PE/Dazzle™ 594 anti-human IL-10 | BioLegend 506811 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Dazzle™ 594 anti-human IL-10

catalog :

506811

quantity :

25 tests

price :

141 USD

clonality :

monoclonal

host :

rat

conjugate :

clone name :

JES3-19F1

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3d	Molnar C, Scherer A, Baraliakos X, de Hooge M, Micheroli R, Exer P, et al. TNF blockers inhibit spinal radiographic progression in ankylosing spondylitis by reducing disease activity: results from the Swiss Clinical Quality Management cohort. Ann Rheum Dis. 2018;77:63-69 pubmed publisher
	Zhang B, Sun J, Yuan Y, Ji D, Sun Y, Liu Y, et al. Proximity-enabled covalent binding of IL-2 to IL-2Rα selectively activates regulatory T cells and suppresses autoimmunity. Signal Transduct Target Ther. 2023;8:28 pubmed publisher
	Yu E, Wang E, Garrigan E, Sutherland A, Khalil N, Kearns K, et al. Ex vivo assays show human gamma-delta T cells specific for common allergens are Th1-polarized in allergic donors. Cell Rep Methods. 2022;2:100350 pubmed publisher
	Riese P, Trittel S, Akmatov M, May M, Prokein J, Illig T, et al. Distinct immunological and molecular signatures underpinning influenza vaccine responsiveness in the elderly. Nat Commun. 2022;13:6894 pubmed publisher
	Yang R, Mele F, Worley L, Langlais D, Rosain J, Benhsaien I, et al. Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. Cell. 2020;183:1826-1847.e31 pubmed publisher
	Rydyznski Moderbacher C, Ramirez S, Dan J, Grifoni A, Hastie K, Weiskopf D, et al. Antigen-Specific Adaptive Immunity to SARS-CoV-2 in Acute COVID-19 and Associations with Age and Disease Severity. Cell. 2020;183:996-1012.e19 pubmed publisher
	Ping Y, Li F, Nan S, Zhang D, Shi X, Shan J, et al. Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFv. Front Cell Dev Biol. 2020;8:803 pubmed publisher

product information

Antigen :

IL-10

Apps. Abbrev. :

ICFC

Cat # :

506811

Clone :

JES3-19F1

Item :

PE/Dazzle™ 594 anti-human IL-10

Isotype :

Rat IgG2a, κ

Other Names :

Interleukin 10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)

Size :

25 tests

Price (USD) :

141 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PE_DZL594

Immunogen :

COS-expressed recombinant human IL-10

Application Notes :

ELISA or ELISPOT Capture^1-4: The Purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. Nos. 501502 & 501503) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 µg/ml is recommended.Flow Cytometry: The fluorochrome-labeled JES3-19F1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization^1,2: The Ultra-LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 506814 & 506815).Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining^5,6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430601 to 430606) are specially developed and recommended.;

more info or order :

BioLegend product webpage