PE anti-human IL-10 | BioLegend 506804 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE anti-human IL-10

catalog :

506804

quantity :

100 tests

price :

214 USD

clonality :

monoclonal

host :

rat

conjugate :

clone name :

JES3-19F1

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3d	Molnar C, Scherer A, Baraliakos X, de Hooge M, Micheroli R, Exer P, et al. TNF blockers inhibit spinal radiographic progression in ankylosing spondylitis by reducing disease activity: results from the Swiss Clinical Quality Management cohort. Ann Rheum Dis. 2018;77:63-69 pubmed publisher
flow cytometry; human	Misra R, Shah S, Fowell D, Wang H, Scheible K, Misra S, et al. Preterm cord blood CD4âº T cells exhibit increased IL-6 production in chorioamnionitis and decreased CD4âº T cells in bronchopulmonary dysplasia. Hum Immunol. 2015;76:329-338 pubmed publisher
	Piao H, Fu L, Wang Y, Liu Y, Wang Y, Meng X, et al. A positive feedback loop between gastric cancer cells and tumor-associated macrophage induces malignancy progression. J Exp Clin Cancer Res. 2022;41:174 pubmed publisher
	de Jonge K, Tillé L, Lourenço J, Maby El Hajjami H, Nassiri S, Racle J, et al. Inflammatory B cells correlate with failure to checkpoint blockade in melanoma patients. Oncoimmunology. 2021;10:1873585 pubmed publisher
	Cerny V, Petrásková P, Novotná O, Boráková K, Prokešová L, Kolarova L, et al. Value of cord blood Treg population properties and function-associated characteristics for predicting allergy development in childhood. Cent Eur J Immunol. 2020;45:393-402 pubmed publisher
	Benner M, Feyaerts D, García C, Inci N, López S, Fasse E, et al. Clusters of Tolerogenic B Cells Feature in the Dynamic Immunological Landscape of the Pregnant Uterus. Cell Rep. 2020;32:108204 pubmed publisher
	Zarobkiewicz M, Kowalska W, Chocholska S, Tomczak W, Szymańska A, Morawska I, et al. High M-MDSC Percentage as a Negative Prognostic Factor in Chronic Lymphocytic Leukaemia. Cancers (Basel). 2020;12: pubmed publisher
	Aliu B, Demeestere D, Seydoux E, Boucraut J, Delmont E, Brodovitch A, et al. Selective inhibition of anti-MAG IgM autoantibody binding to myelin by an antigen-specific glycopolymer. J Neurochem. 2020;154:486-501 pubmed publisher
	de Jonge K, Ebering A, Nassiri S, Maby El Hajjami H, Ouertatani Sakouhi H, Baumgaertner P, et al. Circulating CD56bright NK cells inversely correlate with survival of melanoma patients. Sci Rep. 2019;9:4487 pubmed publisher
	Baguma R, Penn Nicholson A, Smit E, Erasmus M, Day J, Makhethe L, et al. Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population. PLoS ONE. 2017;12:e0184563 pubmed publisher
	Zischke J, Mamareli P, Pokoyski C, Gabaev I, Buyny S, Jacobs R, et al. The human cytomegalovirus glycoprotein pUL11 acts via CD45 to induce T cell IL-10 secretion. PLoS Pathog. 2017;13:e1006454 pubmed publisher
	Liu Y, Bezverbnaya K, Zhao T, Parsons M, Shi M, Treanor B, et al. Involvement of the HCK and FGR src-family kinases in FCRL4-mediated immune regulation. J Immunol. 2015;194:5851-60 pubmed publisher
	Boyle M, Jagannathan P, Bowen K, McIntyre T, Vance H, Farrington L, et al. Effector Phenotype of Plasmodium falciparum-Specific CD4+ T Cells Is Influenced by Both Age and Transmission Intensity in Naturally Exposed Populations. J Infect Dis. 2015;212:416-25 pubmed publisher
	Jin J, Zhang W, Wong K, Kwak M, van Driel I, Yu Q. Inhibition of breast cancer resistance protein (ABCG2) in human myeloid dendritic cells induces potent tolerogenic functions during LPS stimulation. PLoS ONE. 2014;9:e104753 pubmed publisher
	Shey M, Nemes E, Whatney W, de Kock M, Africa H, Barnard C, et al. Maturation of innate responses to mycobacteria over the first nine months of life. J Immunol. 2014;192:4833-43 pubmed publisher

product information

Antigen :

IL-10

Apps. Abbrev. :

ICFC

Cat # :

506804

Clone :

JES3-19F1

Item :

PE anti-human IL-10

Isotype :

Rat IgG2a, κ

Other Names :

Interleukin 10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)

Size :

100 tests

Price (USD) :

214 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

Immunogen :

COS-expressed recombinant human IL-10

Application Notes :

ELISA or ELISPOT Capture^1-4: The Purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. Nos. 501502 & 501503) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 µg/ml is recommended.Flow Cytometry: The fluorochrome-labeled JES3-19F1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization^1,2: The Ultra-LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 506814 & 506815).Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining^5,6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430601 to 430606) are specially developed and recommended.;

more info or order :

BioLegend product webpage