LEAF™ Purified anti-mouse GM-CSF | BioLegend 505408 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse GM-CSF

catalog :

505408

quantity :

500 μg

price :

250 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MP1-22E9

reactivity :

mouse

application :

immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s7	Vuckovic S, Minnie S, Smith D, Gartlan K, Watkins T, Markey K, et al. Bone marrow transplantation generates T cell-dependent control of myeloma in mice. J Clin Invest. 2019;129:106-121 pubmed publisher
flow cytometry; mouse; loading ...; fig 2e	Atretkhany K, Mufazalov I, Dunst J, Kuchmiy A, Gogoleva V, Andruszewski D, et al. Intrinsic TNFR2 signaling in T regulatory cells provides protection in CNS autoimmunity. Proc Natl Acad Sci U S A. 2018;115:13051-13056 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig s1d	He Z, Zhang J, Huang Z, Du Q, Li N, Zhang Q, et al. Sumoylation of RORγt regulates TH17 differentiation and thymocyte development. Nat Commun. 2018;9:4870 pubmed publisher
flow cytometry; mouse; loading ...; fig 1b	Prado C, Gaiazzi M, Gonzalez H, Ugalde V, Figueroa A, Osorio Barrios F, et al. Dopaminergic Stimulation of Myeloid Antigen-Presenting Cells Attenuates Signal Transducer and Activator of Transcription 3-Activation Favouring the Development of Experimental Autoimmune Encephalomyelitis. Front Immunol. 2018;9:571 pubmed publisher
blocking or activating experiments; mouse; fig 7q	Mitroulis I, Ruppova K, Wang B, Chen L, Grzybek M, Grinenko T, et al. Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity. Cell. 2018;172:147-161.e12 pubmed publisher
	Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
	Buchele V, Abendroth B, Büttner Herold M, Vogler T, Rothamer J, Ghimire S, et al. Targeting Inflammatory T Helper Cells via Retinoic Acid-Related Orphan Receptor Gamma t Is Ineffective to Prevent Allo-Response-Driven Colitis. Front Immunol. 2018;9:1138 pubmed publisher
	Zhang J, He Z, Sen S, Wang F, Zhang Q, Sun Z. TCF-1 Inhibits IL-17 Gene Expression To Restrain Th17 Immunity in a Stage-Specific Manner. J Immunol. 2018;200:3397-3406 pubmed publisher
	Li B, Wang X, Choi I, Wang Y, Liu S, Pham A, et al. miR-146a modulates autoreactive Th17 cell differentiation and regulates organ-specific autoimmunity. J Clin Invest. 2017;127:3702-3716 pubmed publisher
	Nussbaum K, Burkhard S, Ohs I, Mair F, Klose C, Arnold S, et al. Tissue microenvironment dictates the fate and tumor-suppressive function of type 3 ILCs. J Exp Med. 2017;214:2331-2347 pubmed publisher
	Hasan Z, Koizumi S, Sasaki D, Yamada H, Arakaki N, Fujihara Y, et al. JunB is essential for IL-23-dependent pathogenicity of Th17 cells. Nat Commun. 2017;8:15628 pubmed publisher
	Meierovics A, Cowley S. MAIT cells promote inflammatory monocyte differentiation into dendritic cells during pulmonary intracellular infection. J Exp Med. 2016;213:2793-2809 pubmed
	Kishi Y, Kondo T, Xiao S, Yosef N, Gaublomme J, Wu C, et al. Protein C receptor (PROCR) is a negative regulator of Th17 pathogenicity. J Exp Med. 2016;213:2489-2501 pubmed

product information

Antigen :

GM-CSF

Apps. Abbrev. :

ELISA Capture, ELISPOT Capture, Neut, ICC, IHC-F

Cat # :

505408

Clone :

MP1-22E9

Item :

LEAF™ Purified anti-mouse GM-CSF

Isotype :

Rat IgG2a, κ

Other Names :

Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)

Size :

500 μg

Price (USD) :

250 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

Yeast-expressed, recombinant mouse GM-CSF

Application Notes :

ELISA or ELISPOT Capture 1,3-5 : The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry 8 : The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations. Neutralization 2-4 : The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408). Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 1,6,7 , and immunocytochemistry 8 .