LEAF™ Purified anti-mouse IL-12/IL-23 p40 | BioLegend 505304 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse IL-12/IL-23 p40

catalog :

505304

quantity :

500 μg

price :

290 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

C17.8

reactivity :

mouse

application :

western blot, ELISA, neutralization, immunoprecipitation, blocking or activating experiments

citations: 1

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; mouse; fig 5	Nagashima H, Okuyama Y, Hayashi T, Ishii N, So T. TNFR-Associated Factors 2 and 5 Differentially Regulate the Instructive IL-6 Receptor Signaling Required for Th17 Development. J Immunol. 2016;196:4082-9 pubmed publisher

product information

Antigen :

IL-12/IL-23

Apps. Abbrev. :

ELISA, Neut, Intracellular Staining for Flow Cytometry, IP, Western Blotting

Cat # :

505304

Clone :

C17.8

Item :

LEAF™ Purified anti-mouse IL-12/IL-23 p40

Isotype :

Rat IgG2a, κ

Other Names :

Interleukin-12 p40, Interleukin-23 p40, CLMF p40, NKSF p40, TcMF p40, TSF p40

Size :

500 μg

Price (USD) :

290 USD

Reactivity :

Mouse, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

CHO expressed, recombinant mouse IL-12 p70

Application Notes :

ELISA Detection 1,2,6-9 or ELISPOT Detection: The biotinylated C17.8 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified C15.6 antibody (Cat. No. 505202/505208) as capture antibody and recombinant mouse IL-12/IL-23 p40 (Cat. No. 576909) or recombinant mouse IL-12 p70 (Cat. No. 577009) as the standard. Neutralization 1,2,4,5,6,10 : The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IL-12 bioactivity in vivo and in vitro (Cat. No. 505304). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 505308) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug). Additional reported applications (for relevant formats of this clone) include: Western blotting 3 , immunoprecipitation 3 , and intracellular immunofluorescent staining and flow cytometric analysis. Note: For testing mouse IL-12 p40 (monomer, dimer, heterodimer) in serum or plasma, BioLegend's ELISA Max Sets (Cat. No. 431601 to 431606) are specially developed and recommended.