LEAF™ Purified anti-human IL-10 | BioLegend 506810 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human IL-10

catalog :

506810

quantity :

500 μg

price :

270 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

JES3-19F1

reactivity :

black flying fox, human

application :

western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

citations: 6

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3d	Molnar C, Scherer A, Baraliakos X, de Hooge M, Micheroli R, Exer P, et al. TNF blockers inhibit spinal radiographic progression in ankylosing spondylitis by reducing disease activity: results from the Swiss Clinical Quality Management cohort. Ann Rheum Dis. 2018;77:63-69 pubmed publisher
flow cytometry; black flying fox; loading ...; fig 6b	Martínez Gómez J, Periasamy P, Dutertre C, Irving A, Ng J, Crameri G, et al. Phenotypic and functional characterization of the major lymphocyte populations in the fruit-eating bat Pteropus alecto. Sci Rep. 2016;6:37796 pubmed publisher
blocking or activating experiments; human	Jin J, Zhang W, Wong K, Kwak M, van Driel I, Yu Q. Inhibition of breast cancer resistance protein (ABCG2) in human myeloid dendritic cells induces potent tolerogenic functions during LPS stimulation. PLoS ONE. 2014;9:e104753 pubmed publisher
	Chan Y, Zuo J, Inman C, Croft W, Begum J, Croudace J, et al. NK cells produce high levels of IL-10 early after allogeneic stem cell transplantation and suppress development of acute GVHD. Eur J Immunol. 2018;48:316-329 pubmed publisher
	Islas Vazquez L, Prado Garcia H, Aguilar Cazares D, Meneses Flores M, Galicia Velasco M, Romero Garcia S, et al. LAP TGF-Beta Subset of CD4(+)CD25(+)CD127(-) Treg Cells is Increased and Overexpresses LAP TGF-Beta in Lung Adenocarcinoma Patients. Biomed Res Int. 2015;2015:430943 pubmed publisher
	Cates A, Holden V, Myers E, Smith C, Kaplan M, Kahlenberg J. Interleukin 10 hampers endothelial cell differentiation and enhances the effects of interferon Î± on lupus endothelial cell progenitors. Rheumatology (Oxford). 2015;54:1114-23 pubmed publisher

product information

Antigen :

IL-10

Apps. Abbrev. :

ELISA Capture, IHC-F, ICC, Western Blotting

Cat # :

506810

Clone :

JES3-19F1

Item :

LEAF™ Purified anti-human IL-10

Isotype :

Rat IgG2a, κ

Other Names :

Interleukin 10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)

Size :

500 μg

Price (USD) :

270 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

COS-expressed recombinant human IL-10

Application Notes :

ELISA or ELISPOT Capture 1-4 : The Purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. Nos. 501502 501503) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 µg/ml is recommended. Flow Cytometry: The fluorochrome-labeled JES3-19F1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization 1,2 : The Ultra-LEAF™ Purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 506814 506815). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining 5,6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430601 to 430606) are specially developed and recommended.