LEAF™ Purified anti-mouse IFN-γ | BioLegend 505827 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse IFN-γ

catalog :

505827

quantity :

1 mg

price :

230 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

XMG1.2

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, neutralization, flow cytometry, blocking or activating experiments

citations: 51

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s4c	Metghalchi S, Vandestienne M, Haddad Y, Esposito B, Dairou J, Tedgui A, et al. Indoleamine 2 3-dioxygenase knockout limits angiotensin II-induced aneurysm in low density lipoprotein receptor-deficient mice fed with high fat diet. PLoS ONE. 2018;13:e0193737 pubmed publisher
flow cytometry; mouse; fig 6e	Lupfer C, Stokes K, Kuriakose T, Kanneganti T. Deficiency of the NOD-Like Receptor NLRC5 Results in Decreased CD8+ T Cell Function and Impaired Viral Clearance. J Virol. 2017;91: pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig 2a	Hasan Z, Koizumi S, Sasaki D, Yamada H, Arakaki N, Fujihara Y, et al. JunB is essential for IL-23-dependent pathogenicity of Th17 cells. Nat Commun. 2017;8:15628 pubmed publisher
flow cytometry; mouse; loading ...; fig 4f	Daley D, Mani V, Mohan N, Akkad N, Pandian G, Savadkar S, et al. NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma. J Exp Med. 2017;214:1711-1724 pubmed publisher
flow cytometry; mouse; loading ...; fig 1d	Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
flow cytometry; mouse; loading ...; fig 3f	Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
flow cytometry; mouse; loading ...; fig s2	Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, et al. BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J Exp Med. 2017;214:1313-1331 pubmed publisher
flow cytometry; mouse; loading ...; fig 4c	González Pérez G, Lamousé Smith E. Gastrointestinal Microbiome Dysbiosis in Infant Mice Alters Peripheral CD8+ T Cell Receptor Signaling. Front Immunol. 2017;8:265 pubmed publisher
flow cytometry; mouse; loading ...; fig 3f	Stacey M, Clare S, Clement M, Marsden M, Abdul Karim J, Kane L, et al. The antiviral restriction factor IFN-induced transmembrane protein 3 prevents cytokine-driven CMV pathogenesis. J Clin Invest. 2017;127:1463-1474 pubmed publisher
flow cytometry; mouse; loading ...; fig s7	Chamoto K, Chowdhury P, Kumar A, Sonomura K, Matsuda F, Fagarasan S, et al. Mitochondrial activation chemicals synergize with surface receptor PD-1 blockade for T cell-dependent antitumor activity. Proc Natl Acad Sci U S A. 2017;114:E761-E770 pubmed publisher
flow cytometry; mouse; fig s2a	Beyaz S, Kim J, Pinello L, Xifaras M, Hu Y, Huang J, et al. The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells. Nat Immunol. 2017;18:184-195 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Wei Y, Lu C, Chen J, Cui G, Wang L, Yu T, et al. High salt diet stimulates gut Th17 response and exacerbates TNBS-induced colitis in mice. Oncotarget. 2017;8:70-82 pubmed publisher
flow cytometry; mouse; loading ...; fig s2a	Coursey T, Bian F, Zaheer M, Pflugfelder S, Volpe E, de Paiva C. Age-related spontaneous lacrimal keratoconjunctivitis is accompanied by dysfunctional T regulatory cells. Mucosal Immunol. 2017;10:743-756 pubmed publisher
flow cytometry; mouse; loading ...; fig s5d	Swanson P, Hart G, Russo M, Nayak D, Yazew T, Pena M, et al. CD8+ T Cells Induce Fatal Brainstem Pathology during Cerebral Malaria via Luminal Antigen-Specific Engagement of Brain Vasculature. PLoS Pathog. 2016;12:e1006022 pubmed publisher
flow cytometry; mouse; loading ...; fig 6b	Yokota Nakatsuma A, Ohoka Y, Takeuchi H, Song S, Iwata M. Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties. Sci Rep. 2016;6:37914 pubmed publisher
flow cytometry; mouse; 1:333; loading ...; fig 7a	Papp S, Moderzynski K, Rauch J, Heine L, Kuehl S, Richardt U, et al. Liver Necrosis and Lethal Systemic Inflammation in a Murine Model of Rickettsia typhi Infection: Role of Neutrophils, Macrophages and NK Cells. PLoS Negl Trop Dis. 2016;10:e0004935 pubmed publisher
flow cytometry; mouse; loading ...; fig 3c	Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
blocking or activating experiments; mouse; fig 2 flow cytometry; mouse; fig 5	Nagashima H, Okuyama Y, Hayashi T, Ishii N, So T. TNFR-Associated Factors 2 and 5 Differentially Regulate the Instructive IL-6 Receptor Signaling Required for Th17 Development. J Immunol. 2016;196:4082-9 pubmed publisher
flow cytometry; mouse; loading ...; fig 1e	Holmkvist P, Pool L, Hägerbrand K, Agace W, Rivollier A. IL-18R?-deficient CD4(+) T cells induce intestinal inflammation in the CD45RB(hi) transfer model of colitis despite impaired innate responsiveness. Eur J Immunol. 2016;46:1371-82 pubmed publisher
flow cytometry; mouse; fig 2	Apostolidis S, RodrÃguez RodrÃguez N, SuÃ¡rez Fueyo A, Dioufa N, Ozcan E, CrispÃn J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
flow cytometry; mouse; fig 2	Kuipers H, Rieck M, Gurevich I, Nagy N, Butte M, Negrin R, et al. Hyaluronan synthesis is necessary for autoreactive T-cell trafficking, activation, and Th1 polarization. Proc Natl Acad Sci U S A. 2016;113:1339-44 pubmed publisher
flow cytometry; mouse; loading ...; fig 1a	Wu V, Smith A, You H, Nguyen T, Ferguson R, Taylor M, et al. Plasmacytoid dendritic cell-derived IFNÎ± modulates Th17 differentiation during early Bordetella pertussis infection in mice. Mucosal Immunol. 2016;9:777-86 pubmed publisher
flow cytometry; mouse; fig 4	Black L, Srivastava R, Schoeb T, Moore R, Barnes S, KABAROWSKI J. Cholesterol-Independent Suppression of Lymphocyte Activation, Autoimmunity, and Glomerulonephritis by Apolipoprotein A-I in Normocholesterolemic Lupus-Prone Mice. J Immunol. 2015;195:4685-98 pubmed publisher
flow cytometry; mouse; 1:200; fig 1	Xiao X, Shi X, Fan Y, Zhang X, Wu M, Lan P, et al. GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation. Nat Commun. 2015;6:8266 pubmed publisher
flow cytometry; mouse	Vuillefroy de Silly R, DucimetiÃ¨re L, Yacoub Maroun C, Dietrich P, Derouazi M, Walker P. Phenotypic switch of CD8(+) T cells reactivated under hypoxia toward IL-10 secreting, poorly proliferative effector cells. Eur J Immunol. 2015;45:2263-75 pubmed publisher
flow cytometry; mouse	Rao E, Zhang Y, Zhu G, Hao J, Persson X, Egilmez N, et al. Deficiency of AMPK in CD8+ T cells suppresses their anti-tumor function by inducing protein phosphatase-mediated cell death. Oncotarget. 2015;6:7944-58 pubmed
flow cytometry; mouse; fig 5	Cabrera Perez J, Condotta S, James B, Kashem S, Brincks E, Rai D, et al. Alterations in antigen-specific naive CD4 T cell precursors after sepsis impairs their responsiveness to pathogen challenge. J Immunol. 2015;194:1609-20 pubmed publisher
ELISA; mouse; fig 4	Li J, Liu D, Mou Z, Ihedioha O, Blanchard A, Jia P, et al. Deficiency of prolactin-inducible protein leads to impaired Th1 immune response and susceptibility to Leishmania major in mice. Eur J Immunol. 2015;45:1082-91 pubmed publisher
flow cytometry; mouse	Glatigny S, Duhen R, Arbelaez C, Kumari S, Bettelli E. Integrin alpha L controls the homing of regulatory T cells during CNS autoimmunity in the absence of integrin alpha 4. Sci Rep. 2015;5:7834 pubmed publisher
flow cytometry; mouse	Yuan X, Dee M, Altman N, Malek T. IL-2RÎ²-dependent signaling and CD103 functionally cooperate to maintain tolerance in the gut mucosa. J Immunol. 2015;194:1334-46 pubmed publisher
flow cytometry; mouse	Ikeda T, Hirata S, Takamatsu K, Haruta M, Tsukamoto H, Ito T, et al. Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells. PLoS ONE. 2014;9:e115198 pubmed publisher
flow cytometry; mouse	Stoycheva D, Deiser K, StÃ¤rck L, Nishanth G, SchlÃ¼ter D, Uckert W, et al. IFN-Î³ regulates CD8+ memory T cell differentiation and survival in response to weak, but not strong, TCR signals. J Immunol. 2015;194:553-9 pubmed publisher
flow cytometry; mouse	Peters A, Burkett P, Sobel R, Buckley C, Watson S, Bettelli E, et al. Podoplanin negatively regulates CD4+ effector T cell responses. J Clin Invest. 2015;125:129-40 pubmed publisher
flow cytometry; mouse	Wang X, Sumida H, Cyster J. GPR18 is required for a normal CD8Î±Î± intestinal intraepithelial lymphocyte compartment. J Exp Med. 2014;211:2351-9 pubmed publisher
flow cytometry; mouse; fig 5	Edwards C, Best S, Gun S, Claser C, James K, de Oca M, et al. Spatiotemporal requirements for IRF7 in mediating type I IFN-dependent susceptibility to blood-stage Plasmodium infection. Eur J Immunol. 2015;45:130-41 pubmed publisher
flow cytometry; mouse; 1:200	Chatterjee S, Thyagarajan K, Kesarwani P, Song J, Soloshchenko M, Fu J, et al. Reducing CD73 expression by IL1?-Programmed Th17 cells improves immunotherapeutic control of tumors. Cancer Res. 2014;74:6048-59 pubmed publisher
flow cytometry; mouse	Wei H, Nash W, Makrigiannis A, Brown M. Impaired NK-cell education diminishes resistance to murine CMV infection. Eur J Immunol. 2014;44:3273-82 pubmed publisher
flow cytometry; mouse; fig 6	Kobayashi T, Hamaguchi Y, Hasegawa M, Fujimoto M, Takehara K, Matsushita T. B cells promote tumor immunity against B16F10 melanoma. Am J Pathol. 2014;184:3120-9 pubmed publisher
flow cytometry; mouse	Hosking M, Flynn C, Whitton J. Antigen-specific naive CD8+ T cells produce a single pulse of IFN-? in vivo within hours of infection, but without antiviral effect. J Immunol. 2014;193:1873-85 pubmed publisher
flow cytometry; mouse	Madireddi S, Eun S, Lee S, Nemčovičová I, Mehta A, Zajonc D, et al. Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies. J Exp Med. 2014;211:1433-48 pubmed publisher
blocking or activating experiments; mouse; 10 ug/ml	Koga T, Hedrich C, Mizui M, Yoshida N, Otomo K, Lieberman L, et al. CaMK4-dependent activation of AKT/mTOR and CREM-? underlies autoimmunity-associated Th17 imbalance. J Clin Invest. 2014;124:2234-45 pubmed publisher
flow cytometry; mouse; fig 4	Yan J, Villarreal D, Racine T, Chu J, Walters J, Morrow M, et al. Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine. Vaccine. 2014;32:2833-42 pubmed publisher
flow cytometry; mouse	Misumi I, Whitmire J. B cell depletion curtails CD4+ T cell memory and reduces protection against disseminating virus infection. J Immunol. 2014;192:1597-608 pubmed publisher
flow cytometry; mouse; loading ...; fig 6b	Xia S, Wei J, Wang J, Sun H, Zheng W, Li Y, et al. A requirement of dendritic cell-derived interleukin-27 for the tumor infiltration of regulatory T cells. J Leukoc Biol. 2014;95:733-742 pubmed
flow cytometry; mouse; loading ...; fig 2	Brenndörfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol. 2014;192:1671-80 pubmed publisher
flow cytometry; mouse	McGuire D, Rowse A, Li H, Peng B, Sestero C, Cashman K, et al. CD5 enhances Th17-cell differentiation by regulating IFN-? response and ROR?t localization. Eur J Immunol. 2014;44:1137-42 pubmed publisher
blocking or activating experiments; mouse; 5 ug/ml	Lee P, Puppi M, Schluns K, Yu Lee L, Dong C, Lacorazza H. The transcription factor E74-like factor 4 suppresses differentiation of proliferating CD4+ T cells to the Th17 lineage. J Immunol. 2014;192:178-88 pubmed publisher
flow cytometry; mouse	Nancy P, Tagliani E, Tay C, Asp P, Levy D, Erlebacher A. Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface. Science. 2012;336:1317-21 pubmed publisher
flow cytometry; mouse	Michaud M, Martins I, Sukkurwala A, Adjemian S, Ma Y, Pellegatti P, et al. Autophagy-dependent anticancer immune responses induced by chemotherapeutic agents in mice. Science. 2011;334:1573-7 pubmed publisher
	MykkÃ¤nen O, Huotari A, Herzig K, Dunlop T, MykkÃ¤nen H, Kirjavainen P. Wild blueberries (Vaccinium myrtillus) alleviate inflammation and hypertension associated with developing obesity in mice fed with a high-fat diet. PLoS ONE. 2014;9:e114790 pubmed publisher
	Montfort M, Bouwer H, Wagner C, Hinrichs D. The development of functional CD8 T cell memory after Listeria monocytogenes infection is not dependent on CD40. J Immunol. 2004;173:4084-90 pubmed

product information

Antigen :

IFN-g

Apps. Abbrev. :

ELISA Capture, ELISPOT Capture, CyTOF®, Neut, Intracellular Staining for Flow Cytometry, IHC, Western Blotting

Cat # :

505827

Clone :

XMG1.2

Item :

LEAF™ Purified anti-mouse IFN-γ

Isotype :

Rat IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

Size :

1 mg

Price (USD) :

230 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

E. coli -expressed, recombinant mouse IFN-

Application Notes :

ELISA 1-4,11,14 or ELISPOT 5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN- (Cat. No. 575309) as the standard. ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN- (Cat. No. 575309) as the standard. The LEAF purified antibody is suggested for ELISPOT capture (Cat. No. 505812). Flow Cytometry 7,8,12,13,16 : The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN- -producing cells within mixed cell populations. Neutralization 1-3,9,10 : The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN- . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IFN- bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections 6,22,23 , and immunocytochemistry. Note: For testing mouse IFN- in serum, plasma or supernatant, BioLegend's ELISA Max Sets (Cat. No. 430801 to 430806) are specially developed and recommended.