GoInVivo™ Purified anti-mouse TNF-α | BioLegend 506353 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

GoInVivo™ Purified anti-mouse TNF-α

catalog :

506353

quantity :

50 mg

price :

3575 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MP6-XT22

reactivity :

mouse

application :

western blot, ELISA, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section

more info or order :

BioLegend product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:200; loading ...; fig 3j	Amoozgar Z, Kloepper J, Ren J, Tay R, Kazer S, Kiner E, et al. Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12:2582 pubmed publisher
	Janova H, Zhao F, Desai P, Mack M, Thackray L, Stappenbeck T, et al. West Nile virus triggers intestinal dysmotility via T cell-mediated enteric nervous system injury. J Clin Invest. 2024;134: pubmed publisher
	Reed J, Preston Hurlburt P, Philbrick W, Betancur G, Korah M, LUCAS C, et al. The receptor for advanced glycation endproducts (RAGE) modulates T cell signaling. PLoS ONE. 2020;15:e0236921 pubmed publisher
	Katsumata K, Ishihara J, Fukunaga K, Ishihara A, Yuba E, Budina E, et al. Conferring extracellular matrix affinity enhances local therapeutic efficacy of anti-TNF-α antibody in a murine model of rheumatoid arthritis. Arthritis Res Ther. 2019;21:298 pubmed publisher

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

ELISA, ICFC, CyTOF®, ICC, IHC-F, WB, Neut

Cat # :

506353

Clone :

MP6-XT22

Item :

GoInVivo™ Purified anti-mouse TNF-α

Isotype :

Rat IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha

Size :

50 mg

Price (USD) :

3575 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

GOINVIVO

Immunogen :

E. coli -expressed, recombinant mouse TNF-α

Application Notes :

ELISA or ELISPOT Detection: The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 & 510804) as the capture antibody.ELISA Capture: The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. No. 575209) as the standard.Flow Cytometry^6,11,12:The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Neutralization^1,5,10,16,17:The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF-α. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse TNF-α bioactivity in vivo and in vitro(Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^7-9 in vivo detection^5, immunofluorescence, and immunocytochemistry.Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901) are specially developed and recommended.;

more info or order :

BioLegend product webpage