretti S, Zhu Q, et al. Human anti-CAIX antibodies mediate immune cell inhibition of renal cell carcinoma in vitro and in a humanized mouse model in vivo. Mol Cancer. 2015;14:119 pubmed publisher

product information

Antigen :

IFN-g

Apps. Abbrev. :

ELISA Capture, Intracellular Staining for Flow Cytometry, IHC-F, Western Blotting

Cat # :

507513

Clone :

MD-1

Item :

LEAF™ Purified anti-human IFN-γ

Isotype :

Mouse IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma

Size :

500 μg

Price (USD) :

320 USD

Reactivity :

Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

ELISA 1,2,5 and ELISPOT 3,4 Capture: The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. The LEAF™ Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 µg/ml is recommended. Neutralization 1 : The LEAF™ Purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 507513). Additional reported applications (for the relevant formats) include: Western blotting 1,2 , intracellular staining for flow cytometry 6 , and immunohistochemical staining 7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections. Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.