Biotin anti-mouse TNF-α | BioLegend 506312 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Biotin anti-mouse TNF-α

catalog :

506312

quantity :

500 μg

price :

230 USD

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

MP6-XT22

reactivity :

mouse

application :

ELISA

more info or order :

BioLegend product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
ELISA; mouse; fig 5	Lee J, Park K, Han D, Bang N, Kim D, Na H, et al. PharmDB-K: Integrated Bio-Pharmacological Network Database for Traditional Korean Medicine. PLoS ONE. 2015;10:e0142624 pubmed publisher
	Condotta S, Khan S, Rai D, Griffith T, Badovinac V. Polymicrobial Sepsis Increases Susceptibility to Chronic Viral Infection and Exacerbates CD8+ T Cell Exhaustion. J Immunol. 2015;195:116-25 pubmed publisher
	Zuo D, Zhang L, Lu X, Liu Y, Chen Z. Protective role of mouse MBL-C on intestinal mucosa during Shigella flexneri invasion. Int Immunol. 2009;21:1125-34 pubmed publisher

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

ELISA Detection, ELISPOT Detection, Intracellular Staining for Flow Cytometry

Cat # :

506312

Clone :

MP6-XT22

Item :

Biotin anti-mouse TNF-α

Isotype :

Rat IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha

Size :

500 μg

Price (USD) :

230 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BIOTIN

Immunogen :

E. coli -expressed, recombinant mouse TNF-α

Application Notes :

ELISA or ELISPOT Detection: The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 510804) as the capture antibody. ELISA Capture : The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. No. 575809) as the standard. Flow Cytometry 6,11,12 : The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-α-producing cells within mixed cell populations. Neutralization 1,5,10,16,17 : The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF-α. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse TNF-α bioactivity in vivo and in vitro (Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 7-9 , in vivo detection 5 , immunofluorescence, and immunocytochemistry. Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901 to 430906) are specially developed and recommended.

more info or order :

BioLegend product webpage