PE/Dazzle™ 594 anti-mouse GM-CSF | BioLegend 505421 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Dazzle™ 594 anti-mouse GM-CSF

catalog :

505421

quantity :

25 μg

price :

171 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MP1-22E9

reactivity :

mouse

application :

flow cytometry, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; mouse; fig 2a flow cytometry; mouse; loading ...; fig 1d	Vaillant L, Oster P, McMillan B, Orozco Fernandez E, Velin D. GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Helicobacter. 2022;27:e12875 pubmed publisher
flow cytometry; mouse; loading ...; fig s1c	Thiele Née Schrewe L, Guse K, Tietz S, Remlinger J, Demir S, Pedreiturria X, et al. Functional relevance of the multi-drug transporter abcg2 on teriflunomide therapy in an animal model of multiple sclerosis. J Neuroinflammation. 2020;17:9 pubmed publisher
	Hudson W, Gensheimer J, Hashimoto M, Wieland A, Valanparambil R, Li P, et al. Proliferating Transitory T Cells with an Effector-like Transcriptional Signature Emerge from PD-1+ Stem-like CD8+ T Cells during Chronic Infection. Immunity. 2019;51:1043-1058.e4 pubmed publisher
	Amir M, Chaudhari S, Wang R, Campbell S, Mosure S, Chopp L, et al. REV-ERBα Regulates TH17 Cell Development and Autoimmunity. Cell Rep. 2018;25:3733-3749.e8 pubmed publisher
	He Z, Zhang J, Huang Z, Du Q, Li N, Zhang Q, et al. Sumoylation of RORγt regulates TH17 differentiation and thymocyte development. Nat Commun. 2018;9:4870 pubmed publisher
	Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
	Zhang J, He Z, Sen S, Wang F, Zhang Q, Sun Z. TCF-1 Inhibits IL-17 Gene Expression To Restrain Th17 Immunity in a Stage-Specific Manner. J Immunol. 2018;200:3397-3406 pubmed publisher

product information

Antigen :

GM-CSF

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505421

Clone :

MP1-22E9

Item :

PE/Dazzle™ 594 anti-mouse GM-CSF

Isotype :

Rat IgG2a, κ

Other Names :

Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)

Size :

25 μg

Price (USD) :

171 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PE_DZL594

Immunogen :

Yeast-expressed, recombinant mouse GM-CSF

Application Notes :

ELISA or ELISPOT Capture 1,3-5 : The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry 8 : The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations. Neutralization 2-4 : The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408). Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 1,6,7 , and immunocytochemistry 8 .

more info or order :

BioLegend product webpage