Brilliant Violet 711™ anti-mouse IFN-γ | BioLegend 505835 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 711™ anti-mouse IFN-γ

catalog :

505835

quantity :

125 μL

price :

237 USD

clonality :

monoclonal

host :

rat

conjugate :

BV711

clone name :

XMG1.2

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 22

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 2a	Borkner L, Curham L, Wilk M, Moran B, Mills K. IL-17 mediates protective immunity against nasal infection with Bordetella pertussis by mobilizing neutrophils, especially Siglec-F+ neutrophils. Mucosal Immunol. 2021;14:1183-1202 pubmed publisher
flow cytometry; mouse	Nancy P, Tagliani E, Tay C, Asp P, Levy D, Erlebacher A. Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface. Science. 2012;336:1317-21 pubmed publisher
flow cytometry; mouse	Michaud M, Martins I, Sukkurwala A, Adjemian S, Ma Y, Pellegatti P, et al. Autophagy-dependent anticancer immune responses induced by chemotherapeutic agents in mice. Science. 2011;334:1573-7 pubmed publisher
	Bagati A, Kumar S, Jiang P, Pyrdol J, Zou A, Godicelj A, et al. Integrin αvβ6-TGFβ-SOX4 Pathway Drives Immune Evasion in Triple-Negative Breast Cancer. Cancer Cell. 2021;39:54-67.e9 pubmed publisher
	Li D, Bradley T, Cain D, Pedroza Pacheco I, Aggelakopoulou M, Parks R, et al. RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures. Immunohorizons. 2020;4:713-728 pubmed publisher
	Ye Y, Kuang X, Xie Z, Liang L, Zhang Z, Zhang Y, et al. Small-molecule MMP2/MMP9 inhibitor SB-3CT modulates tumor immune surveillance by regulating PD-L1. Genome Med. 2020;12:83 pubmed publisher
	McGinley A, Sutton C, Edwards S, Leane C, Decourcey J, Teijeiro A, et al. Interleukin-17A Serves a Priming Role in Autoimmunity by Recruiting IL-1β-Producing Myeloid Cells that Promote Pathogenic T Cells. Immunity. 2020;52:342-356.e6 pubmed publisher
	Angiari S, Runtsch M, Sutton C, Palsson McDermott E, Kelly B, Rana N, et al. Pharmacological Activation of Pyruvate Kinase M2 Inhibits CD4+ T Cell Pathogenicity and Suppresses Autoimmunity. Cell Metab. 2020;31:391-405.e8 pubmed publisher
	Trittel S, Chambers B, Heise U, Guzman C, Riese P. Key features and homing properties of NK cells in the liver are shaped by activated iNKT cells. Sci Rep. 2019;9:16362 pubmed publisher
	Chen E, Tay N, Brzostek J, Gascoigne N, Rybakin V. A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation. Front Immunol. 2019;10:1718 pubmed publisher
	Mancini M, Caignard G, Charbonneau B, Dumaine A, Wu N, Leiva Torres G, et al. Rel-Dependent Immune and Central Nervous System Mechanisms Control Viral Replication and Inflammation during Mouse Herpes Simplex Encephalitis. J Immunol. 2019;202:1479-1493 pubmed publisher
	Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
	MykkÃ¤nen O, Huotari A, Herzig K, Dunlop T, MykkÃ¤nen H, Kirjavainen P. Wild blueberries (Vaccinium myrtillus) alleviate inflammation and hypertension associated with developing obesity in mice fed with a high-fat diet. PLoS ONE. 2014;9:e114790 pubmed publisher
	Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
	Cui Y, Shao H, Sun D, Kaplan H. Regulation of interphotoreceptor retinoid-binding protein (IRBP)-specific Th1 and Th17 cells in anterior chamber-associated immune deviation (ACAID). Invest Ophthalmol Vis Sci. 2009;50:5811-7 pubmed publisher
	Tonkin D, He J, Barbour G, Haskins K. Regulatory T cells prevent transfer of type 1 diabetes in NOD mice only when their antigen is present in vivo. J Immunol. 2008;181:4516-22 pubmed
	Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed publisher
	Haring J, Jing X, Bollenbacher Reilley J, Xue H, Leonard W, Harty J. Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection. J Immunol. 2008;180:2855-62 pubmed
	Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed
	Lee J, Epardaud M, Sun J, Becker J, Cheng A, Yonekura A, et al. Peripheral antigen display by lymph node stroma promotes T cell tolerance to intestinal self. Nat Immunol. 2007;8:181-90 pubmed
	Ko S, Ko H, Chang W, Park S, Kweon M, Kang C. alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005;175:3309-17 pubmed
	Montfort M, Bouwer H, Wagner C, Hinrichs D. The development of functional CD8 T cell memory after Listeria monocytogenes infection is not dependent on CD40. J Immunol. 2004;173:4084-90 pubmed

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505835

Clone :

XMG1.2

Item :

Brilliant Violet 711™ anti-mouse IFN-γ

Isotype :

Rat IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

Size :

125 μL

Price (USD) :

237 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BV711

Immunogen :

E. coli -expressed, recombinant mouse IFN-γ

Application Notes :

ELISA 1-4,11,14 or ELISPOT 5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812). Flow Cytometry 7,8,12,13,16 : The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations. Neutralization 1-3,9,10 : The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-?. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections 6,22,23 , and immunocytochemistry. Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend s ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

more info or order :

BioLegend product webpage