Purified anti-mouse/rat/human MCP-1 | BioLegend 505902 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-mouse/rat/human MCP-1

catalog :

505902

quantity :

500 μg

price :

127 USD

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

2H5

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, dot blot, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; mouse; ; fig 3g flow cytometry; mouse; loading ...; fig 4a	Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
immunohistochemistry - paraffin section; mouse	Wan W, Liu Q, Lionakis M, Marino A, Anderson S, Swamydas M, et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res. 2015;106:478-87 pubmed publisher
dot blot; human; tbl s1	Lu Y, Xue Q, Eisele M, Sulistijo E, Brower K, Han L, et al. Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands. Proc Natl Acad Sci U S A. 2015;112:E607-15 pubmed publisher
	Jiao S, Subudhi S, Aparicio A, Ge Z, Guan B, Miura Y, et al. Differences in Tumor Microenvironment Dictate T Helper Lineage Polarization and Response to Immune Checkpoint Therapy. Cell. 2019;179:1177-1190.e13 pubmed publisher
	Bonnardel J, T Jonck W, Gaublomme D, Browaeys R, Scott C, Martens L, et al. Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche. Immunity. 2019;51:638-654.e9 pubmed publisher
	Lee B, Jo Y, Kim G, Ali L, Sohn D, Lee S, et al. Specific Inhibition of Soluble γc Receptor Attenuates Collagen-Induced Arthritis by Modulating the Inflammatory T Cell Responses. Front Immunol. 2019;10:209 pubmed publisher
	Arnold I, Artola Borán M, Tallón de Lara P, Kyburz A, Taube C, OTTEMANN K, et al. Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation. J Exp Med. 2018;215:2055-2072 pubmed publisher
	Hua J, Inomata T, Chen Y, Foulsham W, Stevenson W, Shiang T, et al. Pathological conversion of regulatory T cells is associated with loss of allotolerance. Sci Rep. 2018;8:7059 pubmed publisher
	Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, et al. Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget. 2018;9:11209-11226 pubmed publisher
	Van Krieken R, Marway M, Parthasarathy P, Mehta N, Ingram A, Gao B, et al. Inhibition of SREBP With Fatostatin Does Not Attenuate Early Diabetic Nephropathy in Male Mice. Endocrinology. 2018;159:1479-1495 pubmed publisher
	Rutar M, Natoli R, Valter K, Provis J. Early focal expression of the chemokine Ccl2 by Müller cells during exposure to damage-inducing bright continuous light. Invest Ophthalmol Vis Sci. 2011;52:2379-88 pubmed publisher
	Patsouris D, Neels J, Fan W, Li P, Nguyen M, Olefsky J. Glucocorticoids and thiazolidinediones interfere with adipocyte-mediated macrophage chemotaxis and recruitment. J Biol Chem. 2009;284:31223-35 pubmed publisher

product information

Antigen :

CCL2

Apps. Abbrev. :

ELISA Capture, IHC, Western Blotting

Cat # :

505902

Clone :

2H5

Item :

Purified anti-mouse/rat/human MCP-1

Isotype :

Hamster IgG

Other Names :

Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2

Size :

500 μg

Price (USD) :

127 USD

Reactivity :

Mouse, Rat, Human, Cross-Reactivity: Rhesus

Clonality :

Monoclonal

Host :

Hamster

Conjugate/Tag/Label :

PURE

Immunogen :

CHO-expressed, recombinant mouse MCP-1

Application Notes :

ELISA or ELISPOT Capture 1 : The Ultra-LEAF 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1. Flow Cytometry 2 : The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization 1,4,6 : The Ultra-LEAF purified antibody (Cat. Nos. 505911 505912) is recommended for neutralization. Additional reported applications (for the relevant formats) include: Western blotting 3 , immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections 5 , and acetone-fixed frozen tissue sections 7 .

more info or order :

BioLegend product webpage