PE/Dazzle™ 594 anti-mouse IFN-γ | BioLegend 505846 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Dazzle™ 594 anti-mouse IFN-γ

catalog :

505846

quantity :

100 μg

price :

324 USD

clonality :

monoclonal

host :

rat

conjugate :

clone name :

XMG1.2

reactivity :

mouse

application :

immunohistochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 4e, 4h	Meryk A, Grasse M, Balasco L, Kapferer W, Grubeck Loebenstein B, Pangrazzi L. Antioxidants N-Acetylcysteine and Vitamin C Improve T Cell Commitment to Memory and Long-Term Maintenance of Immunological Memory in Old Mice. Antioxidants (Basel). 2020;9: pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 6a	Gao L, Li B, Wang J, Shen D, Yang M, Sun R, et al. Activation of Liver X Receptor α Sensitizes Mice to T-Cell Mediated Hepatitis. Hepatol Commun. 2020;4:1664-1679 pubmed publisher
flow cytometry; mouse; fig 1a	Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
flow cytometry; mouse; loading ...; fig 1d	Purvis H, Clarke F, Jordan C, Blanco C, Cornish G, Dai X, et al. Protein tyrosine phosphatase PTPN22 regulates IL-1β dependent Th17 responses by modulating dectin-1 signaling in mice. Eur J Immunol. 2018;48:306-315 pubmed publisher
flow cytometry; mouse; 1:333; loading ...; fig 7a	Papp S, Moderzynski K, Rauch J, Heine L, Kuehl S, Richardt U, et al. Liver Necrosis and Lethal Systemic Inflammation in a Murine Model of Rickettsia typhi Infection: Role of Neutrophils, Macrophages and NK Cells. PLoS Negl Trop Dis. 2016;10:e0004935 pubmed publisher
flow cytometry; mouse	Zhang Y, Mena P, Romanov G, Bliska J. Effector CD8+ T cells are generated in response to an immunodominant epitope in type III effector YopE during primary Yersinia pseudotuberculosis infection. Infect Immun. 2014;82:3033-44 pubmed publisher
flow cytometry; mouse; loading ...; fig 6b	Xia S, Wei J, Wang J, Sun H, Zheng W, Li Y, et al. A requirement of dendritic cell-derived interleukin-27 for the tumor infiltration of regulatory T cells. J Leukoc Biol. 2014;95:733-742 pubmed
flow cytometry; mouse	Nancy P, Tagliani E, Tay C, Asp P, Levy D, Erlebacher A. Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface. Science. 2012;336:1317-21 pubmed publisher
flow cytometry; mouse	Michaud M, Martins I, Sukkurwala A, Adjemian S, Ma Y, Pellegatti P, et al. Autophagy-dependent anticancer immune responses induced by chemotherapeutic agents in mice. Science. 2011;334:1573-7 pubmed publisher
	Zhang L, Wang W, Wang R, Zhang N, Shang H, Bi Y, et al. Reshaping the Immune Microenvironment by Oncolytic Herpes Simplex Virus in Murine Pancreatic Ductal Adenocarcinoma. Mol Ther. 2021;29:744-761 pubmed publisher
	Zhu Y, Smith D, Zhou Y, Li Y, Yu J, Lee D, et al. Development of Hematopoietic Stem Cell-Engineered Invariant Natural Killer T Cell Therapy for Cancer. Cell Stem Cell. 2019;25:542-557.e9 pubmed publisher
	Jardine L, Wiscombe S, Reynolds G, McDonald D, Fuller A, Green K, et al. Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace. Nat Commun. 2019;10:1999 pubmed publisher
	Orr M, Khandhar A, Seydoux E, Liang H, Gage E, Mikasa T, et al. Reprogramming the adjuvant properties of aluminum oxyhydroxide with nanoparticle technology. NPJ Vaccines. 2019;4:1 pubmed publisher
	Tsai S, Clemente Casares X, Zhou A, Lei H, Ahn J, Chan Y, et al. Insulin Receptor-Mediated Stimulation Boosts T Cell Immunity during Inflammation and Infection. Cell Metab. 2018;28:922-934.e4 pubmed publisher
	Kim M, van Dolleweerd C, Copland A, Paul M, Hofmann S, Webster G, et al. Molecular engineering and plant expression of an immunoglobulin heavy chain scaffold for delivery of a dengue vaccine candidate. Plant Biotechnol J. 2017;15:1590-1601 pubmed publisher
	Murphy M, Pattabiraman G, Manavalan T, Medvedev A. Deficiency in IRAK4 activity attenuates manifestations of murine Lupus. Eur J Immunol. 2017;47:880-891 pubmed publisher
	Moderzynski K, Papp S, Rauch J, Heine L, Kuehl S, Richardt U, et al. CD4+ T Cells Are as Protective as CD8+ T Cells against Rickettsia typhi Infection by Activating Macrophage Bactericidal Activity. PLoS Negl Trop Dis. 2016;10:e0005089 pubmed publisher
	MykkÃ¤nen O, Huotari A, Herzig K, Dunlop T, MykkÃ¤nen H, Kirjavainen P. Wild blueberries (Vaccinium myrtillus) alleviate inflammation and hypertension associated with developing obesity in mice fed with a high-fat diet. PLoS ONE. 2014;9:e114790 pubmed publisher
	Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
	Cui Y, Shao H, Sun D, Kaplan H. Regulation of interphotoreceptor retinoid-binding protein (IRBP)-specific Th1 and Th17 cells in anterior chamber-associated immune deviation (ACAID). Invest Ophthalmol Vis Sci. 2009;50:5811-7 pubmed publisher
	Tonkin D, He J, Barbour G, Haskins K. Regulatory T cells prevent transfer of type 1 diabetes in NOD mice only when their antigen is present in vivo. J Immunol. 2008;181:4516-22 pubmed
	Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed publisher
	Haring J, Jing X, Bollenbacher Reilley J, Xue H, Leonard W, Harty J. Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection. J Immunol. 2008;180:2855-62 pubmed
	Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed
	Lee J, Epardaud M, Sun J, Becker J, Cheng A, Yonekura A, et al. Peripheral antigen display by lymph node stroma promotes T cell tolerance to intestinal self. Nat Immunol. 2007;8:181-90 pubmed
	Ko S, Ko H, Chang W, Park S, Kweon M, Kang C. alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005;175:3309-17 pubmed
	Montfort M, Bouwer H, Wagner C, Hinrichs D. The development of functional CD8 T cell memory after Listeria monocytogenes infection is not dependent on CD40. J Immunol. 2004;173:4084-90 pubmed

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505846

Clone :

XMG1.2

Item :

PE/Dazzle™ 594 anti-mouse IFN-γ

Isotype :

Rat IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

Size :

100 μg

Price (USD) :

324 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PE_DZL594

Immunogen :

E. coli -expressed, recombinant mouse IFN-γ

Application Notes :

ELISA 1-4,11,14 or ELISPOT 5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN- (Cat. No. 575309) as the standard. ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN- (Cat. No. 575309) as the standard. The LEAF purified antibody is suggested for ELISPOT capture (Cat. No. 505812). Flow Cytometry 7,8,12,13,16 : The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN- -producing cells within mixed cell populations. Neutralization 1-3,9,10 : The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IFN- bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections 6,22,23 , and immunocytochemistry. Note: For testing mouse IFN- in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

more info or order :

BioLegend product webpage