Purified anti-human IL-10 | BioLegend 506802 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-human IL-10

catalog :

506802

quantity :

500 μg

price :

238 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

JES3-19F1

reactivity :

black flying fox, human

application :

western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3d	Molnar C, Scherer A, Baraliakos X, de Hooge M, Micheroli R, Exer P, et al. TNF blockers inhibit spinal radiographic progression in ankylosing spondylitis by reducing disease activity: results from the Swiss Clinical Quality Management cohort. Ann Rheum Dis. 2018;77:63-69 pubmed publisher
flow cytometry; black flying fox; loading ...; fig 6b	Martínez Gómez J, Periasamy P, Dutertre C, Irving A, Ng J, Crameri G, et al. Phenotypic and functional characterization of the major lymphocyte populations in the fruit-eating bat Pteropus alecto. Sci Rep. 2016;6:37796 pubmed publisher
blocking or activating experiments; human	Jin J, Zhang W, Wong K, Kwak M, van Driel I, Yu Q. Inhibition of breast cancer resistance protein (ABCG2) in human myeloid dendritic cells induces potent tolerogenic functions during LPS stimulation. PLoS ONE. 2014;9:e104753 pubmed publisher
	He Y, Ge C, Moreno Gir xf3 x, Xu B, Beusch C, Sandor K, et al. A subset of antibodies targeting citrullinated proteins confers protection from rheumatoid arthritis. Nat Commun. 2023;14:691 pubmed publisher
	Gilboa T, Maley A, Ogata A, Wu C, Walt D. Sequential Protein Capture in Multiplex Single Molecule Arrays: A Strategy for Eliminating Assay Cross-Reactivity. Adv Healthc Mater. 2021;10:e2001111 pubmed publisher
	Yan J, Pandey S, Barnes B, Turner J, Abraham C. T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation. Cell Rep. 2020;31:107820 pubmed publisher
	Haug V, Kollar B, Tasigiorgos S, Endo Y, Kauke M, Safi A, et al. Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours. Transplantation. 2020;104:e260-e270 pubmed publisher
	Stras S, Werner L, Toothaker J, Olaloye O, Oldham A, McCourt C, et al. Maturation of the Human Intestinal Immune System Occurs Early in Fetal Development. Dev Cell. 2019;51:357-373.e5 pubmed publisher
	Ni G, Chen S, Yuan J, Cavezza S, Wei M, Li H, et al. Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells. PLoS ONE. 2019;14:e0213813 pubmed publisher
	Pararasa C, Zhang N, Tull T, Chong M, Siu J, Guesdon W, et al. Reduced CD27-IgD- B Cells in Blood and Raised CD27-IgD- B Cells in Gut-Associated Lymphoid Tissue in Inflammatory Bowel Disease. Front Immunol. 2019;10:361 pubmed publisher
	Wang W, Marinis J, Beal A, Savadkar S, Wu Y, Khan M, et al. RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer. Cancer Cell. 2018;34:757-774.e7 pubmed publisher
	Lane D, Matte I, Garde Granger P, Bessette P, Piche A. Ascites IL-10 Promotes Ovarian Cancer Cell Migration. Cancer Microenviron. 2018;11:115-124 pubmed publisher
	Chan Y, Zuo J, Inman C, Croft W, Begum J, Croudace J, et al. NK cells produce high levels of IL-10 early after allogeneic stem cell transplantation and suppress development of acute GVHD. Eur J Immunol. 2018;48:316-329 pubmed publisher
	Islas Vazquez L, Prado Garcia H, Aguilar Cazares D, Meneses Flores M, Galicia Velasco M, Romero Garcia S, et al. LAP TGF-Beta Subset of CD4(+)CD25(+)CD127(-) Treg Cells is Increased and Overexpresses LAP TGF-Beta in Lung Adenocarcinoma Patients. Biomed Res Int. 2015;2015:430943 pubmed publisher

product information

Antigen :

IL-10

Apps. Abbrev. :

ELISA Capture, IHC-F, ICC, WB

Cat # :

506802

Clone :

JES3-19F1

Item :

Purified anti-human IL-10

Isotype :

Rat IgG2a, κ

Other Names :

Interleukin 10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)

Size :

500 μg

Price (USD) :

238 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PURE

Immunogen :

COS-expressed recombinant human IL-10

Application Notes :

ELISA or ELISPOT Capture^1-4: The Purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. Nos. 501502 & 501503) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 µg/ml is recommended.Flow Cytometry: The fluorochrome-labeled JES3-19F1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization^1,2: The Ultra-LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 506814 & 506815).Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining^5,6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430601 to 430606) are specially developed and recommended.;

more info or order :

BioLegend product webpage