Purified anti-mouse IFN-γ | BioLegend 505701 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-mouse IFN-γ

catalog :

505701

quantity :

50 μg

price :

83 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

R4-6A2

reactivity :

mouse

application :

ELISA, immunohistochemistry

more info or order :

BioLegend product webpage

citations: 7

Reference
Rauschenberger T, Schmitt V, Azeem M, Klein Hessling S, Murti K, Grän F, et al. T Cells Control Chemokine Secretion by Keratinocytes. Front Immunol. 2019;10:1917 pubmed publisher
Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
Godoy Calderón M, Salazar V, González Marcano E, Convit A. Autologous tumor cells/bacillus Calmette-Guérin/formalin-based novel breast cancer vaccine induces an immune antitumor response. Oncotarget. 2018;9:20222-20238 pubmed publisher
Singh R, Miao T, Symonds A, Omodho B, Li S, Wang P. Egr2 and 3 Inhibit T-bet-Mediated IFN-? Production in T Cells. J Immunol. 2017;198:4394-4402 pubmed publisher
Lai L, Jin J, Goldschneider I. In vivo antitumor activity of a recombinant IL-7/HGFbeta hybrid cytokine in mice. Cancer Res. 2011;71:61-7 pubmed publisher
Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

ELISA Capture, IHC

Cat # :

505701

Clone :

R4-6A2

Item :

Purified anti-mouse IFN-γ

Isotype :

Rat IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma

Size :

50 μg

Price (USD) :

83 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PURE

Immunogen :

Partially-purified, native mouse IFN-γ

Application Notes :

ELISA Capture 1-4,6,10,11 or ELISPOT Capture 5 : The purified R4-6A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated XMG1.2 antibody (Cat. No. 505804) as the detecting antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The Ultra-LEAF purified antibody is suggested for ELISPOT capture. For ELISPOT Capture, the suggested use of this antibody is 1.0 - 4.0 ug/mL. ELISA or ELISPOT Detection: The biotinylated R4-6A2 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified XMG1.2 antibody (Cat. Nos. 505802 505812) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. Neutralization 1,2,9 : The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. Nos. 505708 - 505713). Additional reported applications (for the relevant formats) include: immunohistochemical staining 3,6,8 of acetone-fixed frozen tissue sections. Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 430801 and 430804) are specially developed and recommended.

more info or order :

BioLegend product webpage