Ultra-LEAF™ Purified anti-mouse IL-12/IL-23 p40 | BioLegend 505311 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-mouse IL-12/IL-23 p40

catalog :

505311

quantity :

50 mg

price :

2354 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

C17.8

reactivity :

mouse

application :

western blot, ELISA, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry

more info or order :

BioLegend product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:200; loading ...	Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
	Nomura A, Kobayashi T, Seo W, Ohno Oishi M, Kakugawa K, Muroi S, et al. Identification of a novel enhancer essential for Satb1 expression in TH2 cells and activated ILC2s. Life Sci Alliance. 2023;6: pubmed publisher
	Luo W, Yu S, Chang C, Chien M, Chang Y, Liao K, et al. HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages. elife. 2022;11: pubmed publisher
	Minns D, Smith K, Alessandrini V, Hardisty G, Melrose L, Jackson Jones L, et al. The neutrophil antimicrobial peptide cathelicidin promotes Th17 differentiation. Nat Commun. 2021;12:1285 pubmed publisher
	Imanishi T, Unno M, Kobayashi W, Yoneda N, Akira S, Saito T. mTORC1 Signaling Controls TLR2-Mediated T-Cell Activation by Inducing TIRAP Expression. Cell Rep. 2020;32:107911 pubmed publisher
	Price C, Jones S, Mihelcic M, Santic M, Abu Kwaik Y. Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector. Cell Host Microbe. 2020;27:571-584.e7 pubmed publisher

product information

Antigen :

IL-12/IL-23 p40

Apps. Abbrev. :

ELISA, Neut, ICFC, IP, WB

Cat # :

505311

Clone :

C17.8

Item :

Ultra-LEAF™ Purified anti-mouse IL-12/IL-23 p40

Isotype :

Rat IgG2a, κ

Other Names :

Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)

Size :

50 mg

Price (USD) :

2354 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

ULEAF

Immunogen :

CHO expressed, recombinant mouse IL-12 p70

Application Notes :

ELISA Detection^1,2,6-9 or ELISPOT Detection: The biotinylated C17.8 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified C15.6 antibody (Cat. No. 505202/505208) as capture antibody and recombinant mouse IL-12/IL-23 p40 (Cat. No. 576909) or recombinant mouse IL-12 p70 (Cat. No. 577009) as the standard.Neutralization^1,2,4,5,6,10: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-12 bioactivity in vivo and in vitro (Cat. No. 505304). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505308) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for relevant formats of this clone) include: Western blotting^3, immunoprecipitation^3, and intracellular immunofluorescent staining and flow cytometric analysis.Note: For testing mouse IL-12 p40 (monomer, dimer, heterodimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.;

more info or order :

BioLegend product webpage