FITC anti-mouse GM-CSF | BioLegend 505404 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

FITC anti-mouse GM-CSF

catalog :

505404

quantity :

100 μg

price :

194 USD

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

MP1-22E9

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 8

Reference
Wagner A, Wang C, Fessler J, DeTomaso D, Avila Pacheco J, Kaminski J, et al. Metabolic modeling of single Th17 cells reveals regulators of autoimmunity. Cell. 2021;184:4168-4185.e21 pubmed publisher
Wu B, Zhang S, Guo Z, Bi Y, Zhou M, Li P, et al. The TGF-β superfamily cytokine Activin-A is induced during autoimmune neuroinflammation and drives pathogenic Th17 cell differentiation. Immunity. 2021;54:308-323.e6 pubmed publisher
Britton G, Contijoch E, Mogno I, Vennaro O, Llewellyn S, Ng R, et al. Microbiotas from Humans with Inflammatory Bowel Disease Alter the Balance of Gut Th17 and RORγt+ Regulatory T Cells and Exacerbate Colitis in Mice. Immunity. 2019;50:212-224.e4 pubmed publisher
Hernández Santos N, Wiesner D, Fites J, McDermott A, Warner T, Wuthrich M, et al. Lung Epithelial Cells Coordinate Innate Lymphocytes and Immunity against Pulmonary Fungal Infection. Cell Host Microbe. 2018;23:511-522.e5 pubmed publisher
Nakagawa S, Matsumoto M, Katayama Y, Oguma R, Wakabayashi S, Nygaard T, et al. Staphylococcus aureus Virulent PSMα Peptides Induce Keratinocyte Alarmin Release to Orchestrate IL-17-Dependent Skin Inflammation. Cell Host Microbe. 2017;22:667-677.e5 pubmed publisher
Lee P, Puppi M, Schluns K, Yu Lee L, Dong C, Lacorazza H. The transcription factor E74-like factor 4 suppresses differentiation of proliferating CD4+ T cells to the Th17 lineage. J Immunol. 2014;192:178-88 pubmed publisher
Larkin J, Renukaradhya G, Sriram V, Du W, Gervay Hague J, Brutkiewicz R. CD44 differentially activates mouse NK T cells and conventional T cells. J Immunol. 2006;177:268-79 pubmed
Gounni A, Hamid Q, Rahman S, Hoeck J, Yang J, Shan L. IL-9-mediated induction of eotaxin1/CCL11 in human airway smooth muscle cells. J Immunol. 2004;173:2771-9 pubmed

product information

Antigen :

GM-CSF

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505404

Clone :

MP1-22E9

Item :

FITC anti-mouse GM-CSF

Isotype :

Rat IgG2a, κ

Other Names :

Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)

Size :

100 μg

Price (USD) :

194 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

FITC

Immunogen :

Yeast-expressed, recombinant mouse GM-CSF

Application Notes :

ELISA or ELISPOT Capture 1,3-5 : The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry 8 : The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations. Neutralization 2-4 : The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408). Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 1,6,7 , and immunocytochemistry 8 .

more info or order :

BioLegend product webpage