Ultra-LEAF™ Purified anti-mouse/rat/human MCP-1 | BioLegend 505912 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-mouse/rat/human MCP-1

catalog :

505912

quantity :

1 mg

price :

149 USD

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

2H5

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, neutralization, flow cytometry, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; mouse; ; fig 3g flow cytometry; mouse; loading ...; fig 4a	Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
	Feng H, Liu K, Shen X, Liang J, Wang C, Qiu W, et al. Targeting tumor cell-derived CCL2 as a strategy to overcome Bevacizumab resistance in ETV5+ colorectal cancer. Cell Death Dis. 2020;11:916 pubmed publisher
	Donado C, Cao A, Simmons D, Croker B, Brennan P, Brenner M. A Two-Cell Model for IL-1β Release Mediated by Death-Receptor Signaling. Cell Rep. 2020;31:107466 pubmed publisher

product information

Antigen :

CCL2

Apps. Abbrev. :

ELISA Capture, ELISPOT Capture, IHC, Neut, WB

Cat # :

505912

Clone :

2H5

Item :

Ultra-LEAF™ Purified anti-mouse/rat/human MCP-1

Isotype :

Hamster IgG

Other Names :

Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2

Size :

1 mg

Price (USD) :

149 USD

Reactivity :

Mouse, Rat, Human

Clonality :

Monoclonal

Host :

Hamster

Conjugate/Tag/Label :

ULEAF

Immunogen :

CHO-expressed, recombinant mouse MCP-1

Application Notes :

ELISA or ELISPOT Capture^1: The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.Flow Cytometry^2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization^1,4,6: The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.Additional reported applications (for the relevant formats) include: Western blotting^3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections^5, and acetone-fixed frozen tissue sections^7.;

more info or order :

BioLegend product webpage