FITC anti-mouse TNF-α | BioLegend 506303 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

FITC anti-mouse TNF-α

catalog :

506303

quantity :

25 μg

price :

66 USD

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

MP6-XT22

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 5b	Susukida T, Kuwahara S, Song B, Kazaoka A, Aoki S, Ito K. Regulation of the immune tolerance system determines the susceptibility to HLA-mediated abacavir-induced skin toxicity. Commun Biol. 2021;4:1137 pubmed publisher
flow cytometry; mouse; loading ...; fig 5f	Xiao F, Ai G, Yan W, Wan X, Luo X, Ning Q. Intrahepatic recruitment of cytotoxic NK cells contributes to autoimmune hepatitis progression. Cell Immunol. 2018;327:13-20 pubmed publisher
flow cytometry; mouse; fig S2b	Takeda Y, Azuma M, Matsumoto M, Seya T. Tumoricidal efficacy coincides with CD11c up-regulation in antigen-specific CD8(+) T cells during vaccine immunotherapy. J Exp Clin Cancer Res. 2016;35:143 pubmed publisher
flow cytometry; mouse; fig 1	Lee Chang C, Bodogai M, Moritoh K, Chen X, Wersto R, Sen R, et al. Aging Converts Innate B1a Cells into Potent CD8+ T Cell Inducers. J Immunol. 2016;196:3385-97 pubmed publisher
	Marangoni F, Zhakyp A, Corsini M, Geels S, Carrizosa E, Thelen M, et al. Expansion of tumor-associated Treg cells upon disruption of a CTLA-4-dependent feedback loop. Cell. 2021;184:3998-4015.e19 pubmed publisher
	Trefzer A, Kadam P, Wang S, Pennavaria S, Lober B, Akçabozan B, et al. Dynamic adoption of anergy by antigen-exhausted CD4+ T cells. Cell Rep. 2021;34:108748 pubmed publisher
	Jiang L, Wang Y, Zhao J, Uehara M, Hou Q, Kasinath V, et al. Direct Tumor Killing and Immunotherapy through Anti-SerpinB9 Therapy. Cell. 2020;183:1219-1233.e18 pubmed publisher
	Kim S, Saeidi S, Zhong X, Gwak S, Muna I, Park S, et al. Breast cancer cell debris diminishes therapeutic efficacy through heme oxygenase-1-mediated inactivation of M1-like tumor-associated macrophages. Neoplasia. 2020;22:606-616 pubmed publisher
	Sun Y, Preiss N, Valenteros K, Kamal Y, Usherwood Y, Frost H, et al. Zbtb20 Restrains CD8 T Cell Immunometabolism and Restricts Memory Differentiation and Antitumor Immunity. J Immunol. 2020;205:2649-2666 pubmed publisher
	Acharya N, Madi A, Zhang H, Klapholz M, Escobar G, Dulberg S, et al. Endogenous Glucocorticoid Signaling Regulates CD8+ T Cell Differentiation and Development of Dysfunction in the Tumor Microenvironment. Immunity. 2020;53:658-671.e6 pubmed publisher
	Hamaidi I, Zhang L, Kim N, Wang M, Iclozan C, Fang B, et al. Sirt2 Inhibition Enhances Metabolic Fitness and Effector Functions of Tumor-Reactive T Cells. Cell Metab. 2020;32:420-436.e12 pubmed publisher
	Varikuti S, Singh B, Volpedo G, Ahirwar D, Jha B, Saljoughian N, et al. Ibrutinib treatment inhibits breast cancer progression and metastasis by inducing conversion of myeloid-derived suppressor cells to dendritic cells. Br J Cancer. 2020;122:1005-1013 pubmed publisher
	Steinmann S, Schoedsack M, Heinrich F, Breda P, Ochel A, Tiegs G, et al. Hepatic ILC2 activity is regulated by liver inflammation-induced cytokines and effector CD4+ T cells. Sci Rep. 2020;10:1071 pubmed publisher
	Jain A, Irizarry Caro R, McDaniel M, Chawla A, Carroll K, Overcast G, et al. T cells instruct myeloid cells to produce inflammasome-independent IL-1β and cause autoimmunity. Nat Immunol. 2020;21:65-74 pubmed publisher
	Zhang H, Christensen C, Dries R, Oser M, Deng J, Diskin B, et al. CDK7 Inhibition Potentiates Genome Instability Triggering Anti-tumor Immunity in Small Cell Lung Cancer. Cancer Cell. 2019;: pubmed publisher
	Hudson W, Gensheimer J, Hashimoto M, Wieland A, Valanparambil R, Li P, et al. Proliferating Transitory T Cells with an Effector-like Transcriptional Signature Emerge from PD-1+ Stem-like CD8+ T Cells during Chronic Infection. Immunity. 2019;51:1043-1058.e4 pubmed publisher
	Wan X, Zinselmeyer B, Zakharov P, Vomund A, Taniguchi R, Santambrogio L, et al. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Nature. 2018;560:107-111 pubmed publisher
	Bhattacharjee P, Keyel P. Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Sci Rep. 2018;8:6458 pubmed publisher
	Bowers E, Slaughter A, Frenette P, Kuick R, Pello O, Lucas D. Granulocyte-derived TNFα promotes vascular and hematopoietic regeneration in the bone marrow. Nat Med. 2018;24:95-102 pubmed publisher
	Dekhtiarenko I, Ratts R, Blatnik R, Lee L, Fischer S, Borkner L, et al. Peptide Processing Is Critical for T-Cell Memory Inflation and May Be Optimized to Improve Immune Protection by CMV-Based Vaccine Vectors. PLoS Pathog. 2016;12:e1006072 pubmed publisher
	Shang S, Siddiqui S, Bian Y, Zhao J, Wang C. Nonclassical MHC Ib-restricted CD8+ T Cells Recognize Mycobacterium tuberculosis-Derived Protein Antigens and Contribute to Protection Against Infection. PLoS Pathog. 2016;12:e1005688 pubmed publisher
	Collins S, Chan Li Y, Oh M, Vigeland C, Limjunyawong N, Mitzner W, et al. Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis. JCI Insight. 2016;1:e83116 pubmed
	Zhu M, Zou J, Li T, O Brien S, Zhang Y, Ogden S, et al. Differential Roles of Phospholipase D Proteins in FcÎµRI-Mediated Signaling and Mast Cell Function. J Immunol. 2015;195:4492-502 pubmed publisher
	Herrera R, Anderson C, Kumar K, Molina Cruz A, Nguyen V, Burkhardt M, et al. Reversible Conformational Change in the Plasmodium falciparum Circumsporozoite Protein Masks Its Adhesion Domains. Infect Immun. 2015;83:3771-80 pubmed publisher
	Chai D, Yue Y, Xu W, Dong C, Xiong S. AIM2 co-immunization favors specific multifunctional CD8(+) T cell induction and ameliorates coxsackievirus B3-induced chronic myocarditis. Antiviral Res. 2015;119:68-77 pubmed publisher
	Hira S, Mondal I, Manna P. Combined immunotherapy with whole tumor lysate-pulsed interleukin-15-activated dendritic cells and cucurbitacin I promotes strong CD8(+) T-cell responses and cures highly aggressive lymphoma. Cytotherapy. 2015;17:647-64 pubmed publisher
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product information

Antigen :

TNF-alpha

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

506303

Clone :

MP6-XT22

Item :

FITC anti-mouse TNF-α

Isotype :

Rat IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha

Size :

25 μg

Price (USD) :

66 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

FITC

Immunogen :

E. coli -expressed, recombinant mouse TNF-α

Application Notes :

ELISA or ELISPOT Detection : The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 510804) as the capture antibody. ELISA Capture : The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF- (Cat. No. 575209) as the standard. Flow Cytometry 6,11,12 :The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. Neutralization 1,5,10,16,17 :The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse TNF- bioactivity in vivo and in vitro (Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include : Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 7-9 in vivo detection 5 , immunofluorescence, and immunocytochemistry. Note : For testing mouse TNF- in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430901) are specially developed and recommended.

more info or order :

BioLegend product webpage