APC/Cyanine7 anti-mouse TNF-α | BioLegend 506343 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC/Cyanine7 anti-mouse TNF-α

catalog :

506343

quantity :

25 μg

price :

141 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MP6-XT22

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 5

Reference
Shi Z, Qiu Y, Zhao Z, Wen D, Chen H, Du Q, et al. CD8+ T cell subpopulations and pro-inflammatory cytokines in neuromyelitis optica spectrum disorder. Ann Clin Transl Neurol. 2021;8:43-53 pubmed publisher
Angiari S, Runtsch M, Sutton C, Palsson McDermott E, Kelly B, Rana N, et al. Pharmacological Activation of Pyruvate Kinase M2 Inhibits CD4+ T Cell Pathogenicity and Suppresses Autoimmunity. Cell Metab. 2020;31:391-405.e8 pubmed publisher
Levesque S, Le Naour J, Pietrocola F, Paillet J, Kremer M, Castoldi F, et al. A synergistic triad of chemotherapy, immune checkpoint inhibitors, and caloric restriction mimetics eradicates tumors in mice. Oncoimmunology. 2019;8:e1657375 pubmed publisher
Chen S, Chen L, Lin D, Chen S, Tsao Y, Guo H, et al. NLRP12 Regulates Anti-viral RIG-I Activation via Interaction with TRIM25. Cell Host Microbe. 2019;25:602-616.e7 pubmed publisher
Djokić V, Primus S, Akoolo L, Chakraborti M, Parveen N. Age-Related Differential Stimulation of Immune Response by Babesia microti and Borrelia burgdorferi During Acute Phase of Infection Affects Disease Severity. Front Immunol. 2018;9:2891 pubmed publisher

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

506343

Clone :

MP6-XT22

Item :

APC/Cyanine7 anti-mouse TNF-α

Isotype :

Rat IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha

Size :

25 μg

Price (USD) :

141 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

APC_CYANINE7

Immunogen :

E. coli -expressed, recombinant mouse TNF-α

Application Notes :

ELISA or ELISPOT Detection : The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 510804) as the capture antibody. ELISA Capture : The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF- (Cat. No. 575209) as the standard. Flow Cytometry 6,11,12 :The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. Neutralization 1,5,10,16,17 :The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse TNF- bioactivity in vivo and in vitro (Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include : Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 7-9 in vivo detection 5 , immunofluorescence, and immunocytochemistry. Note : For testing mouse TNF- in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430901) are specially developed and recommended.

more info or order :

BioLegend product webpage