Brilliant Violet 650™ anti-mouse IFN-γ | BioLegend 505832 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 650™ anti-mouse IFN-γ

catalog :

505832

quantity :

50 μg

price :

283 USD

clonality :

monoclonal

host :

rat

conjugate :

BV650

clone name :

XMG1.2

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 5d	Kotov D, Kotov J, Goldberg M, Jenkins M. Many Th Cell Subsets Have Fas Ligand-Dependent Cytotoxic Potential. J Immunol. 2018;200:2004-2012 pubmed publisher
	Zeng Q, Song J, Wang D, Sun X, Xiao Y, Zhang H, et al. Identification of Sorafenib as a Treatment for Type 1 Diabetes. Front Immunol. 2022;13:740805 pubmed publisher
	Dodagatta Marri E, Ma H, Liang B, Li J, Meyer D, Chen S, et al. Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy. Cell Rep. 2021;36:109309 pubmed publisher
	Loo Yau H, Bell E, Ettayebi I, de Almeida F, Boukhaled G, Shen S, et al. DNA hypomethylating agents increase activation and cytolytic activity of CD8+ T cells. Mol Cell. 2021;81:1469-1483.e8 pubmed publisher
	Lederer K, Castaño D, Gomez Atria D, Oguin T, Wang S, Manzoni T, et al. SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation. Immunity. 2020;53:1281-1295.e5 pubmed publisher
	Sekine T, Perez Potti A, Rivera Ballesteros O, Stralin K, Gorin J, Olsson A, et al. Robust T Cell Immunity in Convalescent Individuals with Asymptomatic or Mild COVID-19. Cell. 2020;183:158-168.e14 pubmed publisher
	Sendler M, van den Brandt C, Glaubitz J, Wilden A, Golchert J, Weiss F, et al. NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis. Gastroenterology. 2020;158:253-269.e14 pubmed publisher
	Willingham S, Ho P, Hotson A, Hill C, PICCIONE E, Hsieh J, et al. A2AR Antagonism with CPI-444 Induces Antitumor Responses and Augments Efficacy to Anti-PD-(L)1 and Anti-CTLA-4 in Preclinical Models. Cancer Immunol Res. 2018;6:1136-1149 pubmed publisher
	Cabrera Perez J, Babcock J, Dileepan T, Murphy K, Kucaba T, Badovinac V, et al. Gut Microbial Membership Modulates CD4 T Cell Reconstitution and Function after Sepsis. J Immunol. 2016;197:1692-8 pubmed publisher
	Cabrera Perez J, Condotta S, James B, Kashem S, Brincks E, Rai D, et al. Alterations in antigen-specific naive CD4 T cell precursors after sepsis impairs their responsiveness to pathogen challenge. J Immunol. 2015;194:1609-20 pubmed publisher
	MykkÃ¤nen O, Huotari A, Herzig K, Dunlop T, MykkÃ¤nen H, Kirjavainen P. Wild blueberries (Vaccinium myrtillus) alleviate inflammation and hypertension associated with developing obesity in mice fed with a high-fat diet. PLoS ONE. 2014;9:e114790 pubmed publisher
	Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
	Cui Y, Shao H, Sun D, Kaplan H. Regulation of interphotoreceptor retinoid-binding protein (IRBP)-specific Th1 and Th17 cells in anterior chamber-associated immune deviation (ACAID). Invest Ophthalmol Vis Sci. 2009;50:5811-7 pubmed publisher
	Tonkin D, He J, Barbour G, Haskins K. Regulatory T cells prevent transfer of type 1 diabetes in NOD mice only when their antigen is present in vivo. J Immunol. 2008;181:4516-22 pubmed
	Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed publisher
	Haring J, Jing X, Bollenbacher Reilley J, Xue H, Leonard W, Harty J. Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection. J Immunol. 2008;180:2855-62 pubmed
	Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed
	Lee J, Epardaud M, Sun J, Becker J, Cheng A, Yonekura A, et al. Peripheral antigen display by lymph node stroma promotes T cell tolerance to intestinal self. Nat Immunol. 2007;8:181-90 pubmed
	Ko S, Ko H, Chang W, Park S, Kweon M, Kang C. alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005;175:3309-17 pubmed
	Montfort M, Bouwer H, Wagner C, Hinrichs D. The development of functional CD8 T cell memory after Listeria monocytogenes infection is not dependent on CD40. J Immunol. 2004;173:4084-90 pubmed

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505832

Clone :

XMG1.2

Item :

Brilliant Violet 650™ anti-mouse IFN-γ

Isotype :

Rat IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

Size :

50 μg

Price (USD) :

283 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BV650

Immunogen :

E. coli -expressed, recombinant mouse IFN-γ

Application Notes :

ELISA 1-4,11,14 or ELISPOT 5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The LEAF purified antibody is suggested for ELISPOT capture (Cat. No. 505812). Flow Cytometry 7,8,12,13,16 : The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations. Neutralization 1-3,9,10 : The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-?. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections 6,22,23 , and immunocytochemistry. Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

more info or order :

BioLegend product webpage