Biotin anti-mouse IFN-γ | BioLegend 505803 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Biotin anti-mouse IFN-γ

catalog :

505803

quantity :

50 μg

price :

101 USD

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

XMG1.2

reactivity :

mouse

application :

ELISA, immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	Nancy P, Tagliani E, Tay C, Asp P, Levy D, Erlebacher A. Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface. Science. 2012;336:1317-21 pubmed publisher
flow cytometry; mouse	Michaud M, Martins I, Sukkurwala A, Adjemian S, Ma Y, Pellegatti P, et al. Autophagy-dependent anticancer immune responses induced by chemotherapeutic agents in mice. Science. 2011;334:1573-7 pubmed publisher
	Volpedo G, Pacheco Fernández T, Holcomb E, Zhang W, Lypaczewski P, Cox B, et al. Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasis. NPJ Vaccines. 2022;7:32 pubmed publisher
	Dong L, Chen C, Zhang Y, Guo P, Wang Z, Li J, et al. The loss of RNA N6-adenosine methyltransferase Mettl14 in tumor-associated macrophages promotes CD8+ T cell dysfunction and tumor growth. Cancer Cell. 2021;39:945-957.e10 pubmed publisher
	Berdasco C, Duhalde Vega M, Rosato Siri M, Goldstein J. Environmental Cues Modulate Microglial Cell Behavior Upon Shiga Toxin 2 From Enterohemorrhagic Escherichia coli Exposure. Front Cell Infect Microbiol. 2019;9:442 pubmed publisher
	Lew M, Noordin R, Monsur Alam Khan M, Tye G. Immune Stimulation of RAP domain binding protein (rTgRA15) from Toxoplasma gondii. Pathog Glob Health. 2018;112:387-394 pubmed publisher
	Wang P, Zhao P, Dong S, Xu T, He X, Chen M. An Albumin-binding Polypeptide Both Targets Cytotoxic T Lymphocyte Vaccines to Lymph Nodes and Boosts Vaccine Presentation by Dendritic Cells. Theranostics. 2018;8:223-236 pubmed publisher
	Peng Y. Forced expression of IL-7R promotes CD8 T cell cytotoxicity to self antigen. PLoS ONE. 2017;12:e0188112 pubmed publisher
	Mulder R, Banete A, Seaver K, Basta S. M(IL-4) Tissue Macrophages Support Efficient Interferon-Gamma Production in Antigen-Specific CD8+ T Cells with Reduced Proliferative Capacity. Front Immunol. 2017;8:1629 pubmed publisher
	Tanner S, Daft J, Hill S, Martin C, Lorenz R. Altered T-Cell Balance in Lymphoid Organs of a Mouse Model of Colorectal Cancer. J Histochem Cytochem. 2016;64:753-767 pubmed
	Cooley L, El Shikh M, Li W, Keim R, Zhang Z, Strauss J, et al. Impaired immunological synapse in sperm associated antigen 6 (SPAG6) deficient mice. Sci Rep. 2016;6:25840 pubmed publisher
	Thornton T, Delgado P, Chen L, Salas B, Krementsov D, Fernández M, et al. Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response. Nat Commun. 2016;7:10553 pubmed publisher
	MykkÃ¤nen O, Huotari A, Herzig K, Dunlop T, MykkÃ¤nen H, Kirjavainen P. Wild blueberries (Vaccinium myrtillus) alleviate inflammation and hypertension associated with developing obesity in mice fed with a high-fat diet. PLoS ONE. 2014;9:e114790 pubmed publisher
	Guo H, Baker S, Martinez Sobrido L, Topham D. Induction of CD8 T cell heterologous protection by a single dose of single-cycle infectious influenza virus. J Virol. 2014;88:12006-16 pubmed publisher
	Plantinga T, van Maren W, van Bergenhenegouwen J, Hameetman M, Nierkens S, Jacobs C, et al. Differential Toll-like receptor recognition and induction of cytokine profile by Bifidobacterium breve and Lactobacillus strains of probiotics. Clin Vaccine Immunol. 2011;18:621-8 pubmed publisher
	Peng X, Wu X, Chen L, Wang Z, Hu X, Song L, et al. Inhibition of phosphoinositide 3-kinase ameliorates dextran sodium sulfate-induced colitis in mice. J Pharmacol Exp Ther. 2010;332:46-56 pubmed publisher
	Montfort M, Bouwer H, Wagner C, Hinrichs D. The development of functional CD8 T cell memory after Listeria monocytogenes infection is not dependent on CD40. J Immunol. 2004;173:4084-90 pubmed

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

ELISA Detection, ELISPOT Detection, ICFC

Cat # :

505803

Clone :

XMG1.2

Item :

Biotin anti-mouse IFN-γ

Isotype :

Rat IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

Size :

50 μg

Price (USD) :

101 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BIOTIN

Immunogen :

E. coli -expressed, recombinant mouse IFN-γ

Application Notes :

ELISA^1-4,11,14 or ELISPOT^5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry^7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization^1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections^6,22,23, and immunocytochemistry.Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.;

more info or order :

BioLegend product webpage