PE anti-mouse TNF-α | BioLegend 506305 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE anti-mouse TNF-α

catalog :

506305

quantity :

25 μg

price :

60 USD

clonality :

monoclonal

host :

rat

conjugate :

clone name :

MP6-XT22

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	Meng Y, Zhou W, Jin L, Liu L, Chang K, Mei J, et al. RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization. Cell Death Dis. 2017;8:e3105 pubmed publisher
flow cytometry; mouse	OlguÃn J, FernÃ¡ndez J, Salinas N, JuÃ¡rez I, Rodriguez Sosa M, Campuzano J, et al. Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated Th1 immune response. Microbes Infect. 2015;17:586-95 pubmed publisher
	Lu Y, Lin Y, Huang X, Wu S, Wei J, Yang C. Oxaliplatin aggravates hepatic oxidative stress, inflammation and fibrosis in a non‑alcoholic fatty liver disease mouse model. Int J Mol Med. 2019;43:2398-2408 pubmed publisher
	Djokić V, Primus S, Akoolo L, Chakraborti M, Parveen N. Age-Related Differential Stimulation of Immune Response by Babesia microti and Borrelia burgdorferi During Acute Phase of Infection Affects Disease Severity. Front Immunol. 2018;9:2891 pubmed publisher
	Cong J, Wang X, Zheng X, Wang D, Fu B, Sun R, et al. Dysfunction of Natural Killer Cells by FBP1-Induced Inhibition of Glycolysis during Lung Cancer Progression. Cell Metab. 2018;28:243-255.e5 pubmed publisher
	Stutz M, Ojaimi S, Ebert G, Pellegrini M. Is Receptor-Interacting Protein Kinase 3 a Viable Therapeutic Target for Mycobacterium tuberculosis Infection?. Front Immunol. 2018;9:1178 pubmed publisher
	Chen D, Xie J, Fiskesund R, Dong W, Liang X, Lv J, et al. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype. Nat Commun. 2018;9:873 pubmed publisher
	Smith L, Boukhaled G, Condotta S, Mazouz S, Guthmiller J, Vijay R, et al. Interleukin-10 Directly Inhibits CD8+ T Cell Function by Enhancing N-Glycan Branching to Decrease Antigen Sensitivity. Immunity. 2018;48:299-312.e5 pubmed publisher
	de Mingo Pulido Á, Gardner A, Hiebler S, Soliman H, Rugo H, Krummel M, et al. TIM-3 Regulates CD103+ Dendritic Cell Function and Response to Chemotherapy in Breast Cancer. Cancer Cell. 2018;33:60-74.e6 pubmed publisher
	Ding Z, Habtetsion T, Cao Y, Li T, Liu C, Kuczma M, et al. Adjuvant IL-7 potentiates adoptive T cell therapy by amplifying and sustaining polyfunctional antitumor CD4+ T cells. Sci Rep. 2017;7:12168 pubmed publisher
	Baratin M, Simon L, Jorquera A, Ghigo C, Dembele D, Nowak J, et al. T Cell Zone Resident Macrophages Silently Dispose of Apoptotic Cells in the Lymph Node. Immunity. 2017;47:349-362.e5 pubmed publisher
	Knocke S, Fleischmann Mundt B, Saborowski M, Manns M, Kuhnel F, Wirth T, et al. Tailored Tumor Immunogenicity Reveals Regulation of CD4 and CD8 T Cell Responses against Cancer. Cell Rep. 2016;17:2234-2246 pubmed publisher
	Li B, Schmidt N. Epidermal Fatty Acid Binding Protein (E-FABP) Is Not Required for the Generation or Maintenance of Effector and Memory T Cells following Infection with Listeria monocytogenes. PLoS ONE. 2016;11:e0162427 pubmed publisher
	Liu J, Blake S, Harjunpää H, Fairfax K, Yong M, Allen S, et al. Assessing Immune-Related Adverse Events of Efficacious Combination Immunotherapies in Preclinical Models of Cancer. Cancer Res. 2016;76:5288-301 pubmed publisher
	Montes De Oca M, Kumar R, de Labastida Rivera F, Amante F, Sheel M, Faleiro R, et al. Blimp-1-Dependent IL-10 Production by Tr1 Cells Regulates TNF-Mediated Tissue Pathology. PLoS Pathog. 2016;12:e1005398 pubmed publisher
	Cabrera Mora M, Fonseca J, Singh B, Oliveira Ferreira J, Lima Junior J, Calvo Calle J, et al. Induction of Multifunctional Broadly Reactive T Cell Responses by a Plasmodium vivax Circumsporozoite Protein Recombinant Chimera. Infect Immun. 2015;83:3749-61 pubmed publisher
	Huang J, Wang L, Cong Z, Amoozgar Z, Kiner E, Xing D, et al. The PARP1 inhibitor BMN 673 exhibits immunoregulatory effects in a Brca1(-/-) murine model of ovarian cancer. Biochem Biophys Res Commun. 2015;463:551-6 pubmed publisher
	Charlton J, Tsoukatou D, Mamalaki C, Chatzidakis I. Programmed death 1 regulates memory phenotype CD4 T cell accumulation, inhibits expansion of the effector memory phenotype subset and modulates production of effector cytokines. PLoS ONE. 2015;10:e0119200 pubmed publisher
	Lu X, Ding Z, Cao Y, Liu C, Habtetsion T, Yu M, et al. Alkylating agent melphalan augments the efficacy of adoptive immunotherapy using tumor-specific CD4+ T cells. J Immunol. 2015;194:2011-21 pubmed publisher
	Asrat S, Dugan A, Isberg R. The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. PLoS Pathog. 2014;10:e1004229 pubmed publisher
	Ni P, Wang Y, Allen P. Both positive and negative effects on immune responses by expression of a second class II MHC molecule. Mol Immunol. 2014;62:199-208 pubmed publisher
	Dahlin J, Feinstein R, Cui Y, Heyman B, Hallgren J. CD11c+ cells are required for antigen-induced increase of mast cells in the lung. J Immunol. 2012;189:3869-77 pubmed publisher
	Akilov O, Kasuboski R, Carter C, McDowell M. The role of mannose receptor during experimental leishmaniasis. J Leukoc Biol. 2007;81:1188-96 pubmed

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

506305

Clone :

MP6-XT22

Item :

PE anti-mouse TNF-α

Isotype :

Rat IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha

Size :

25 μg

Price (USD) :

60 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

Immunogen :

E. coli -expressed, recombinant mouse TNF-α

Application Notes :

ELISA or ELISPOT Detection: The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 510804) as the capture antibody. ELISA Capture : The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. No. 575809) as the standard. Flow Cytometry 6,11,12 : The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-α-producing cells within mixed cell populations. Neutralization 1,5,10,16,17 : The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF-α. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse TNF-α bioactivity in vivo and in vitro (Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 7-9 , in vivo detection 5 , immunofluorescence, and immunocytochemistry. Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901 to 430906) are specially developed and recommended.

more info or order :

BioLegend product webpage