Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Ultra-LEAF™ Purified anti-mouse IFN-γ

505710

5 mg

539 USD

monoclonal

rat

nonconjugated

R4-6A2

ELISA, neutralization, immunohistochemistry - frozen section

ELISA Capture, ELISPOT Capture, IHC-F, Neut

505710

R4-6A2

Ultra-LEAF™ Purified anti-mouse IFN-γ

Rat IgG1, κ

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

5 mg

539 USD

Mouse

Monoclonal

Rat

ULEAF

Partially-purified, native mouse IFN-γ

ELISA Capture^1-4,6,10,11 or ELISPOT Capture^5: The purified R4-6A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated XMG1.2 antibody (Cat. No. 505804) as the detecting antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT Capture, the suggested use of this antibody is 1.0 - 4.0 µg/mL.ELISA or ELISPOT Detection: The biotinylated R4-6A2 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified XMG1.2 antibody (Cat. Nos. 505802 & 505812) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.Neutralization^1,2,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. Nos. 505708 - 505713).Additional reported applications (for the relevant formats) include: immunohistochemical staining^3,6,8 of acetone-fixed frozen tissue sections.Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 and 430804) are specially developed and recommended.;