Purified anti-human IFN-γ | BioLegend 507501 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-human IFN-γ

catalog :

507501

quantity :

50 μg

price :

106 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MD-1

reactivity :

human

application :

western blot, ELISA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

BioLegend product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; fig s4	Chang D, Moniz R, Xu Z, Sun J, Signoretti S, Zhu Q, et al. Human anti-CAIX antibodies mediate immune cell inhibition of renal cell carcinoma in vitro and in a humanized mouse model in vivo. Mol Cancer. 2015;14:119 pubmed publisher
	Aguilar Briseño J, Upasani V, Ellen B, Moser J, Pauzuolis M, Ruiz Silva M, et al. TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis. Nat Commun. 2020;11:3177 pubmed publisher

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

ELISA Capture, Intracellular Staining for Flow Cytometry, IHC-F, Western Blotting

Cat # :

507501

Clone :

MD-1

Item :

Purified anti-human IFN-γ

Isotype :

Mouse IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma

Size :

50 μg

Price (USD) :

106 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PURE

Application Notes :

ELISA 1,2,5 and ELISPOT 3,4 Capture: The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN- (Cat. No. 570209) as the standard. The LEAF Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 ug/ml is recommended. Neutralization 1 : The LEAF Purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of human IFN- bioactivity (Cat. No. 507513). Additional reported applications (for the relevant formats) include: Western blotting 1,2 , intracellular staining for flow cytometry 6 , and immunohistochemical staining 7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections. Note: For testing human IFN- in serum or plasma, BioLegend s ELISA Max Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

more info or order :

BioLegend product webpage