PE/Cyanine7 anti-mouse TNF-α | BioLegend 506324 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine7 anti-mouse TNF-α

catalog :

506324

quantity :

100 μg

price :

303 USD

clonality :

monoclonal

host :

rat

conjugate :

PE/Cy7

clone name :

MP6-XT22

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 33

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s1d	Li Y, Tinoco R, Elmén L, Segota I, Xian Y, Fujita Y, et al. Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5-/- mice. Nat Commun. 2019;10:1492 pubmed publisher
flow cytometry; mouse; loading ...; fig 6b	McLaren J, Clement M, Marsden M, Miners K, Llewellyn Lacey S, Grant E, et al. IL-33 Augments Virus-Specific Memory T Cell Inflation and Potentiates the Efficacy of an Attenuated Cytomegalovirus-Based Vaccine. J Immunol. 2019;202:943-955 pubmed publisher
	Song X, Li N, Liu Y, Wang Z, Wang T, Tan S, et al. CD169-positive macrophages enhance abscopal effect of radiofrequency ablation therapy in liver cancer. Transl Oncol. 2022;15:101306 pubmed publisher
	Cai B, Liu Y, Chong Y, Zhang H, Matsunaga A, Fang X, et al. IRAK1-regulated IFN-γ signaling induces MDSC to facilitate immune evasion in FGFR1-driven hematological malignancies. Mol Cancer. 2021;20:165 pubmed publisher
	Klarquist J, Cross E, Thompson S, Willett B, Aldridge D, Caffrey Carr A, et al. B cells promote CD8 T cell primary and memory responses to subunit vaccines. Cell Rep. 2021;36:109591 pubmed publisher
	Derada Troletti C, Enzmann G, Chiurchiù V, Kamermans A, Tietz S, Norris P, et al. Pro-resolving lipid mediator lipoxin A4 attenuates neuro-inflammation by modulating T cell responses and modifies the spinal cord lipidome. Cell Rep. 2021;35:109201 pubmed publisher
	Shemer A, Scheyltjens I, Frumer G, Kim J, Grozovski J, Ayanaw S, et al. Interleukin-10 Prevents Pathological Microglia Hyperactivation following Peripheral Endotoxin Challenge. Immunity. 2020;53:1033-1049.e7 pubmed publisher
	Acharya N, Madi A, Zhang H, Klapholz M, Escobar G, Dulberg S, et al. Endogenous Glucocorticoid Signaling Regulates CD8+ T Cell Differentiation and Development of Dysfunction in the Tumor Microenvironment. Immunity. 2020;53:658-671.e6 pubmed publisher
	Dai L, Zheng T, Xu K, Han Y, Xu L, Huang E, et al. A Universal Design of Betacoronavirus Vaccines against COVID-19, MERS, and SARS. Cell. 2020;182:722-733.e11 pubmed publisher
	Chauhan A, Sheriff L, Hussain M, Webb G, Patten D, Shepherd E, et al. The platelet receptor CLEC-2 blocks neutrophil mediated hepatic recovery in acetaminophen induced acute liver failure. Nat Commun. 2020;11:1939 pubmed publisher
	Zhang W, Xu L, Park H, Hwang J, Kwak M, Lee P, et al. Escherichia coli adhesion portion FimH functions as an adjuvant for cancer immunotherapy. Nat Commun. 2020;11:1187 pubmed publisher
	Kumar R, Bunn P, Singh S, Ng S, Montes De Oca M, de Labastida Rivera F, et al. Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis. Cell Rep. 2020;30:2512-2525.e9 pubmed publisher
	Li Y, Elmén L, Segota I, Xian Y, Tinoco R, Feng Y, et al. Prebiotic-Induced Anti-tumor Immunity Attenuates Tumor Growth. Cell Rep. 2020;30:1753-1766.e6 pubmed publisher
	Morris A, Farley C, Pinelli D, Adams L, Cragg M, Boss J, et al. Signaling through the Inhibitory Fc Receptor FcγRIIB Induces CD8+ T Cell Apoptosis to Limit T Cell Immunity. Immunity. 2020;52:136-150.e6 pubmed publisher
	Eckert E, Nace R, Tonne J, Evgin L, Vile R, Russell S. Generation of a Tumor-Specific Chemokine Gradient Using Oncolytic Vesicular Stomatitis Virus Encoding CXCL9. Mol Ther Oncolytics. 2020;16:63-74 pubmed publisher
	Sledzińska A, Vila de Mucha M, Bergerhoff K, Hotblack A, Demane D, Ghorani E, et al. Regulatory T Cells Restrain Interleukin-2- and Blimp-1-Dependent Acquisition of Cytotoxic Function by CD4+ T Cells. Immunity. 2020;52:151-166.e6 pubmed publisher
	Kim C, Jadhav R, Gustafson C, Smithey M, Hirsch A, Uhrlaub J, et al. Defects in Antiviral T Cell Responses Inflicted by Aging-Associated miR-181a Deficiency. Cell Rep. 2019;29:2202-2216.e5 pubmed publisher
	Kurtulus S, Madi A, Escobar G, Klapholz M, Nyman J, Christian E, et al. Checkpoint Blockade Immunotherapy Induces Dynamic Changes in PD-1-CD8+ Tumor-Infiltrating T Cells. Immunity. 2019;50:181-194.e6 pubmed publisher
	Fujita Y, Khateb A, Li Y, Tinoco R, Zhang T, Bar Yoseph H, et al. Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis. Cell Rep. 2018;24:3296-3311.e6 pubmed publisher
	Syed I, Lee J, Moraes Vieira P, Donaldson C, Sontheimer A, Aryal P, et al. Palmitic Acid Hydroxystearic Acids Activate GPR40, Which Is Involved in Their Beneficial Effects on Glucose Homeostasis. Cell Metab. 2018;27:419-427.e4 pubmed publisher
	Sidhom J, Bessell C, Havel J, Kosmides A, Chan T, Schneck J. ImmunoMap: A Bioinformatics Tool for T-cell Repertoire Analysis. Cancer Immunol Res. 2018;6:151-162 pubmed publisher
	Cao W, Kayama H, Chen M, Delmas A, Sun A, Kim S, et al. The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids. Immunity. 2017;47:1182-1196.e10 pubmed publisher
	Zaitseva M, Thomas A, Meseda C, Cheung C, Diaz C, Xiang Y, et al. Development of an animal model of progressive vaccinia in nu/nu mice and the use of bioluminescence imaging for assessment of the efficacy of monoclonal antibodies against vaccinial B5 and L1 proteins. Antiviral Res. 2017;144:8-20 pubmed publisher
	Jiang J, Liu C, Liu M, Shen Y, Hu X, Wang Q, et al. OX40 signaling is involved in the autoactivation of CD4+CD28- T cells and contributes to the pathogenesis of autoimmune arthritis. Arthritis Res Ther. 2017;19:67 pubmed publisher
	Vicetti Miguel R, Quispe Calla N, Pavelko S, Cherpes T. Intravaginal Chlamydia trachomatis Challenge Infection Elicits TH1 and TH17 Immune Responses in Mice That Promote Pathogen Clearance and Genital Tract Damage. PLoS ONE. 2016;11:e0162445 pubmed publisher
	Latasa C, Echeverz M, García B, Gil C, García Ona E, Burgui S, et al. Evaluation of a Salmonella Strain Lacking the Secondary Messenger C-di-GMP and RpoS as a Live Oral Vaccine. PLoS ONE. 2016;11:e0161216 pubmed publisher
	Lin D, Lei L, Liu Y, Zhang Y, Hu B, Bao G, et al. Membrane IL1? Inhibits the Development of Hepatocellular Carcinoma via Promoting T- and NK-cell Activation. Cancer Res. 2016;76:3179-88 pubmed publisher
	Uddback I, Pedersen L, Pedersen S, Steffensen M, Holst P, Thomsen A, et al. Combined local and systemic immunization is essential for durable T-cell mediated heterosubtypic immunity against influenza A virus. Sci Rep. 2016;6:20137 pubmed publisher
	Correia A, Ferreirinha P, Botelho S, Belinha A, Leitao C, Caramalho I, et al. Predominant role of interferon-γ in the host protective effect of CD8(+) T cells against Neospora caninum infection. Sci Rep. 2015;5:14913 pubmed publisher
	Kersh A, Edwards L, Evavold B. Progression of relapsing-remitting demyelinating disease does not require increased TCR affinity or epitope spread. J Immunol. 2014;193:4429-38 pubmed publisher
	Carlson M, West M, COGHILL J, Panoskaltsis Mortari A, Blazar B, Serody J. In vitro-differentiated TH17 cells mediate lethal acute graft-versus-host disease with severe cutaneous and pulmonary pathologic manifestations. Blood. 2009;113:1365-74 pubmed publisher
	Wu S, Ren J. Benfotiamine alleviates diabetes-induced cerebral oxidative damage independent of advanced glycation end-product, tissue factor and TNF-alpha. Neurosci Lett. 2006;394:158-62 pubmed
	Patole P, Zecher D, Pawar R, Grone H, Schlondorff D, Anders H. G-rich DNA suppresses systemic lupus. J Am Soc Nephrol. 2005;16:3273-80 pubmed

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

506324

Clone :

MP6-XT22

Item :

PE/Cyanine7 anti-mouse TNF-α

Isotype :

Rat IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha

Size :

100 μg

Price (USD) :

303 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PE_CYANINE7

Immunogen :

E. coli -expressed, recombinant mouse TNF-α

Application Notes :

ELISA or ELISPOT Detection : The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 510804) as the capture antibody. ELISA Capture : The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF- (Cat. No. 575209) as the standard. Flow Cytometry 6,11,12 :The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. Neutralization 1,5,10,16,17 :The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse TNF- bioactivity in vivo and in vitro (Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include : Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 7-9 in vivo detection 5 , immunofluorescence, and immunocytochemistry. Note : For testing mouse TNF- in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430901) are specially developed and recommended.

more info or order :

BioLegend product webpage