Spark NIR™ 685 anti-mouse IFN-γ | BioLegend 505862 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Spark NIR™ 685 anti-mouse IFN-γ

catalog :

505862

quantity :

100 μg

price :

300 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

XMG1.2

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 6d, s4a	Hou X, Shi Y, Kang X, Rousu Z, Li D, Wang M, et al. Echinococcus granulosus: The establishment of the metacestode in the liver is associated with control of the CD4+ T-cell-mediated immune response in patients with cystic echinococcosis and a mouse model. Front Cell Infect Microbiol. 2022;12:983119 pubmed publisher
flow cytometry; mouse; 1:100	Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
flow cytometry; mouse; loading ...; fig 5a	Pan C, Wu Q, Wang S, Mei Z, Zhang L, Gao X, et al. Combination with Toll-like receptor 4 (TLR4) agonist reverses GITR agonism mediated M2 polarization of macrophage in Hepatocellular carcinoma. Oncoimmunology. 2022;11:2073010 pubmed publisher
flow cytometry; mouse; loading ...; fig 7b, s1a	Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
flow cytometry; mouse; fig s4d	Wang H, Wang Q, Yang C, Guo M, Cui X, Jing Z, et al. Bacteroides acidifaciens in the gut plays a protective role against CD95-mediated liver injury. Gut Microbes. 2022;14:2027853 pubmed publisher
flow cytometry; mouse; loading ...	Hamminger P, Marchetti L, Preglej T, Platzer R, Zhu C, Kamnev A, et al. Histone deacetylase 1 controls CD4+ T cell trafficking in autoinflammatory diseases. J Autoimmun. 2021;119:102610 pubmed publisher
flow cytometry; mouse; 1:100; fig 3e	Page N, Lemeille S, Vincenti I, Klimek B, Mariotte A, Wagner I, et al. Persistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling. Nat Commun. 2021;12:1009 pubmed publisher
flow cytometry; mouse; 1:200; loading ...	Yao C, Lou G, Sun H, Zhu Z, Sun Y, Chen Z, et al. BACH2 enforces the transcriptional and epigenetic programs of stem-like CD8+ T cells. Nat Immunol. 2021;22:370-380 pubmed publisher
flow cytometry; mouse; 1:50; fig s16a	Li Y, Sun Y, Kulke M, Hechler T, Van der Jeught K, Dong T, et al. Targeted immunotherapy for HER2-low breast cancer with 17p loss. Sci Transl Med. 2021;13: pubmed publisher
flow cytometry; mouse; loading ...; fig 4e, 4h	Meryk A, Grasse M, Balasco L, Kapferer W, Grubeck Loebenstein B, Pangrazzi L. Antioxidants N-Acetylcysteine and Vitamin C Improve T Cell Commitment to Memory and Long-Term Maintenance of Immunological Memory in Old Mice. Antioxidants (Basel). 2020;9: pubmed publisher

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505862

Clone :

XMG1.2

Item :

Spark NIR™ 685 anti-mouse IFN-γ

Isotype :

Rat IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

Size :

100 μg

Price (USD) :

300 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

SPKNIR_685

Immunogen :

E. coli -expressed, recombinant mouse IFN-γ

Application Notes :

ELISA 1-4,11,14 or ELISPOT 5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN- (Cat. No. 575309) as the standard. ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN- (Cat. No. 575309) as the standard. The LEAF purified antibody is suggested for ELISPOT capture (Cat. No. 505812). Flow Cytometry 7,8,12,13,16 : The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN- -producing cells within mixed cell populations. Neutralization 1-3,9,10 : The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IFN- bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections 6,22,23 , and immunocytochemistry. Note: For testing mouse IFN- in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

more info or order :

BioLegend product webpage