Pacific Blue™ anti-mouse TNF-α | BioLegend 506318 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Pacific Blue™ anti-mouse TNF-α

catalog :

506318

quantity :

100 μg

price :

265 USD

clonality :

monoclonal

host :

rat

conjugate :

Pacific Blue

clone name :

MP6-XT22

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 4

Reference
Baban B, Braun M, Khodadadi H, Ward A, Alverson K, Malik A, et al. AMPK induces regulatory innate lymphoid cells after traumatic brain injury. JCI Insight. 2021;6: pubmed publisher
Fox A, Dutt T, Karger B, Obregón Henao A, Anderson G, Henao Tamayo M. Acquisition of High-Quality Spectral Flow Cytometry Data. Curr Protoc Cytom. 2020;93:e74 pubmed publisher
Gomez Cadena A, Urueña C, Prieto K, Martinez Usatorre A, Donda A, Barreto A, et al. Immune-system-dependent anti-tumor activity of a plant-derived polyphenol rich fraction in a melanoma mouse model. Cell Death Dis. 2016;7:e2243 pubmed publisher
Blank J, Behrends J, Jacobs T, Schneider B. Mycobacterium tuberculosis Coinfection Has No Impact on Plasmodium berghei ANKA-Induced Experimental Cerebral Malaria in C57BL/6 Mice. Infect Immun. 2016;84:502-10 pubmed publisher

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

506318

Clone :

MP6-XT22

Item :

Pacific Blue™ anti-mouse TNF-α

Isotype :

Rat IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha

Size :

100 μg

Price (USD) :

265 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

Immunogen :

E. coli -expressed, recombinant mouse TNF-α

Application Notes :

ELISA or ELISPOT Detection : The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 510804) as the capture antibody. ELISA Capture : The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF- (Cat. No. 575209) as the standard. Flow Cytometry 6,11,12 :The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. Neutralization 1,5,10,16,17 :The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse TNF- bioactivity in vivo and in vitro (Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include : Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 7-9 in vivo detection 5 , immunofluorescence, and immunocytochemistry. Note : For testing mouse TNF- in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430901) are specially developed and recommended.

more info or order :

BioLegend product webpage