product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse TNF-α
catalog :
506308
quantity :
100 μg
price :
264 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
MP6-XT22
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 52
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...
Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Blagih J, Zani F, Chakravarty P, Hennequart M, Pilley S, Hobor S, et al. Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses. Cell Rep. 2020;30:481-496.e6 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Misumi I, Starmer J, Uchimura T, Beck M, Magnuson T, Whitmire J. Obesity Expands a Distinct Population of T Cells in Adipose Tissue and Increases Vulnerability to Infection. Cell Rep. 2019;27:514-524.e5 pubmed publisher
  • flow cytometry; mouse; fig 1a
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c, 2g
Ding L, Kim H, Wang Q, Kearns M, Jiang T, Ohlson C, et al. PARP Inhibition Elicits STING-Dependent Antitumor Immunity in Brca1-Deficient Ovarian Cancer. Cell Rep. 2018;25:2972-2980.e5 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s7
Vuckovic S, Minnie S, Smith D, Gartlan K, Watkins T, Markey K, et al. Bone marrow transplantation generates T cell-dependent control of myeloma in mice. J Clin Invest. 2019;129:106-121 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e10a
Wan X, Zinselmeyer B, Zakharov P, Vomund A, Taniguchi R, Santambrogio L, et al. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Nature. 2018;560:107-111 pubmed publisher
  • flow cytometry; mouse; fig 6a
Clausen B, Degn M, Sivasaravanaparan M, Fogtmann T, Andersen M, Trojanowsky M, et al. Conditional ablation of myeloid TNF increases lesion volume after experimental stroke in mice, possibly via altered ERK1/2 signaling. Sci Rep. 2016;6:29291 pubmed publisher
  • flow cytometry; mouse; fig 3
Li J, Chassaing B, Tyagi A, Vaccaro C, Luo T, Adams J, et al. Sex steroid deficiency-associated bone loss is microbiota dependent and prevented by probiotics. J Clin Invest. 2016;126:2049-63 pubmed publisher
  • flow cytometry; mouse; 1:50; fig 3
Elahy M, Jackaman C, Mamo J, Lam V, Dhaliwal S, Giles C, et al. Blood-brain barrier dysfunction developed during normal aging is associated with inflammation and loss of tight junctions but not with leukocyte recruitment. Immun Ageing. 2015;12:2 pubmed publisher
  • flow cytometry; mouse
White C, Villarino N, Sloan S, Ganusov V, Schmidt N. Plasmodium suppresses expansion of T cell responses to heterologous infections. J Immunol. 2015;194:697-708 pubmed publisher
Zhang W, Wang Y, Li C, Xu Y, Wang X, Wu D, et al. Extracellular CIRP-Impaired Rab26 Restrains EPOR-Mediated Macrophage Polarization in Acute Lung Injury. Front Immunol. 2021;12:768435 pubmed publisher
Xu C, Sun S, Johnson T, Qi R, Zhang S, Zhang J, et al. The glutathione peroxidase Gpx4 prevents lipid peroxidation and ferroptosis to sustain Treg cell activation and suppression of antitumor immunity. Cell Rep. 2021;35:109235 pubmed publisher
Guo Y, Xie Y, Gao M, Zhao Y, Franco F, Wenes M, et al. Metabolic reprogramming of terminally exhausted CD8+ T cells by IL-10 enhances anti-tumor immunity. Nat Immunol. 2021;22:746-756 pubmed publisher
Dai X, Bu X, Gao Y, Guo J, Hu J, Jiang C, et al. Energy status dictates PD-L1 protein abundance and anti-tumor immunity to enable checkpoint blockade. Mol Cell. 2021;81:2317-2331.e6 pubmed publisher
Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
Davis F, Tsoi L, Melvin W, denDekker A, Wasikowski R, Joshi A, et al. Inhibition of macrophage histone demethylase JMJD3 protects against abdominal aortic aneurysms. J Exp Med. 2021;218: pubmed publisher
Li J, Hardy K, Olshansky M, Barugahare A, Gearing L, Prier J, et al. KDM6B-dependent chromatin remodeling underpins effective virus-specific CD8+ T cell differentiation. Cell Rep. 2021;34:108839 pubmed publisher
Jones N, Blagih J, Zani F, Rees A, Hill D, Jenkins B, et al. Fructose reprogrammes glutamine-dependent oxidative metabolism to support LPS-induced inflammation. Nat Commun. 2021;12:1209 pubmed publisher
Tanaka Y, Wada H, Goto R, Osada T, Yamamura K, Fukaya S, et al. TAS0314, a novel multi-epitope long peptide vaccine, showed synergistic antitumor immunity with PD-1/PD-L1 blockade in HLA-A*2402 mice. Sci Rep. 2020;10:17284 pubmed publisher
Sjaastad F, Kucaba T, Dileepan T, Swanson W, Dail C, Cabrera Perez J, et al. Polymicrobial Sepsis Impairs Antigen-Specific Memory CD4 T Cell-Mediated Immunity. Front Immunol. 2020;11:1786 pubmed publisher
Asadi Shahmirzadi A, Edgar D, Liao C, Hsu Y, Lucanic M, Asadi Shahmirzadi A, et al. Alpha-Ketoglutarate, an Endogenous Metabolite, Extends Lifespan and Compresses Morbidity in Aging Mice. Cell Metab. 2020;32:447-456.e6 pubmed publisher
Buqué A, Bloy N, Pérez Lanzón M, Iribarren K, Humeau J, Pol J, et al. Immunoprophylactic and immunotherapeutic control of hormone receptor-positive breast cancer. Nat Commun. 2020;11:3819 pubmed publisher
Kinder J, Turner L, Stelzer I, Miller Handley H, Burg A, Shao T, et al. CD8+ T Cell Functional Exhaustion Overrides Pregnancy-Induced Fetal Antigen Alloimmunization. Cell Rep. 2020;31:107784 pubmed publisher
Koh C, Kim I, Shin K, Jeon I, Song B, Lee J, et al. GITR Agonism Triggers Antitumor Immune Responses through IL21-Expressing Follicular Helper T Cells. Cancer Immunol Res. 2020;8:698-709 pubmed publisher
Yu X, Dai Y, Zhao Y, Qi S, Liu L, Lu L, et al. Melittin-lipid nanoparticles target to lymph nodes and elicit a systemic anti-tumor immune response. Nat Commun. 2020;11:1110 pubmed publisher
Koronyo Hamaoui M, Sheyn J, Hayden E, Li S, Fuchs D, Regis G, et al. Peripherally derived angiotensin converting enzyme-enhanced macrophages alleviate Alzheimer-related disease. Brain. 2020;143:336-358 pubmed publisher
Ge C, Monk I, Pizzolla A, Wang N, Bedford J, Stinear T, et al. Bystander Activation of Pulmonary Trm Cells Attenuates the Severity of Bacterial Pneumonia by Enhancing Neutrophil Recruitment. Cell Rep. 2019;29:4236-4244.e3 pubmed publisher
Lercher A, Bhattacharya A, Popa A, Caldera M, Schlapansky M, Baazim H, et al. Type I Interferon Signaling Disrupts the Hepatic Urea Cycle and Alters Systemic Metabolism to Suppress T Cell Function. Immunity. 2019;51:1074-1087.e9 pubmed publisher
Raju S, Verbaro D, Egawa T. PD-1 Signaling Promotes Control of Chronic Viral Infection by Restricting Type-I-Interferon-Mediated Tissue Damage. Cell Rep. 2019;29:2556-2564.e3 pubmed publisher
Davis F, Schaller M, denDekker A, Joshi A, Kimball A, Evanoff H, et al. Sepsis Induces Prolonged Epigenetic Modifications in Bone Marrow and Peripheral Macrophages Impairing Inflammation and Wound Healing. Arterioscler Thromb Vasc Biol. 2019;39:2353-2366 pubmed publisher
Di Mitri D, Mirenda M, Vasilevska J, Calcinotto A, Delaleu N, Revandkar A, et al. Re-education of Tumor-Associated Macrophages by CXCR2 Blockade Drives Senescence and Tumor Inhibition in Advanced Prostate Cancer. Cell Rep. 2019;28:2156-2168.e5 pubmed publisher
Kimball A, Davis F, denDekker A, Joshi A, Schaller M, Bermick J, et al. The Histone Methyltransferase Setdb2 Modulates Macrophage Phenotype and Uric Acid Production in Diabetic Wound Repair. Immunity. 2019;51:258-271.e5 pubmed publisher
Misumi I, Cook K, Mitchell J, Lund M, Vick S, Lee R, et al. Identification of a Locus in Mice that Regulates the Collateral Damage and Lethality of Virus Infection. Cell Rep. 2019;27:1387-1396.e5 pubmed publisher
Uchimura T, Oyama Y, Deng M, Guo H, Wilson J, Rampanelli E, et al. The Innate Immune Sensor NLRC3 Acts as a Rheostat that Fine-Tunes T Cell Responses in Infection and Autoimmunity. Immunity. 2018;49:1049-1061.e6 pubmed publisher
Nakata J, Nakajima H, Hayashibara H, Imafuku K, Morimoto S, Fujiki F, et al. Extremely strong infiltration of WT1-specific CTLs into mouse tumor by the combination vaccine with WT1-specific CTL and helper peptides. Oncotarget. 2018;9:36029-36038 pubmed publisher
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
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Kong X, Martinez Barricarte R, Kennedy J, Mele F, Lazarov T, Deenick E, et al. Disruption of an antimycobacterial circuit between dendritic and helper T cells in human SPPL2a deficiency. Nat Immunol. 2018;19:973-985 pubmed publisher
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product information
Antigen :
TNF-alpha
Apps. Abbrev. :
Intracellular Staining for Flow Cytometry
Cat # :
506308
Clone :
MP6-XT22
Item :
APC anti-mouse TNF-α
Isotype :
Rat IgG1, κ
Other Names :
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha
Size :
100 μg
Price (USD) :
264 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC
Immunogen :
E. coli -expressed, recombinant mouse TNF-α
Application Notes :
ELISA or ELISPOT Detection : The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 510804) as the capture antibody. ELISA Capture : The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF- (Cat. No. 575209) as the standard. Flow Cytometry 6,11,12 :The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. Neutralization 1,5,10,16,17 :The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse TNF- bioactivity in vivo and in vitro (Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include : Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 7-9 in vivo detection 5 , immunofluorescence, and immunocytochemistry. Note : For testing mouse TNF- in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430901) are specially developed and recommended.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA