APC/Fire™ 750 anti-mouse IFN-γ | BioLegend 505860 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC/Fire™ 750 anti-mouse IFN-γ

catalog :

505860

quantity :

100 μg

price :

364 USD

clonality :

monoclonal

host :

rat

conjugate :

APC/Fire 750

clone name :

XMG1.2

reactivity :

mouse

application :

immunohistochemistry, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 29

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 6d, s4a	Hou X, Shi Y, Kang X, Rousu Z, Li D, Wang M, et al. Echinococcus granulosus: The establishment of the metacestode in the liver is associated with control of the CD4+ T-cell-mediated immune response in patients with cystic echinococcosis and a mouse model. Front Cell Infect Microbiol. 2022;12:983119 pubmed publisher
flow cytometry; mouse; 1:100	Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
flow cytometry; mouse; loading ...; fig 5a	Pan C, Wu Q, Wang S, Mei Z, Zhang L, Gao X, et al. Combination with Toll-like receptor 4 (TLR4) agonist reverses GITR agonism mediated M2 polarization of macrophage in Hepatocellular carcinoma. Oncoimmunology. 2022;11:2073010 pubmed publisher
flow cytometry; mouse; loading ...; fig 7b, s1a	Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
flow cytometry; mouse; fig s4d	Wang H, Wang Q, Yang C, Guo M, Cui X, Jing Z, et al. Bacteroides acidifaciens in the gut plays a protective role against CD95-mediated liver injury. Gut Microbes. 2022;14:2027853 pubmed publisher
flow cytometry; mouse; fig 2b	Tanaka Y, Onozato M, Mikami T, Kohwi Shigematsu T, Fukushima T, Kondo M. Increased Indoleamine 2,3-Dioxygenase Levels at the Onset of Sjögren's Syndrome in SATB1-Conditional Knockout Mice. Int J Mol Sci. 2021;22: pubmed publisher
flow cytometry; mouse; loading ...; fig 2h	Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
flow cytometry; mouse; loading ...; fig 4d	Zheng J, MEYERHOLZ D, Wong L, Gelb M, Murakami M, Perlman S. Coronavirus-specific antibody production in middle-aged mice requires phospholipase A2G2D. J Clin Invest. 2021;131: pubmed publisher
flow cytometry; mouse; fig 4b	Petty A, Dai R, Lapalombella R, Baiocchi R, Benson D, Li Z, et al. Hedgehog-induced PD-L1 on tumor-associated macrophages is critical for suppression of tumor-infiltrating CD8+ T cell function. JCI Insight. 2021;6: pubmed publisher
flow cytometry; mouse; loading ...	Hamminger P, Marchetti L, Preglej T, Platzer R, Zhu C, Kamnev A, et al. Histone deacetylase 1 controls CD4+ T cell trafficking in autoinflammatory diseases. J Autoimmun. 2021;119:102610 pubmed publisher
flow cytometry; mouse; 1:100; fig 3e	Page N, Lemeille S, Vincenti I, Klimek B, Mariotte A, Wagner I, et al. Persistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling. Nat Commun. 2021;12:1009 pubmed publisher
flow cytometry; mouse; 1:200; loading ...	Yao C, Lou G, Sun H, Zhu Z, Sun Y, Chen Z, et al. BACH2 enforces the transcriptional and epigenetic programs of stem-like CD8+ T cells. Nat Immunol. 2021;22:370-380 pubmed publisher
flow cytometry; mouse; 1:50; fig s16a	Li Y, Sun Y, Kulke M, Hechler T, Van der Jeught K, Dong T, et al. Targeted immunotherapy for HER2-low breast cancer with 17p loss. Sci Transl Med. 2021;13: pubmed publisher
flow cytometry; mouse; loading ...; fig 4e, 4h	Meryk A, Grasse M, Balasco L, Kapferer W, Grubeck Loebenstein B, Pangrazzi L. Antioxidants N-Acetylcysteine and Vitamin C Improve T Cell Commitment to Memory and Long-Term Maintenance of Immunological Memory in Old Mice. Antioxidants (Basel). 2020;9: pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 6a	Gao L, Li B, Wang J, Shen D, Yang M, Sun R, et al. Activation of Liver X Receptor α Sensitizes Mice to T-Cell Mediated Hepatitis. Hepatol Commun. 2020;4:1664-1679 pubmed publisher
flow cytometry; mouse; loading ...; fig 3c	Ning Y, Ding J, Sun X, Xie Y, Su M, Ma C, et al. HDAC9 deficiency promotes tumor progression by decreasing the CD8+ dendritic cell infiltration of the tumor microenvironment. J Immunother Cancer. 2020;8: pubmed publisher
blocking or activating experiments; mouse; ; fig 2b flow cytometry; mouse; loading ...; fig 2c	Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
flow cytometry; mouse; loading ...; fig 1f	Kumar A, Chamoto K, Chowdhury P, Honjo T. Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy. elife. 2020;9: pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 4d flow cytometry; mouse; loading ...; fig 4a, 4b	Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
flow cytometry; mouse; 1 ug/ml; loading ...; fig 2i	Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
flow cytometry; mouse; loading ...; fig 2c	Tizian C, Lahmann A, Hölsken O, Cosovanu C, Kofoed Branzk M, Heinrich F, et al. c-Maf restrains T-bet-driven programming of CCR6-negative group 3 innate lymphoid cells. elife. 2020;9: pubmed publisher
flow cytometry; mouse; loading ...; fig 6e	Williford J, Ishihara J, Ishihara A, Mansurov A, Hosseinchi P, Marchell T, et al. Recruitment of CD103+ dendritic cells via tumor-targeted chemokine delivery enhances efficacy of checkpoint inhibitor immunotherapy. Sci Adv. 2019;5:eaay1357 pubmed publisher
flow cytometry; mouse; loading ...; fig 4	Zhao Y, Yang Q, Jin C, Feng Y, Xie S, Xie H, et al. Changes of CD103-expressing pulmonary CD4+ and CD8+ T cells in S. japonicum infected C57BL/6 mice. BMC Infect Dis. 2019;19:999 pubmed publisher
flow cytometry; mouse; loading ...; fig 1d	Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
flow cytometry; mouse; fig 1a	Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
flow cytometry; mouse; loading ...; fig 1a	Poncette L, Chen X, Lorenz F, Blankenstein T. Effective NY-ESO-1-specific MHC II-restricted T cell receptors from antigen-negative hosts enhance tumor regression. J Clin Invest. 2019;129:324-335 pubmed publisher
	Hudson W, Gensheimer J, Hashimoto M, Wieland A, Valanparambil R, Li P, et al. Proliferating Transitory T Cells with an Effector-like Transcriptional Signature Emerge from PD-1+ Stem-like CD8+ T Cells during Chronic Infection. Immunity. 2019;51:1043-1058.e4 pubmed publisher
	Stathopoulou C, Gangaplara A, Mallett G, Flomerfelt F, Liniany L, Knight D, et al. PD-1 Inhibitory Receptor Downregulates Asparaginyl Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory T Cells. Immunity. 2018;49:247-263.e7 pubmed publisher
	Li C, DiSpirito J, Zemmour D, Spallanzani R, Kuswanto W, Benoist C, et al. TCR Transgenic Mice Reveal Stepwise, Multi-site Acquisition of the Distinctive Fat-Treg Phenotype. Cell. 2018;174:285-299.e12 pubmed publisher

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505860

Clone :

XMG1.2

Item :

APC/Fire™ 750 anti-mouse IFN-γ

Isotype :

Rat IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

Size :

100 μg

Price (USD) :

364 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

APC_F750

Immunogen :

E. coli -expressed, recombinant mouse IFN-γ

Application Notes :

ELISA 1-4,11,14 or ELISPOT 5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN- (Cat. No. 575309) as the standard. ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN- (Cat. No. 575309) as the standard. The LEAF purified antibody is suggested for ELISPOT capture (Cat. No. 505812). Flow Cytometry 7,8,12,13,16 : The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN- -producing cells within mixed cell populations. Neutralization 1-3,9,10 : The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IFN- bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections 6,22,23 , and immunocytochemistry. Note: For testing mouse IFN- in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

more info or order :

BioLegend product webpage