APC anti-mouse GM-CSF | BioLegend 505413 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC anti-mouse GM-CSF

catalog :

505413

quantity :

25 μg

price :

95 USD

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

MP1-22E9

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 1d	Vaillant L, Oster P, McMillan B, Orozco Fernandez E, Velin D. GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Helicobacter. 2022;27:e12875 pubmed publisher
	Hinks T, Marchi E, Jabeen M, Olshansky M, Kurioka A, Pediongco T, et al. Activation and In Vivo Evolution of the MAIT Cell Transcriptome in Mice and Humans Reveals Tissue Repair Functionality. Cell Rep. 2019;28:3249-3262.e5 pubmed publisher
	Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher

product information

Antigen :

GM-CSF

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505413

Clone :

MP1-22E9

Item :

APC anti-mouse GM-CSF

Isotype :

Rat IgG2a, κ

Other Names :

Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)

Size :

25 μg

Price (USD) :

95 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

APC

Immunogen :

Yeast-expressed, recombinant mouse GM-CSF

Application Notes :

ELISA or ELISPOT Capture 1,3-5 : The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry 8 : The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations. Neutralization 2-4 : The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408). Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 1,6,7 , and immunocytochemistry 8 .

more info or order :

BioLegend product webpage