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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse IFN-γ
catalog :
505702
quantity :
500 μg
price :
136 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
R4-6A2
reactivity :
mouse
application :
ELISA, immunohistochemistry, flow cytometry, blocking or activating experiments
more info or order :
citations: 29
Published Application/Species/Sample/DilutionReference
  • ELISA; mouse; loading ...; fig 2e
Strandt H, Pinheiro D, Kaplan D, Wirth D, GRATZ I, Hammerl P, et al. Neoantigen Expression in Steady-State Langerhans Cells Induces CTL Tolerance. J Immunol. 2017;199:1626-1634 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Singh R, Miao T, Symonds A, Omodho B, Li S, Wang P. Egr2 and 3 Inhibit T-bet-Mediated IFN-? Production in T Cells. J Immunol. 2017;198:4394-4402 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig s4c
Angela M, Endo Y, Asou H, Yamamoto T, Tumes D, Tokuyama H, et al. Fatty acid metabolic reprogramming via mTOR-mediated inductions of PPAR? directs early activation of T cells. Nat Commun. 2016;7:13683 pubmed publisher
Kim H, Park J, Kim H, Kim C, Kang I, Lee H. Blood monocyte-derived CD169+ macrophages contribute to antitumor immunity against glioblastoma. Nat Commun. 2022;13:6211 pubmed publisher
Volpedo G, Pacheco Fernández T, Holcomb E, Zhang W, Lypaczewski P, Cox B, et al. Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasis. NPJ Vaccines. 2022;7:32 pubmed publisher
Chen Y, Liu R, Cui Y, Hettinghouse A, Fu W, Zhang L, et al. Penfluridol targets acid sphingomyelinase to inhibit TNF signaling and is therapeutic against inflammatory autoimmune diseases. Arthritis Res Ther. 2022;24:27 pubmed publisher
McCauley M, O Rourke J, Yáñez A, Markman J, Ho R, Wang X, et al. C9orf72 in myeloid cells suppresses STING-induced inflammation. Nature. 2020;585:96-101 pubmed publisher
Wu J, Weisshaar N, Hotz Wagenblatt A, Madi A, Ma S, Mieg A, et al. Skeletal muscle antagonizes antiviral CD8+ T cell exhaustion. Sci Adv. 2020;6:eaba3458 pubmed publisher
Lv Z, Zhang P, Li D, Qin M, Nie L, Wang X, et al. CD19-targeting fusion protein combined with PD1 antibody enhances anti-tumor immunity in mouse models. Oncoimmunology. 2020;9:1747688 pubmed publisher
Baumann D, Hägele T, Mochayedi J, Drebant J, Vent C, Blobner S, et al. Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy. Nat Commun. 2020;11:2176 pubmed publisher
Mohamed E, Sierra R, Trillo Tinoco J, Cao Y, Innamarato P, Payne K, et al. The Unfolded Protein Response Mediator PERK Governs Myeloid Cell-Driven Immunosuppression in Tumors through Inhibition of STING Signaling. Immunity. 2020;52:668-682.e7 pubmed publisher
Steinmann S, Schoedsack M, Heinrich F, Breda P, Ochel A, Tiegs G, et al. Hepatic ILC2 activity is regulated by liver inflammation-induced cytokines and effector CD4+ T cells. Sci Rep. 2020;10:1071 pubmed publisher
Zander R, SCHAUDER D, Xin G, Nguyen C, Wu X, ZAJAC A, et al. CD4+ T Cell Help Is Required for the Formation of a Cytolytic CD8+ T Cell Subset that Protects against Chronic Infection and Cancer. Immunity. 2019;51:1028-1042.e4 pubmed publisher
Harel M, Ortenberg R, Varanasi S, Mangalhara K, Mardamshina M, Markovits E, et al. Proteomics of Melanoma Response to Immunotherapy Reveals Mitochondrial Dependence. Cell. 2019;179:236-250.e18 pubmed publisher
Rauschenberger T, Schmitt V, Azeem M, Klein Hessling S, Murti K, Grän F, et al. T Cells Control Chemokine Secretion by Keratinocytes. Front Immunol. 2019;10:1917 pubmed publisher
Fulham M, Ratna A, Gerstein R, Kurt Jones E, Mandrekar P. Alcohol-induced adipose tissue macrophage phenotypic switching is independent of myeloid Toll-like receptor 4 expression. Am J Physiol Cell Physiol. 2019;317:C687-C700 pubmed publisher
Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
Mastroianni J, Stickel N, Andrlova H, Hanke K, Melchinger W, Duquesne S, et al. miR-146a Controls Immune Response in the Melanoma Microenvironment. Cancer Res. 2019;79:183-195 pubmed publisher
Deason K, Troutman T, Jain A, Challa D, Mandraju R, Brewer T, et al. BCAP links IL-1R to the PI3K-mTOR pathway and regulates pathogenic Th17 cell differentiation. J Exp Med. 2018;215:2413-2428 pubmed publisher
Godoy Calderón M, Salazar V, González Marcano E, Convit A. Autologous tumor cells/bacillus Calmette-Guérin/formalin-based novel breast cancer vaccine induces an immune antitumor response. Oncotarget. 2018;9:20222-20238 pubmed publisher
Meyer zu Horste G, Przybylski D, Schramm M, Wang C, Schnell A, Lee Y, et al. Fas Promotes T Helper 17 Cell Differentiation and Inhibits T Helper 1 Cell Development by Binding and Sequestering Transcription Factor STAT1. Immunity. 2018;48:556-569.e7 pubmed publisher
Vaartjes D, Nandakumar K, Holmdahl R, Raposo B. Increased salt exposure affects both lymphoid and myeloid effector functions, influencing innate-associated disease but not T-cell-associated autoimmunity. Immunology. 2018;: pubmed publisher
Liang D, Tian L, You R, Halpert M, Konduri V, Baig Y, et al. AIMp1 Potentiates TH1 Polarization and Is Critical for Effective Antitumor and Antiviral Immunity. Front Immunol. 2017;8:1801 pubmed publisher
Cardoso V, Chesne J, Ribeiro H, García Cassani B, Carvalho T, Bouchery T, et al. Neuronal regulation of type 2 innate lymphoid cells via neuromedin U. Nature. 2017;549:277-281 pubmed publisher
Barsheshet Y, Wildbaum G, Levy E, Vitenshtein A, Akinseye C, Griggs J, et al. CCR8+FOXp3+ Treg cells as master drivers of immune regulation. Proc Natl Acad Sci U S A. 2017;114:6086-6091 pubmed publisher
Woller N, Gürlevik E, Fleischmann Mundt B, Schumacher A, Knocke S, Kloos A, et al. Viral Infection of Tumors Overcomes Resistance to PD-1-immunotherapy by Broadening Neoantigenome-directed T-cell Responses. Mol Ther. 2015;23:1630-40 pubmed publisher
Lai L, Jin J, Goldschneider I. In vivo antitumor activity of a recombinant IL-7/HGFbeta hybrid cytokine in mice. Cancer Res. 2011;71:61-7 pubmed publisher
Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed
product information
Antigen :
IFN-gamma
Apps. Abbrev. :
ELISA Capture, IHC
Cat # :
505702
Clone :
R4-6A2
Item :
Purified anti-mouse IFN-γ
Isotype :
Rat IgG1, κ
Other Names :
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Size :
500 μg
Price (USD) :
136 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PURE
Immunogen :
Partially-purified, native mouse IFN-γ
Application Notes :
ELISA Capture^1-4,6,10,11 or ELISPOT Capture^5: The purified R4-6A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated XMG1.2 antibody (Cat. No. 505804) as the detecting antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT Capture, the suggested use of this antibody is 1.0 - 4.0 µg/mL.ELISA or ELISPOT Detection: The biotinylated R4-6A2 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified XMG1.2 antibody (Cat. Nos. 505802 & 505812) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.Neutralization^1,2,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. Nos. 505708 - 505713).Additional reported applications (for the relevant formats) include: immunohistochemical staining^3,6,8 of acetone-fixed frozen tissue sections.Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 and 430804) are specially developed and recommended.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA